Estimation, Decision and Applications to Target Tracking

This dissertation mainly consists of three parts. The first part proposes generalized linear minimum mean-square error (GLMMSE) estimation for nonlinear point estimation. The second part proposes a recursive joint decision and estimation (RJDE) algorithm for joint decision and estimation (JDE). The third part analyzes the performance of sequential probability ratio test (SPRT) when the log-likelihood ratios (LLR) are independent but not identically distributed. The linear minimum mean-square error (LMMSE) estimation plays an important role in nonlinear estimation. It searches for the best estimator in the set of all estimators that are linear in the measurement. A GLMMSE estimation framework is proposed in this disser- tation. It employs a vector-valued measurement transform function (MTF) and finds the best estimator among all estimators that are linear in MTF. Several design guidelines for the MTF based on a numerical example were provided. A RJDE algorithm based on a generalized Bayes risk is proposed in this dissertation for dynamic JDE problems. It is computationally efficient for dynamic problems where data are made available sequentially. Further, since existing performance measures for estimation or decision are effective to evaluate JDE algorithms, a joint performance measure is proposed for JDE algorithms for dynamic problems. The RJDE algorithm is demonstrated by applications to joint tracking and classification as well as joint tracking and detection in target tracking. The characteristics and performance of SPRT are characterized by two important functions—operating characteristic (OC) and average sample number (ASN). These two functions have been studied extensively under the assumption of independent and identically distributed (i.i.d.) LLR, which is too stringent for many applications. This dissertation relaxes the requirement of identical distribution. Two inductive equations governing the OC and ASN are developed. Unfortunately, they have non-unique solutions in the general case. They do have unique solutions in two special cases: (a) the LLR sequence converges in distributions and (b) the LLR sequence has periodic distributions. Further, the analysis can be readily extended to evaluate the performance of the truncated SPRT and the cumulative sum test

Application of advanced fluorescence microscopy and spectroscopy in live-cell imaging

Since its inception, fluorescence microscopy has been a key source of discoveries in cell biology. Advancements in fluorophores, labeling techniques and instrumentation have made fluorescence microscopy a versatile quantitative tool for studying dynamic processes and interactions both in vitro and in live-cells. In this thesis, I apply quantitative fluorescence microscopy techniques in live-cell environments to investigate several biological processes. To study Gag processing in HIV-1 particles, fluorescence lifetime imaging microscopy and single particle tracking are combined to follow nascent HIV-1 virus particles during assembly and release on the plasma membrane of living cells. Proteolytic release of eCFP embedded in the Gag lattice of immature HIV-1 virus particles results in a characteristic increase in its fluorescence lifetime. Gag processing and rearrangement can be detected in individual virus particles using this approach. In another project, a robust method for quantifying Förster resonance energy transfer in live-cells is developed to allow direct comparison of live-cell FRET experiments between laboratories. Finally, I apply image fluctuation spectroscopy to study protein behavior in a variety of cellular environments. Image cross-correlation spectroscopy is used to study the oligomerization of CXCR4, a G-protein coupled receptor on the plasma membrane. With raster image correlation spectroscopy, I measure the diffusion of histones in the nucleoplasm and heterochromatin domains of the nuclei of early mouse embryos. The lower diffusion coefficient of histones in the heterochromatin domain supports the conclusion that heterochromatin forms a liquid phase-separated domain. The wide range of topics covered in this thesis demonstrate that fluorescence microscopy is more than just an imaging tool but also a powerful instrument for the quantification and elucidation of dynamic cellular processes

Electronic warfare self-protection of battlefield helicopters : a holistic view

The dissertation seeks to increase understanding of electronic warfare (EW) self-protection (EWSP) of battlefield helicopters by taking a holistic (systems) view on EWSP. It also evaluates the methodologies used in the research and their suitability as descriptive tools in communication between various EWSP stakeholders. The interpretation of the term "holistic view" is a central theme to the dissertation. The research methodology is bottom-up – which is necessary since no previous work exists that could guide the study – and progresses from analysis to synthesis. Initially several methods are evaluated for presenting findings on EWSP, including high-level system simulation such as Forrester system dynamics (FSD). The analysis is conducted by a comprehensive literature review on EW and other areas that are believed to be of importance to the holistic view. Combat scenarios, intelligence, EW support, validation, training, and delays have major influence on the effectiveness of the EWSP suite; while the initial procurement decision on the EWSP suite sets limits to what can be achieved later. The need for a vast support structure for EWSP means that countries with limited intelligence and other resources become dependent on allies for support; that is, the question of EWSP effectiveness becomes political. The synthesis shows that a holistic view on EWSP of battlefield helicopters cannot be bounded in the temporal or hierarchical (organizational) senses. FSD is found to be helpful as a quality assurance tool, but refinements are needed if FSD is to be useful as a general discussion tool. The area of survivability is found to be the best match for the holistic view – for an EWSP suprasystem. A global survivability paradigm is defined as the ultimate holistic view on EWSP. It is suggested that future research should be top-down and aiming at promoting the global survivability paradigm. The survivability paradigm would give EWSP a natural framework in which its merits can be assessed objectively.reviewe

Energy: A continuing bibliography with indexes, issue 15

This bibliography lists 1112 reports, articles, and other documents introduced into the NASA scientific and technical information system from July 1, 1977 through September 30, 1977

Energy: A continuing bibliography with indexes, issue 13

This bibliography lists 1036 reports, articles, and other documents introduced into the NASA scientific and technical information system from January 1, 1977 through March 31, 1977

Energy: A continuing bibliography with indexes, issue 19

A bibliographical list of 1339 reports, articles, and other documents introduced into the NASA scientific and technical information system from July 1, 1978 through September 30, 1978 are presented