Early detection of tomato bacterial canker by reflectance indices

Early detection of infected asymptomatic plants could prevent the dissemination of tomato bacterial canker throughout the crop and, thus, reduce associated economic losses. Reflectance spectroscopy has been proposed for the detection of diseases in plants, mainly caused by foliar fungal pathogens. In contrast, bacterial canker is a vascular disease that does not produce visible foliar symptoms until it is too late to avoid contagion to other plants. The effects of the disease on the conductivity of the xylem have an impact on the water content of the leaves; initially, a subtle effect that cannot be assessed with the naked eye but can be evaluated by reflectance spectroscopy. The objective of this work was to identify indices, based on the reflectance spectral signature of the plants, for the detection of tomato plants affected by bacterial canker prior to the appearance of symptoms. To verify that the proposed methodology is applicable to a broad range of tomato genotypes, two experiments were carried out with different tomato cultivars grown in pots and another on the ground in a greenhouse. Plants were inoculated with the pathogenic bacteria and the reflectance spectrum of the apical leaflet of two leaves per plant were obtained before symptoms expression; several spectral indices were calculated from the reflectance spectra. Three indices, of the shortwave-infrared zone, allowed the detection of bacterial canker inoculated plants in a fast and non-destructive way, up to one week before symptoms were visible: Normalized Difference Water Index (NDWI), Simple Ratio of Water Index (SRWI) and Water Index 1 180 (WI1180). Our research demonstrates, for the first time, the usefulness of spectral indices sensitive to water content for the identification of diseased plants. These indices arise as a promising tool for the early detection of vascular plant diseases, that could be integrated into sustainable management plans.Fil: Cordon, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Andrade, Carolina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Barbara, Lucía. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; ArgentinaFil: Romero, Ana María. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentin

Past and future of plant stress detection: an overview from remote sensing to Positron Emission Tomography

Plant stress detection is considered one of the most critical areas for the improvement of crop yield in the compelling worldwide scenario, dictated by both the climate change and the geopolitical consequences of the Covid-19 epidemics. A complicated interconnection of biotic and abiotic stressors affect plant growth, including water, salt, temperature, light exposure, nutrients availability, agrochemicals, air and soil pollutants, pests and diseases. In facing this extended panorama, the technology choice is manifold. On the one hand, quantitative methods, such as metabolomics, provide very sensitive indicators of most of the stressors, with the drawback of a disruptive approach, which prevents follow up and dynamical studies. On the other hand qualitative methods, such as fluorescence, thermography and VIS/NIR reflectance, provide a non-disruptive view of the action of the stressors in plants, even across large fields, with the drawback of a poor accuracy. When looking at the spatial scale, the effect of stress may imply modifications from DNA level (nanometers) up to cell (micrometers), full plant (millimeters to meters) and entire field (kilometers). While quantitative techniques are sensitive to the smallest scales, only qualitative approaches can be used for the larger ones. Emerging technologies from nuclear and medical physics, such as computed tomography, magnetic resonance imaging and positron emission tomography, are expected to bridge the gap of quantitative non disruptive morphologic and functional measurements at larger scale. In this review we analyze the landscape of the different technologies nowadays available, showing the benefits of each approach in plant stress detection, with a particular focus on the gaps, which will be filled in the nearby future by the emerging nuclear physics approaches to agriculture

Novel Vegetation Indices to Identify Broccoli Plants Infected With Xanthomonas campestris pv. campestris

A rapid diagnosis of black rot in brassicas, a devastating disease caused by Xanthomonas campestris pv. campestris (Xcc), would be desirable to avoid significant crop yield losses. The main aim of this work was to develop a method of detection of Xcc infection on broccoli leaves. Such method is based on the use of imaging sensors that capture information about the optical properties of leaves and provide data that can be implemented on machine learning algorithms capable of learning patterns. Based on this knowledge, the algorithms are able to classify plants into categories (healthy and infected). To ensure the robustness of the detection method upon future alterations in climate conditions, the response of broccoli plants to Xcc infection was analyzed under a range of growing environments, taking current climate conditions as reference. Two projections for years 2081–2100 were selected, according to the Assessment Report of Intergovernmental Panel on Climate Change. Thus, the response of broccoli plants to Xcc infection and climate conditions has been monitored using leaf temperature and five conventional vegetation indices (VIs) derived from hyperspectral reflectance. In addition, three novel VIs, named diseased broccoli indices (DBI1-DBI3), were defined based on the spectral reflectance signature of broccoli leaves upon Xcc infection. Finally, the nine parameters were implemented on several classifying algorithms. The detection method offering the best performance of classification was a multilayer perceptron-based artificial neural network. This model identified infected plants with accuracies of 88.1, 76.9, and 83.3%, depending on the growing conditions. In this model, the three Vis described in this work proved to be very informative parameters for the disease detection. To our best knowledge, this is the first time that future climate conditions have been taken into account to develop a robust detection model using classifying algorithms.MCIN/AEI RTI2018-094652-B-I00ERDF: A way of making EuropeConsejo Superior de Investigaciones Cientificas (CSIC) through the Unidad de Recursos de Informacion Cientifica para la Investigacion (URICI

Remote Sensing as a Precision Farming Tool in the Nile Valley, Egypt

Detecting stress in plants resulting from different stressors including nitrogen deficiency, salinity, moisture, contamination and diseases, is crucial in crop production. In the Nile Valley, crop production is hindered perhaps more fundamentally by issues of water supply and salinity. Predicting stress in crops by conventional methods is tedious, laborious and costly and is perhaps unreliable in providing a spatial context of stress patterns. Accurate and quick monitoring techniques for crop status to detect stress in crops at early growth stages are needed to maximize crop productivity. In this context, remotely sensed data may provide a useful tool in precision farming. This research aims to evaluate the role of in situ hyperspectral and high spatial resolution satellite remote sensing data to detect stress in wheat and maize crops and assess whether moisture induced stress can be distinguished from salinity induced stress spectrally. A series of five greenhouse based experiments on wheat and maize were undertaken subjecting both crops to a range of salinity and moisture stress levels. Spectroradiometry measurements were collected at different growth stages of each crop to assess the relationship between crop biophysical and biochemical properties and reflectance measurements from plant canopies. Additionally, high spatial resolution satellite images including two QuickBird, one ASTER and two SPOT HRV were acquired in south-west Alexandria, Egypt to assess the potential of high spectral and spatial resolution satellite imagery to detect stress in wheat and maize at local and regional scales. Two field work visits were conducted in Egypt to collect ground reference data and coupled with Hyperion imagery acquisition, during winter and summer seasons of 2007 in March (8-30: wheat) and July (12-17: maize). Despite efforts, Hyperion imagery was not acquired due to factors out with the control of this research. Strong significant correlations between crop properties and different vegetation indices derived from both ground based and satellite platforms were observed. RDVI showed a sensitive index to different wheat properties (r > 0.90 with different biophysical properties). In maize, GNDVIbr and Cgreen had strong significant correlations with maize biophysical properties (r > 0.80). PCA showed the possibility to distinguish between moisture and salinity induced stress at the grain filling stages. The results further showed that a combined approach of high (2-5 m) and moderate (15-20) spatial resolution satellite imagery can provide a better mechanistic interpretation of the distribution and sources of stress, despite the typical small size of fields (20-50 m scale). QuickBird imagery successfully detects stress within field and local scales, whereas SPOT HRV imagery is useful in detecting stress at a regional scale, and therefore, can be a robust tool in identifying issues of crop management at a regional scale. Due to the limited spectral capabilities of high spatial resolution images, distinguishing different sources of stress is not directly possible, and therefore, hyperspectral satellite imagery (e.g. Hyperion or HyspIRI) is required to distinguish between moisture and salinity induced stress. It is evident from the results that remotely sensed data acquired by both in situ hyperspectral and high spatial resolution satellite remote sensing can be used as a useful tool in precision farming in the Nile Valley, Egypt. A combined approach of using reliable high spatial and spectral satellite remote sensing data could provide better insight about stress at local and regional scales. Using this technique as a precision farming and management tool will lead to improved crop productivity by limiting stress and consequently provide a valuable tool in combating issues of food supply at a time of rapid population growth

Alkynyl N-BODIPYs as Reactive Intermediates for the Development of Dyes for Biophotonics

A new approach for the rapid multi-functionalization of BODIPY dyes towards biophotonics is reported. It is based on novel N-BODIPYs, through reactive intermediates with alkynyl groups to be further derivatized by click chemistry. This approach has been exemplified by the development of new dyes for cell bio-imaging, which have proven to successfully internalize into pancreatic cancer cells and accumulate in the mitochondria. The in vitro suitability for photodynamic therapy (PDT) was also analyzed and confirmed our compounds to be promising PDT candidates for the treatment of pancreatic cancer

Remote sensing of biotic stress in crop plants and its applications for pest management

Not AvailableRemote sensing (RS) of biotic stress is based on the assumption that stress interferes with photosynthesis and physical structure of the plant at tissue and canopy level, and thus affects the absorption of light energy and alters the refl ectance spectrum. Research into vegetative spectral refl ectance can help us gain a better understanding of the physical, physiological and chemical processes in plants due to pest and disease attack and to detect the resulting biotic stress. This has important implications to effective pest management. This review provides an overview of detection of various biotic stresses in different crops using various RS platforms. Previous work pertaining to the use of RS technique for assessing pest and disease severity using different RS techniques is briefl y summerized. The available sources of ground based, airborne and satellite sensors are presented along with various narrow band vegetation indices that could be used for characterizing biotic stress. Using relevant examples, the merits and demerits of various RS sensors and platforms for detection of pests and diseases are discussed. Pest surveillance programs such as fi eld scoutings are often expensive, time consuming, laborious and prone to error. As remote sensing gives a synoptic view of the area in a non-destructive and noninvasive way, this technology could be effective and provide timely information on spatial variability of pest damage over a large area. Thus remote sensing can guide scouting efforts and crop protection advisory in a more precise and effective manner. With the recent advancements in the communication, aviation and space technology, there is a lot of potential for application of remote sensing technology in the fi eld of pest management.Not Availabl

Leaf nitrogen determination using non-destructive techniques–A review

© 2017 Taylor & Francis Group, LLC. The optimisation of plant nitrogen-use-efficiency (NUE) has a direct impact on increasing crop production by optimising use of nitrogen fertiliser. Moreover, it protects environment from negative effects of nitrate leaching and nitrous oxide production. Accordingly, nitrogen (N) management in agriculture systems has been major focus of many researchers. Improvement of NUE can be achieved through several methods including more accurate measurement of foliar N contents of crops during different growth phases. There are two types of methods to diagnose foliar N status: destructive and non-destructive. Destructive methods are expensive and time-consuming, as they require tissue sampling and subsequent laboratory analysis. Thus, many farmers find destructive methods to be less attractive. Non-destructive methods are rapid and less expensive but are usually less accurate. Accordingly, improving the accuracy of non-destructive N estimations has become a common goal of many researchers, and various methods varying in complexity and optimality have been proposed for this purpose. This paper reviews various commonly used non-destructive methods for estimating foliar N status of plants

Remote Sensing for Precision Agriculture: Sentinel-2 Improved Features and Applications

The use of satellites to monitor crops and support their management is gathering increasing attention. The improved temporal, spatial, and spectral resolution of the European Space Agency (ESA) launched Sentinel-2 A + B twin platform is paving the way to their popularization in precision agriculture. Besides the Sentinel-2 A + B constellation technical features the open-access nature of the information they generate, and the available support software are a significant improvement for agricultural monitoring. This paper was motivated by the challenges faced by researchers and agrarian institutions entering this field; it aims to frame remote sensing principles and Sentinel-2 applications in agriculture. Thus, we reviewed the features and uses of Sentinel-2 in precision agriculture, including abiotic and biotic stress detection, and agricultural management. We also compared the panoply of satellites currently in use for land remote sensing that are relevant for agriculture to the Sentinel-2 A + B constellation features. Contrasted with previous satellite image systems, the Sentinel-2 A + B twin platform has dramatically increased the capabilities for agricultural monitoring and crop management worldwide. Regarding crop stress monitoring, Sentinel-2 capacities for abiotic and biotic stresses detection represent a great step forward in many ways though not without its limitations; therefore, combinations of field data and different remote sensing techniques may still be needed. We conclude that Sentinel-2 has a wide range of useful applications in agriculture, yet still with room for further improvements. Current and future ways that Sentinel-2 can be utilized are also discussed.This research was funded by the Spanish projects AGL2016-76527-R and IRUEC PCIN-2017-063 from the Ministerio de Economía y Competividad (MINECO, Spain) and by the support of Catalan Institution for Research and Advanced Studies (ICREA, Generalitat de Catalunya, Spain), through the ICREA Academia Program

High-throughput phenotyping of plant leaf morphological, physiological, and biochemical traits on multiple scales using optical sensing

Acquisition of plant phenotypic information facilitates plant breeding, sheds light on gene action, and can be applied to optimize the quality of agricultural and forestry products. Because leaves often show the fastest responses to external environmental stimuli, leaf phenotypic traits are indicators of plant growth, health, and stress levels. Combination of new imaging sensors, image processing, and data analytics permits measurement over the full life span of plants at high temporal resolution and at several organizational levels from organs to individual plants to field populations of plants. We review the optical sensors and associated data analytics used for measuring morphological, physiological, and biochemical traits of plant leaves on multiple scales. We summarize the characteristics, advantages and limitations of optical sensing and data-processing methods applied in various plant phenotyping scenarios. Finally, we discuss the future prospects of plant leaf phenotyping research. This review aims to help researchers choose appropriate optical sensors and data processing methods to acquire plant leaf phenotypes rapidly, accurately, and cost-effectively

Remote Sensing for Precision Agriculture: Sentinel-2 Improved Features and Applications

The use of satellites to monitor crops and support their management is gathering increasing attention. The improved temporal, spatial, and spectral resolution of the European Space Agency (ESA) launched Sentinel-2 A + B twin platform is paving the way to their popularization in precision agriculture. Besides the Sentinel-2 A + B constellation technical features the open-access nature of the information they generate, and the available support software are a significant improvement for agricultural monitoring. This paper was motivated by the challenges faced by researchers and agrarian institutions entering this field; it aims to frame remote sensing principles and Sentinel-2 applications in agriculture. Thus, we reviewed the features and uses of Sentinel-2 in precision agriculture, including abiotic and biotic stress detection, and agricultural management. We also compared the panoply of satellites currently in use for land remote sensing that are relevant for agriculture to the Sentinel-2 A + B constellation features. Contrasted with previous satellite image systems, the Sentinel-2 A + B twin platform has dramatically increased the capabilities for agricultural monitoring and crop management worldwide. Regarding crop stress monitoring, Sentinel-2 capacities for abiotic and biotic stresses detection represent a great step forward in many ways though not without its limitations; therefore, combinations of field data and different remote sensing techniques may still be needed. We conclude that Sentinel-2 has a wide range of useful applications in agriculture, yet still with room for further improvements. Current and future ways that Sentinel-2 can be utilized are also discusse