Foetal echocardiographic segmentation

Congenital heart disease affects just under one percentage of all live births [1]. Those defects that manifest themselves as changes to the cardiac chamber volumes are the motivation for the research presented in this thesis. Blood volume measurements in vivo require delineation of the cardiac chambers and manual tracing of foetal cardiac chambers is very time consuming and operator dependent. This thesis presents a multi region based level set snake deformable model applied in both 2D and 3D which can automatically adapt to some extent towards ultrasound noise such as attenuation, speckle and partial occlusion artefacts. The algorithm presented is named Mumford Shah Sarti Collision Detection (MSSCD). The level set methods presented in this thesis have an optional shape prior term for constraining the segmentation by a template registered to the image in the presence of shadowing and heavy noise. When applied to real data in the absence of the template the MSSCD algorithm is initialised from seed primitives placed at the centre of each cardiac chamber. The voxel statistics inside the chamber is determined before evolution. The MSSCD stops at open boundaries between two chambers as the two approaching level set fronts meet. This has significance when determining volumes for all cardiac compartments since cardiac indices assume that each chamber is treated in isolation. Comparison of the segmentation results from the implemented snakes including a previous level set method in the foetal cardiac literature show that in both 2D and 3D on both real and synthetic data, the MSSCD formulation is better suited to these types of data. All the algorithms tested in this thesis are within 2mm error to manually traced segmentation of the foetal cardiac datasets. This corresponds to less than 10% of the length of a foetal heart. In addition to comparison with manual tracings all the amorphous deformable model segmentations in this thesis are validated using a physical phantom. The volume estimation of the phantom by the MSSCD segmentation is to within 13% of the physically determined volume

Tools for developing continuous-flow micro-mixer : numerical simulation of transitional flow in micro geometries and a quantitative technique for extracting dynamic information from micro-bubble images

Recent advance in the microfluidics including its fabrication technologies has led to many novel applications in micro-scale flows. Among them is the continuous-flow micromixer that utilizes the advantages associated with turbulent flows for rapid mixing, achieving the detection of fast kinetic reaction as short as tens of microseconds. However, for developing a high performance continuous-flow micromixer there are certain fundamental issues need to be solved. One of them is an universal simulation approach capable of calculating the flow field across entire passage for entire regime from very low Reynolds number laminar flow through transition to fully turbulent flow. Though the direct numerical simulation is potentially possible solution but its extremely high computing time stops itself from practical applications. The second major issue is the inevitable occurrence of cavitation bubbles in this rapid flow apparatus. This phenomenon has opposite effects: (a) deteriorating performance and damaging the micromixer; (b) playing a catalyst role in enhancing mixing. A fully understanding of these micro bubbles will provide a sound theoretical base for guiding the design of micromixer in order to explore the advantage to maximum while minimizing its disadvantages. Therefore, the objectives of this PhD programme is to study the tools that will effectively advance our fundamental understandings on these key issues while in short term fulfil the requires from the joint experimental PhD programme held in the life science faculty for designing a prototype experimental device. During this PhD study, an existing numerical approach suitable for predicting the possibly entire flow regime including the turbulence transition is proposed for simulating the microscale flows in the microchannel and micromixer. The simulation results are validated against the transitional micro-channel experiments and this numerical method is then further applied for the micromixer simulation. This provides the researcher a realistic and feasible CFD tool to establish guidelines for designing high-efficiency and cost-effective micromixers by utilizing various possible measures which may cause very different flows simultaneously in micromixer. In order to study microscale cavitation bubbles and their effects on micromixers, an innovative experimental setup is purposely designed and constructed that can generate laser-induced micro-bubbles at desired position and size for testing. Experiments withvarious micro-scale bubbles have been performed successfully by using an ultra high-speed camera up to 1 million frame rate per second. A novel technique for tracking the contours of micro-scale cavitation bubble dynamically has been developed by using active contour method. By using this technique, for the first time, various geometric and dynamic data of cavitation bubble have been obtained to quantitatively analyze the global behaviours of bubbles thoroughly. This powerful tool will greatly benefit the study of bubble dynamics and similar demands in other fields for fast and accurate image treatments as well

Smart Cage Active Contours and their application to brain image segmentation

In this work we present a new segmentation method named Smart Cage Active Contours (SCAC) that combines a parametrized active contour framework named Cage Active Contours (CAC), based on a ne trans- formations, with Active Shape Models (ASM). Our method e ectively restricts the shapes the evolving contours can take without the need of the training images to be manually landmarked. We apply our method to segment the caudate nuclei subcortical structure of a set of 40 subjects in magnetic resonance brain images, with promising results

Segmentation in Echocardiographic Sequences Using Shape-based Snake Model

A method for segmentation of cardiac structures especially for mitral valve in echocardiographic sequences is presented. The method is motivated by the observation that the structures of neighboring frames have consistent locations and shapes that aid in segmentation. To cooperate with the constraining information provided by the neighboring frames, we combine the template matching with the conventional snake model. It means that the model not only is driven by conventional internal and external forces, but also combines an additional constraint, the matching degree to measure the similarity between the neighboring prior shape and the derived contour. Furthermore, in order to automatically or semi-automatically segment the sequent images without manually drawing the initial contours in each image, generalized Hough transformation (GHT) is used to roughly estimate the initial contour by transforming the neighboring prior shape. Based on the experiments on forty sequences, the method is particularly useful in case of the large frame-to-frame displacement of structure such as mitral valve. As a result, the active contour can easily detect the desirable boundaries in ultrasound images and has a high penetrability through the interference of various undesirables, such as the speckle, the tissue-related textures and the artifacts

Multi-scale active shape description in medical imaging

Shape description in medical imaging has become an increasingly important research field in recent years. Fast and high-resolution image acquisition methods like Magnetic Resonance (MR) imaging produce very detailed cross-sectional images of the human body - shape description is then a post-processing operation which abstracts quantitative descriptions of anatomically relevant object shapes. This task is usually performed by clinicians and other experts by first segmenting the shapes of interest, and then making volumetric and other quantitative measurements. High demand on expert time and inter- and intra-observer variability impose a clinical need of automating this process. Furthermore, recent studies in clinical neurology on the correspondence between disease status and degree of shape deformations necessitate the use of more sophisticated, higher-level shape description techniques. In this work a new hierarchical tool for shape description has been developed, combining two recently developed and powerful techniques in image processing: differential invariants in scale-space, and active contour models. This tool enables quantitative and qualitative shape studies at multiple levels of image detail, exploring the extra image scale degree of freedom. Using scale-space continuity, the global object shape can be detected at a coarse level of image detail, and finer shape characteristics can be found at higher levels of detail or scales. New methods for active shape evolution and focusing have been developed for the extraction of shapes at a large set of scales using an active contour model whose energy function is regularized with respect to scale and geometric differential image invariants. The resulting set of shapes is formulated as a multiscale shape stack which is analysed and described for each scale level with a large set of shape descriptors to obtain and analyse shape changes across scales. This shape stack leads naturally to several questions in regard to variable sampling and appropriate levels of detail to investigate an image. The relationship between active contour sampling precision and scale-space is addressed. After a thorough review of modem shape description, multi-scale image processing and active contour model techniques, the novel framework for multi-scale active shape description is presented and tested on synthetic images and medical images. An interesting result is the recovery of the fractal dimension of a known fractal boundary using this framework. Medical applications addressed are grey-matter deformations occurring for patients with epilepsy, spinal cord atrophy for patients with Multiple Sclerosis, and cortical impairment for neonates. Extensions to non-linear scale-spaces, comparisons to binary curve and curvature evolution schemes as well as other hierarchical shape descriptors are discussed

Multi-Surface Simplex Spine Segmentation for Spine Surgery Simulation and Planning

This research proposes to develop a knowledge-based multi-surface simplex deformable model for segmentation of healthy as well as pathological lumbar spine data. It aims to provide a more accurate and robust segmentation scheme for identification of intervertebral disc pathologies to assist with spine surgery planning. A robust technique that combines multi-surface and shape statistics-aware variants of the deformable simplex model is presented. Statistical shape variation within the dataset has been captured by application of principal component analysis and incorporated during the segmentation process to refine results. In the case where shape statistics hinder detection of the pathological region, user-assistance is allowed to disable the prior shape influence during deformation. Results have been validated against user-assisted expert segmentation