88,554 research outputs found
Efficient delay-tolerant particle filtering
This paper proposes a novel framework for delay-tolerant particle filtering
that is computationally efficient and has limited memory requirements. Within
this framework the informativeness of a delayed (out-of-sequence) measurement
(OOSM) is estimated using a lightweight procedure and uninformative
measurements are immediately discarded. The framework requires the
identification of a threshold that separates informative from uninformative;
this threshold selection task is formulated as a constrained optimization
problem, where the goal is to minimize tracking error whilst controlling the
computational requirements. We develop an algorithm that provides an
approximate solution for the optimization problem. Simulation experiments
provide an example where the proposed framework processes less than 40% of all
OOSMs with only a small reduction in tracking accuracy
Collaborative Spectrum Sensing from Sparse Observations in Cognitive Radio Networks
Spectrum sensing, which aims at detecting spectrum holes, is the precondition
for the implementation of cognitive radio (CR). Collaborative spectrum sensing
among the cognitive radio nodes is expected to improve the ability of checking
complete spectrum usage. Due to hardware limitations, each cognitive radio node
can only sense a relatively narrow band of radio spectrum. Consequently, the
available channel sensing information is far from being sufficient for
precisely recognizing the wide range of unoccupied channels. Aiming at breaking
this bottleneck, we propose to apply matrix completion and joint sparsity
recovery to reduce sensing and transmitting requirements and improve sensing
results. Specifically, equipped with a frequency selective filter, each
cognitive radio node senses linear combinations of multiple channel information
and reports them to the fusion center, where occupied channels are then decoded
from the reports by using novel matrix completion and joint sparsity recovery
algorithms. As a result, the number of reports sent from the CRs to the fusion
center is significantly reduced. We propose two decoding approaches, one based
on matrix completion and the other based on joint sparsity recovery, both of
which allow exact recovery from incomplete reports. The numerical results
validate the effectiveness and robustness of our approaches. In particular, in
small-scale networks, the matrix completion approach achieves exact channel
detection with a number of samples no more than 50% of the number of channels
in the network, while joint sparsity recovery achieves similar performance in
large-scale networks.Comment: 12 pages, 11 figure
Membrane proteins in the outer mebrane of plastids and mitochondria
Channels of the plastid and mitochondrial outer membranes facilitate the turnover of molecules and ions via these membranes. Although channels have been studied many questions pertaining to the whole diversity of plastid and mitochondrial channels in Arabidopsis thaliana and Pisum sativum remain unanswered. In this thesis I studied OEP16, OEP37 and VDAC families in two model plants, in Arabidopsis and pea.
The Arabidopsis OEP16 family represents four channels of α-helical structure, similar to the pea OEP16 protein. These channels are suggested to transport amino acids and compounds with primary amino groups. Immunoblot analysis, GFP/RFP protein fusion expression, as well as proteomic analysis showed that AtOEP16.1, AtOEP16.2 and AtOEP16.4 are located in the outer envelope membrane of plastids, while AtOEP16.3 is in mitochondria. The gene expression and immunoblot analyses revealed that AtOEP16.1 and AtOEP16.3 proteins are highly abundant and ubiquitous; expression of AtOEP16.1 is regulated by light and cold. AtOEP16.2 is highly expressed in pollen, seeds and seedlings. AtOEP16.4 is a low expressed housekeeping protein. Single knockout mutants of AtOEP16.1, AtOEP16.2 and AtOEP16.4, and double mutants of AtOEP16 gene family did not show any remarkable phenotype. However, macroarray analysis of Atoep16.1-p T-DNA mutant revealed 10 down-regulated and 6 up-regulated genes.
In contrast to the α-helical OEP16 proteins, the OEP37 and VDAC proteins are of β-barrel structure. The PsOEP37 and AtOEP37 channel proteins form a selective barrier in the outer envelope of chloroplasts. Electrophysiological studies in lipid bilayer membranes showed that the PsOEP37 channel is permeable for cations. Specific expression profiles showed that AtOEP37 and PsOEP37 are highly expressed in the entire plant.
The isolated PsVDAC gene encodes a protein, which is located in mitochondria. In Arabidopsis gene database, five Arabidopsis genes, which code for VDAC-like proteins were announced. One gene was not detected, whereas four of these genes expressed in leaves, roots, flower buds and pollen
Bibliographic Review on Distributed Kalman Filtering
In recent years, a compelling need has arisen to understand the effects of distributed information structures on estimation and filtering. In this paper, a bibliographical review on distributed Kalman filtering (DKF) is provided.\ud
The paper contains a classification of different approaches and methods involved to DKF. The applications of DKF are also discussed and explained separately. A comparison of different approaches is briefly carried out. Focuses on the contemporary research are also addressed with emphasis on the practical applications of the techniques. An exhaustive list of publications, linked directly or indirectly to DKF in the open literature, is compiled to provide an overall picture of different developing aspects of this area
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ER/K linked GPCR-G protein fusions systematically modulate second messenger response in cells.
FRET and BRET approaches are well established for detecting ligand induced GPCR-G protein interactions in cells. Currently, FRET/BRET assays rely on co-expression of GPCR and G protein, and hence depend on the stoichiometry and expression levels of the donor and acceptor probes. On the other hand, GPCR-G protein fusions have been used extensively to understand the selectivity of GPCR signaling pathways. However, the signaling properties of fusion proteins are not consistent across GPCRs. In this study, we describe and characterize novel sensors based on the Systematic Protein Affinity Strength Modulation (SPASM) technique. Sensors consist of a GPCR and G protein tethered by an ER/K linker flanked by FRET probes. SPASM sensors are tested for the β2-, α1-, and α2- adrenergic receptors, and adenosine type 1 receptor (A1R), tethered to Gαs-XL, Gαi2, or Gαq subunits. Agonist stimulation of β2-AR and α2-AR increases FRET signal comparable to co-expressed FRET/BRET sensors. SPASM sensors also retain signaling through the endogenous G protein milieu. Importantly, ER/K linker length systematically tunes the GPCR-G protein interaction, with consequent modulation of second messenger signaling for cognate interactions. SPASM GPCR sensors serve the dual purpose of detecting agonist-induced changes in GPCR-G protein interactions, and linking these changes to downstream signaling
Identification and functional characterization of an N-terminal oligomerization domain for polycystin-2*
Autosomal dominant polycystic kidney disease (ADPKD), the most common inherited cause of kidney failure, is caused by mutations in either PKD1 (85%) or PKD2 (15%). The PKD2 protein, polycystin-2 (PC2 or TRPP2), is a member of the transient receptor potential (TRP) superfamily and functions as a non-selective calcium channel. PC2 has been found to form oligomers in native tissues suggesting that it may form functional homo- or heterotetramers with other subunits, similar to other TRP channels. Our experiments unexpectedly revealed that PC2 mutant proteins lacking the known C-terminal dimerization domain were still able to form oligomers and co-immunoprecipitate full-length PC2, implying the possible existence of a proximal dimerization domain. Using yeast two-hybrid and biochemical assays, we have mapped an alternative dimerization domain to the N terminus of PC2 (NT2-1-223, L224X). Functional characterization of this domain demonstrated that it was sufficient to induce cyst formation in zebrafish embryos and inhibit PC2 surface currents in mIMCD3 cells probably by a dominant-negative mechanism. In summary, we propose a model for PC2 assembly as a functional tetramer which depends on both C- and N-terminal dimerization domains. These results have significant implications for our understanding of PC2 function and disease pathogenesis in ADPKD and provide a new strategy for studying PC2 function
Very Rare Complementation between Mitochondria Carrying Different Mitochondrial DNA Mutations Points to Intrinsic Genetic Autonomy of the Organelles in Cultured Human Cells
In the present work, a large scale investigation was done regarding the capacity of cultured human cell lines (carrying in homoplasmic form either the mitochondrial tRNALys A8344G mutation associated with the myoclonic epilepsy and ragged red fiber (MERRF) encephalomyopathy or a frameshift mutation, isolated in vitro, in the gene for the ND4 subunit of NADH dehydrogenase) to undergo transcomplementation of their recessive mitochondrial DNA (mtDNA) mutations after cell fusion. The presence of appropriate nuclear drug resistance markers in the two cell lines allowed measurements of the frequency of cell fusion in glucose-containing medium, non-selective for respiratory capacity, whereas the frequency of transcomplementation of the two mtDNA mutations was determined by growing the same cell fusion mixture in galactose-containing medium, selective for respiratory competence. Transcomplementation of the two mutations was revealed by the re-establishment of normal mitochondrial protein synthesis and respiratory activity and by the relative rates synthesis of two isoforms of the ND3 subunit of NADH dehydrogenase. The results of several experiments showed a cell fusion frequency between 1.4 and 3.4% and an absolute transcomplementation frequency that varied between 1.2 Ă— 10^-5 and 5.5 Ă— 10^-4. Thus, only 0.3-1.6% of the fusion products exhibited transcomplementation of the two mutations. These rare transcomplementing clones were very sluggish in developing, grew very slowly thereafter, and showed a substantial rate of cell death (22-28%). The present results strongly support the conclusion that the capacity of mitochondria to fuse and mix their contents is not a general intrinsic property of these organelles in mammalian cells, although it may become activated in some developmental or physiological situations
Numerical modelling of heat transfer and experimental validation in Powder-Bed Fusion with the Virtual Domain Approximation
Among metal additive manufacturing technologies, powder-bed fusion features
very thin layers and rapid solidification rates, leading to long build jobs and
a highly localized process. Many efforts are being devoted to accelerate
simulation times for practical industrial applications. The new approach
suggested here, the virtual domain approximation, is a physics-based rationale
for spatial reduction of the domain in the thermal finite-element analysis at
the part scale. Computational experiments address, among others, validation
against a large physical experiment of 17.5 of deposited
volume in 647 layers. For fast and automatic parameter estimation at such level
of complexity, a high-performance computing framework is employed. It couples
FEMPAR-AM, a specialized parallel finite-element software, with Dakota, for the
parametric exploration. Compared to previous state-of-the-art, this formulation
provides higher accuracy at the same computational cost. This sets the path to
a fully virtualized model, considering an upwards-moving domain covering the
last printed layers
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