133,732 research outputs found
PLoS Negl Trop Dis
BackgroundSchistosomiasis japonica is a serious debilitating and sometimes fatal disease. Accurate diagnostic tests play a key role in patient management and control of the disease. However, currently available diagnostic methods are not ideal, and the detection of the parasite DNA in blood samples has turned out to be one of the most promising tools for the diagnosis of schistosomiasis. In our previous investigations, a 230-bp sequence from the highly repetitive retrotransposon SjR2 was identified and it showed high sensitivity and specificity for detecting Schistosoma japonicum DNA in the sera of rabbit model and patients. Recently, 29 retrotransposons were found in S. japonicum genome by our group. The present study highlighted the key factors for selecting a new perspective sensitive target DNA sequence for the diagnosis of schistosomiasis, which can serve as example for other parasitic pathogens.Methodology/Principal FindingsIn this study, we demonstrated that the key factors based on the bioinformatic analysis for selecting target sequence are the higher genome proportion, repetitive complete copies and partial copies, and active ESTs than the others in the chromosome genome. New primers based on 25 novel retrotransposons and SjR2 were designed and their sensitivity and specificity for detecting S. japonicum DNA were compared. The results showed that a new 303-bp sequence from non-long terminal repeat (LTR) retrotransposon (SjCHGCS19) had high sensitivity and specificity. The 303-bp target sequence was amplified from the sera of rabbit model at 3 d post-infection by nested-PCR and it became negative at 17 weeks post-treatment. Furthermore, the percentage sensitivity of the nested-PCR was 97.67% in 43 serum samples of S. japonicum-infected patients.Conclusions/SignificanceOur findings highlighted the key factors based on the bioinformatic analysis for selecting target sequence from S. japonicum genome, which provide basis for establishing powerful molecular diagnostic techniques that can be used for monitoring early infection and therapy efficacy to support schistosomiasis control programs
SAFS: A Deep Feature Selection Approach for Precision Medicine
In this paper, we propose a new deep feature selection method based on deep
architecture. Our method uses stacked auto-encoders for feature representation
in higher-level abstraction. We developed and applied a novel feature learning
approach to a specific precision medicine problem, which focuses on assessing
and prioritizing risk factors for hypertension (HTN) in a vulnerable
demographic subgroup (African-American). Our approach is to use deep learning
to identify significant risk factors affecting left ventricular mass indexed to
body surface area (LVMI) as an indicator of heart damage risk. The results show
that our feature learning and representation approach leads to better results
in comparison with others
CBR and MBR techniques: review for an application in the emergencies domain
The purpose of this document is to provide an in-depth analysis of current reasoning engine practice and the integration strategies of Case Based Reasoning and Model Based Reasoning that will be used in the design and development of the RIMSAT system.
RIMSAT (Remote Intelligent Management Support and Training) is a European Commission funded project designed to:
a.. Provide an innovative, 'intelligent', knowledge based solution aimed at improving the quality of critical decisions
b.. Enhance the competencies and responsiveness of individuals and organisations involved in highly complex, safety critical incidents - irrespective of their location.
In other words, RIMSAT aims to design and implement a decision support system that using Case Base Reasoning as well as Model Base Reasoning technology is applied in the management of emergency situations.
This document is part of a deliverable for RIMSAT project, and although it has been done in close contact with the requirements of the project, it provides an overview wide enough for providing a state of the art in integration strategies between CBR and MBR technologies.Postprint (published version
Intelligent systems in manufacturing: current developments and future prospects
Global competition and rapidly changing customer requirements are demanding increasing changes in manufacturing environments. Enterprises are required to constantly redesign their products and continuously reconfigure their manufacturing systems. Traditional approaches to manufacturing systems do not fully satisfy this new situation. Many authors have proposed that artificial intelligence will bring the flexibility and efficiency needed by manufacturing systems. This paper is a review of artificial intelligence techniques used in manufacturing systems. The paper first defines the components of a simplified intelligent manufacturing systems (IMS), the different Artificial Intelligence (AI) techniques to be considered and then shows how these AI techniques are used for the components of IMS
A Pattern Language for High-Performance Computing Resilience
High-performance computing systems (HPC) provide powerful capabilities for
modeling, simulation, and data analytics for a broad class of computational
problems. They enable extreme performance of the order of quadrillion
floating-point arithmetic calculations per second by aggregating the power of
millions of compute, memory, networking and storage components. With the
rapidly growing scale and complexity of HPC systems for achieving even greater
performance, ensuring their reliable operation in the face of system
degradations and failures is a critical challenge. System fault events often
lead the scientific applications to produce incorrect results, or may even
cause their untimely termination. The sheer number of components in modern
extreme-scale HPC systems and the complex interactions and dependencies among
the hardware and software components, the applications, and the physical
environment makes the design of practical solutions that support fault
resilience a complex undertaking. To manage this complexity, we developed a
methodology for designing HPC resilience solutions using design patterns. We
codified the well-known techniques for handling faults, errors and failures
that have been devised, applied and improved upon over the past three decades
in the form of design patterns. In this paper, we present a pattern language to
enable a structured approach to the development of HPC resilience solutions.
The pattern language reveals the relations among the resilience patterns and
provides the means to explore alternative techniques for handling a specific
fault model that may have different efficiency and complexity characteristics.
Using the pattern language enables the design and implementation of
comprehensive resilience solutions as a set of interconnected resilience
patterns that can be instantiated across layers of the system stack.Comment: Proceedings of the 22nd European Conference on Pattern Languages of
Program
Applying tropos to socio-technical system design and runtime configuration
Recent trends in Software Engineering have introduced the importance of reconsidering the traditional idea of software design as a socio-tecnical problem, where human agents are integral part of the system along with hardware and software components. Design and runtime support for Socio-Technical Systems (STSs) requires appropriate modeling techniques and
non-traditional infrastructures. Agent-oriented software methodologies are natural solutions to the development of STSs, both humans and technical components are conceptualized and analyzed as part of the same system. In this paper, we illustrate a number of Tropos features that we believe fundamental to support the development and runtime reconfiguration of STSs.
Particularly, we focus on two critical design issues: risk analysis and location variability. We show how they are integrated and used into a planning-based approach to support the designer in evaluating and choosing the best design alternative. Finally, we present a generic framework to develop self-reconfigurable STSs
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