The optimal synthesis of scanned linear antenna arrays

In this paper, symmetric scanned linear antenna arrays are synthesized, in order to minimize the side lobe level of the radiation pattern. The feeding current amplitudes are considered as the optimization parameters. Newly proposed optimization algorithms are presented to achieve our target; Antlion Optimization (ALO) and a new hybrid algorithm. Three different examples are illustrated in this paper; 20, 26 and 30 elements scanned linear antenna array. The obtained results prove the effectiveness and the ability of the proposed algorithms to outperform and compete other algorithms like Symbiotic Organisms Search (SOS) and Firefly Algorithm (FA)

Transcription start site scanning and the requirement for ATP during transcription initiation by RNA polymerase II

Saccharomyces cerevisiae RNA polymerase (Pol) II locates transcription start sites (TSS) at TATA-containing promoters by scanning sequences downstream from the site of preinitiation complex formation, a process that involves the translocation of downstream promoter DNA toward Pol II. To investigate a potential role of yeast Pol II transcription in TSS scanning, HIS4 promoter derivatives were generated that limited transcripts in the 30-bp scanned region to two nucleotides in length. Although we found that TSS scanning does not require RNA synthesis, our results revealed that transcription in the purified yeast basal system is largely ATP-independent despite a requirement for the TFIIH DNA translocase subunit Ssl2. This result is rationalized by our finding that, although they are poorer substrates, UTP and GTP can also be utilized by Ssl2. ATPγS is a strong inhibitor of rNTP-fueled translocation, and high concentrations of ATPγS make transcription completely dependent on added dATP. Limiting Pol II function with low ATP concentrations shifted the TSS position downstream. Combined with prior work, our results show that Pol II transcription plays an important role in TSS selection but is not required for the scanning reaction

Tester versus Bug: A Generic Framework for Model-Based Testing via Games

We propose a generic game-based approach for test case generation. We set up a game between the tester and the System Under Test, in such a way that test cases correspond to game strategies, and the conformance relation ioco corresponds to alternating refinement. We show that different test assumptions from the literature can be easily incorporated, by slightly varying the moves in the games and their outcomes. In this way, our framework allows a wide plethora of game-theoretic techniques to be deployed for model based testing.Comment: In Proceedings GandALF 2018, arXiv:1809.0241

Shape Completion using 3D-Encoder-Predictor CNNs and Shape Synthesis

We introduce a data-driven approach to complete partial 3D shapes through a combination of volumetric deep neural networks and 3D shape synthesis. From a partially-scanned input shape, our method first infers a low-resolution -- but complete -- output. To this end, we introduce a 3D-Encoder-Predictor Network (3D-EPN) which is composed of 3D convolutional layers. The network is trained to predict and fill in missing data, and operates on an implicit surface representation that encodes both known and unknown space. This allows us to predict global structure in unknown areas at high accuracy. We then correlate these intermediary results with 3D geometry from a shape database at test time. In a final pass, we propose a patch-based 3D shape synthesis method that imposes the 3D geometry from these retrieved shapes as constraints on the coarsely-completed mesh. This synthesis process enables us to reconstruct fine-scale detail and generate high-resolution output while respecting the global mesh structure obtained by the 3D-EPN. Although our 3D-EPN outperforms state-of-the-art completion method, the main contribution in our work lies in the combination of a data-driven shape predictor and analytic 3D shape synthesis. In our results, we show extensive evaluations on a newly-introduced shape completion benchmark for both real-world and synthetic data

A spatial analysis of physiological changes associated with infection of cotyledons of marrow plants with cucumber mosaic virus

Changes in host primary metabolism associated with the compatible interaction between cucumber mosaic virus and cotyledons of the marrow plant (Cucurbita pepo L.) have been localized, first by measuring activities of key enzymes in infected and uninfected regions of the cotyledon, and second by histochemical techniques applied to tissue prints of the infected region. A series of progressive metabolic changes occurs within the expanding infected lesion. Virus replication and the synthesis of viral protein at the periphery creates a strong sink demand associated with increased activities of anaplerotic enzymes, increased photosynthesis, and starch accumulation. Inside the lesion, when the synthesis of virus has declined, photosynthesis is reduced, starch is mobilized, and the emphasis of metabolism is shifted toward glycolysis and mitochondrial respiration. These changes are associated spatially with the onset of chlorosis. A decrease in total protein synthesis in this inner zone could be instrumental in some or all of these changes, leading to symptoms of viral infection

Revisiting the theory of interferometric wide-field synthesis

After several generations of interferometers in radioastronomy, wide-field imaging at high angular resolution is today a major goal for trying to match optical wide-field performances. All the radio-interferometric, wide-field imaging methods currently belong to the mosaicking family. Based on a 30 years old, original idea from Ekers & Rots, we aim at proposing an alternate formalism. Starting from their ideal case, we successively evaluate the impact of the standard ingredients of interferometric imaging. A comparison with standard nonlinear mosaicking shows that both processing schemes are not mathematically equivalent, though they both recover the sky brightness. In particular, the weighting scheme is very different in both methods. Moreover, the proposed scheme naturally processes the short spacings from both single-dish antennas and heterogeneous arrays. Finally, the sky gridding of the measured visibilities, required by the proposed scheme, may potentially save large amounts of hard-disk space and cpu processing power over mosaicking when handling data sets acquired with the on-the-fly observing mode. We propose to call this promising family of imaging methods wide-field synthesis because it explicitly synthesizes visibilities at a much finer spatial frequency resolution than the one set by the diameter of the interferometer antennas.Comment: 22 pages, 6 PostScript figures. Accepted for publication in Astronomy & Astrophysics. Uses aa LaTeX macros

Biocompatible Copper Oxide Nanoparticle Composites from Cellulose and Chitosan: Facile Synthesis, Unique Structure, and Antimicrobial Activity

Copper in various forms has been known to have bactericidal activity. Challenges to its application include preventing mobilization of the copper, to both extend activity and avoid toxicity, and bioincompatibility of many candidate substrates for copper immobilization. Using a simple ionic liquid, butylmethylimmidazolium chloride as the solvent, we developed a facile and green method to synthesize biocompatible composites containing copper oxide nanoparticles (CuONPs) from cellulose (CEL) and chitosan (CS) or CEL and keratin (KER). Spectroscopy and imaging results indicate that CEL, CS, and KER remained chemically intact and were homogeneously distributed in the composites with CuONPs with size of 22 ± 1 nm. Electron paramagnetic resonance (EPR) suggests that some 25% of the EPR-detectable Cu(II) is present as a monomeric species, chemically anchored to the substrate by two or more nitrogen atoms, and, further, adopts a unique spatially oriented conformation when incorporated into the [CEL + CS] composite but not in the [CEL + KER] composite. The remaining 75% of EPR-detectable Cu(II) exhibited extensive spin–spin interactions, consistent with Cu(II) aggregates and Cu(II) on the surface of CuONPs. At higher levels of added copper (\u3e59 nmol/mg), the additional copper was EPR-silent, suggesting an additional phase in larger CuONPs, in which S \u3e 0 spin states are either thermally inaccessible or very fast-relaxing. These data suggest that Cu(II) initially binds substrate via nitrogen atoms, from which CuONPs develop through aggregation of copper. The composites exhibited excellent antimicrobial activity against a wide range of bacteria and fungi, including methicillin-resistant Staphylococcus aureus; vancomycin-resistant Enterococcus; and highly resistant Escherichia coli, Streptococcus agalactiae, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Candida albicans. Expectedly, the antibacterial activity was found to be correlated with the CuONPs content in the composites. More importantly, at CuONP concentration of 35 nmol/mg or lower, bactericidal activity of the composite was complemented by its biocompatibility with human fibroblasts

Synthesis and systematic study of Co₃O₄-based catalysts for oxygen reduction and oxygen evolution reactions

textCo₃O₄-based composite materials are good electrocatalysts for the oxygen reduction reaction (ORR) and the oxygen evolution reaction (OER) in alkaline solutions. Here, this thesis first investigated the individual functionality of Co₃O₄ and the N-doped carbon nanoweb (CNW) in ORR and OER. The Co₃O₄/CNW bifunctional catalysts were synthesized by an in situ growth of Co precursors onto CNW followed by a controlled heat treatment. Rotating disk electrode measurements were utilized to provide insight into the specific functions of Co₃O₄ and CNW in the composite material during catalysis. It was found that Co₃O₄ alone exhibited poor ORR catalytic activity. However, in the presence of CNW, Co₃O₄ assisted the selective four-electron oxygen reduction over the two-electron pathway. Co₃O₄ acted as the primary catalytic site for OER and CNW improved the electronic conduction between Co₃O₄ and the current collector. CNW underwent serious degradation at the high potential of the OER, but its stability improved greatly upon the deposition of Co₃O₄. Two possible mechanisms for the improved catalytic stability are discussed. The findings demonstrate the specific functions of Co₃O₄ and CNW in catalyzing the OER and ORR and further establish an understanding of the synergy of the composite in electrocatalysis. Based on the critical functionality of Co₃O₄ in stabilizing carbon materials in the OER potential region, it is of interest to investigate novel synthesis methods to prepare nano-sized Co₃O₄ that can provide more active sites for catalytic reactions and thus, improve the OER kinetics. Here, in situ electrochemical generation of 2-dimensional Co₃O₄ (2D-Co₃O₄) nanoplates were achieved by scanning CoO[subscript x]/Co precursors in 1 M KOH solution. X-ray diffraction characterization suggested that CoO[subscript x]/Co precursors were oxidized to Co₃O₄ before the onset potential of OER. Scanning electron microscopy showed that oxidation from CoO[subscript x]/Co to 2D-Co₃O₄ was associated with the formation of hexagonal nanoplates. The 2D-Co₃O₄ exhibited excellent OER catalytic activity and stability probably due to the effective mass transfer through the 2D structure.Materials Science and Engineerin