Surveillance strategies for Classical Swine Fever in wild boar – a comprehensive evaluation study to ensure powerful surveillance

Surveillance of Classical Swine Fever (CSF) should not only focus on livestock, but must also include wild boar. To prevent disease transmission into commercial pig herds, it is therefore vital to have knowledge about the disease status in wild boar. In the present study, we performed a comprehensive evaluation of alternative surveillance strategies for Classical Swine Fever (CSF) in wild boar and compared them with the currently implemented conventional approach. The evaluation protocol was designed using the EVA tool, a decision support tool to help in the development of an economic and epidemiological evaluation protocol for surveillance. To evaluate the effectiveness of the surveillance strategies, we investigated their sensitivity and timeliness. Acceptability was analysed and finally, the cost-effectiveness of the surveillance strategies was determined. We developed 69 surveillance strategies for comparative evaluation between the existing approach and the novel proposed strategies. Sampling only within sub-adults resulted in a better acceptability and timeliness than the currently implemented strategy. Strategies that were completely based on passive surveillance performance did not achieve the desired detection probability of 95%. In conclusion, the results of the study suggest that risk-based approaches can be an option to design more effective CSF surveillance strategies in wild boar

How often should we monitor for reliable detection of atrial fibrillation recurrence? Efficiency considerations and implications for study design

OBJECTIVE: Although atrial fibrillation (AF) recurrence is unpredictable in terms of onset and duration, current intermittent rhythm monitoring (IRM) diagnostic modalities are short-termed and discontinuous. The aim of the present study was to investigate the necessary IRM frequency required to reliably detect recurrence of various AF recurrence patterns. METHODS: The rhythm histories of 647 patients (mean AF burden: 12±22% of monitored time; 687 patient-years) with implantable continuous monitoring devices were reconstructed and analyzed. With the use of computationally intensive simulation, we evaluated the necessary IRM frequency to reliably detect AF recurrence of various AF phenotypes using IRM of various durations. RESULTS: The IRM frequency required for reliable AF detection depends on the amount and temporal aggregation of the AF recurrence (p<0.0001) as well as the duration of the IRM (p<0.001). Reliable detection (>95% sensitivity) of AF recurrence required higher IRM frequencies (>12 24-hour; >6 7-day; >4 14-day; >3 30-day IRM per year; p<0.0001) than currently recommended. Lower IRM frequencies will under-detect AF recurrence and introduce significant bias in the evaluation of therapeutic interventions. More frequent but of shorter duration, IRMs (24-hour) are significantly more time effective (sensitivity per monitored time) than a fewer number of longer IRM durations (p<0.0001). CONCLUSIONS: Reliable AF recurrence detection requires higher IRM frequencies than currently recommended. Current IRM frequency recommendations will fail to diagnose a significant proportion of patients. Shorter duration but more frequent IRM strategies are significantly more efficient than longer IRM durations. CLINICAL TRIAL REGISTRATION URL: Unique identifier: NCT00806689

Sensitivity analysis of expensive black-box systems using metamodeling

Simulations are becoming ever more common as a tool for designing complex products. Sensitivity analysis techniques can be applied to these simulations to gain insight, or to reduce the complexity of the problem at hand. However, these simulators are often expensive to evaluate and sensitivity analysis typically requires a large amount of evaluations. Metamodeling has been successfully applied in the past to reduce the amount of required evaluations for design tasks such as optimization and design space exploration. In this paper, we propose a novel sensitivity analysis algorithm for variance and derivative based indices using sequential sampling and metamodeling. Several stopping criteria are proposed and investigated to keep the total number of evaluations minimal. The results show that both variance and derivative based techniques can be accurately computed with a minimal amount of evaluations using fast metamodels and FLOLA-Voronoi or density sequential sampling algorithms.Comment: proceedings of winter simulation conference 201

Data assimilation of in situ soil moisture measurements in hydrological models: first annual doctoral progress report, work plan and achievements

Water scarcity and the presence of water of good quality is a serious public concern since it determines the availability of water to society. Water scarcity especially in arid climates and due to extreme droughts related to climate change drive water use technologies such as irrigation to become more efficient and sustainable. Plant root water and nutrient uptake is one of the most important processes in subsurface unsaturated flow and transport modeling, as root uptake controls actual plant evapotranspiration, water recharge and nutrient leaching to the groundwater, and exerts a major influence on predictions of global climate models. To improve irrigation strategies, water flow needs to be accurately described using advanced monitoring and modeling. Our study focuses on the assimilation of hydrological data in hydrological models that predict water flow and solute (pollutants and salts) transport and water redistribution in agricultural soils under irrigation. Field plots of a potato farmer in a sandy region in Belgium were instrumented to continuously monitor soil moisture and water potential before, during and after irrigation in dry summer periods. The aim is to optimize the irrigation process by assimilating online sensor field data into process based models. Over the past year, we demonstrated the calibration and optimization of the Hydrus 1D model for an irrigated grassland on sandy soil. Direct and inverse calibration and optimization for both heterogeneous and homogeneous conceptualizations was applied. Results show that Hydrus 1D closely simulated soil water content at five depths as compared to water content measurements from soil moisture probes, by stepwise calibration and local sensivity analysis and optimization the Ks, n and α value in the calibration and optimization analysis. The errors of the model, expressed by deviations between observed and modeled soil water content were, however, different for each individual depth. The smallest differences between the observed value and soil-water content were attained when using an automated inverse optimization method. The choice of the initial parameter value can be optimized using a stepwise approach. Our results show that statistical evaluation coefficients (R2, Ce and RMSE) are suitable benchmarks to evaluate the performance of the model in reproducing the data. The degree of water stress simulated with Hydrus 1D suggested to increase irrigation at least one time, i.e. at the beginning of the simulation period and further distribute the amount of irrigation during the growing season, instead of using a huge amount of irrigation later in the season. In the next year, we will further look for to the best method (using soft data and methods for instance PTFs, EMI, Penetrometer) to derive and predict the spatial variability of soil hydraulic properties (saturated hydraulic conductivity) of the soil and link to crop yield at the field scale. Linear and non-linear pedotransfer functions (PTFs) have been assessed to predict penetrometer resistance of soils from their water status (matric potential, ψ and degree of saturation, S) and bulk density, ρb, and some other soil properties such as sand content, Ks etc. The geophysical EMI (electromagnetic induction) technique provides a versatile and robust field instrument for determining apparent soil electrical conductivity (ECa). ECa, a quick and reliable measurement, is one of ancillary properties (secondary information) of soil, can improve the spatial and temporal estimation of soil characteristics e.g., salinity, water content, texture, prosity and bulk density at different scales and depths. According to previous literature on penetrometer measurements, we determined the effective stress and used some models to find the relationships between soil properties, especially Ks, and penetrometer resistance as one of the prediction methods for Ks. The initial results obtained in the first yearshowed that a new data set would be necessary to validate the results of this part. In the third year, quasi 3D-modelling of water flow at the field scale will be conducted. In this modeling set -up, the field will be modeled as a collection of 1D-columns representing the different field conditions (combination of soil properties, groundwater depth, root zone depth). The measured soil properties are extrapolated over the entire field by linking them to the available spatially distributed data (such as the EMI-images). The data set of predicted Ks and other soil properties for the whole field constructed in the previous steps will be used for parameterising the model. Sensitivity analysis ‘SA’ is essential to the model optimization or parametrization process. To avoid overparameterization, the use of global sensitivity analysis (SA) will be investigated. In order to include multiple objectives (irrigation management parameters, costs, …) in the parameter optimization strategy, multi-objective techniques such as AMALGAM have been introduced. We will investigate multi-objective strategies in the irrigation optimization

An improved mosquito electrocuting trap that safely reproduces epidemiologically relevant metrics of mosquito human-feeding behaviours as determined by human landing catch

Background: Reliable quantification of mosquito host—seeking behaviours is required to determine the efficacy of vector control methods. For malaria, the gold standard approach remains the risky human landing catch (HLC). Here compare the performance of an improved prototype of the mosquito electrocuting grid trap (MET) as a safer alternative with HLC for measuring malaria vector behaviour in Dar es Salaam, Tanzania. Methods: Mosquito trapping was conducted at three sites within Dar es Salaam representing a range of urbanicity over a 7-month period (December 2012–July 2013, 168 sampling nights). At each site, sampling was conducted in a block of four houses, with two houses being allocated to HLC and the other to MET on each night of study. Sampling was conducted both indoors and outdoors (from 19:00 to 06:00 each night) at all houses, with trapping method (HLC and MET) being exchanged between pairs of houses at each site using a crossover design. Results: The MET caught significantly more Anopheles gambiae sensu lato than the HLC, both indoors (RR [95 % confidence interval (CI)]) = 1.47 [1.23–1.76], P &lt; 0.0001 and outdoors = 1.38 [1.14–1.67], P &lt; 0.0001). The sensitivity of MET compared with HLC did not detectably change over the course of night for either An. gambiae s.l. (OR [CI]) = 1.01 [0.94–1.02], P = 0.27) or Culex spp. (OR [CI]) = 0.99 [0.99–1.0], P = 0.17) indoors and declined only slightly outdoors: An. gambiae s.l. (OR [CI]) = 0.92 [0.86–0.99], P = 0.04), and Culex spp. (OR [CI]) = 0.99 [0.98–0.99], P = 0.03). MET-based estimates of the proportions of mosquitoes caught indoors (P i ) or during sleeping hours (P fl ), as well as the proportion of human exposure to bites that would otherwise occurs indoors (π i ), were statistically indistinguishable from those based on HLC for An. gambiae s.l. (P = 0.43, 0.07 and 0.48, respectively) and Culex spp. (P = 0.76, 0.24 and 0.55, respectively). Conclusions: This improved MET prototype is highly sensitive tool that accurately quantifies epidemiologically-relevant metrics of mosquito biting densities, behaviours and human exposure distribution