Advanced optical microscopies for materials: new trends

Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166This article summarizes the new trends of Optical Microscopy applied to Materials, with examples of applications that illustrate the capabilities of the technique

Automatic Recognition of Light Microscope Pollen Images

This paper is a progress report on a project aimed at the realization of a low-cost, automatic, trainable system "AutoStage" for recognition and counting of pollen. Previous work on image feature selection and classification has been extended by design and integration of an XY stage to allow slides to be scanned, an auto focus system, and segmentation software. The results of a series of classification tests are reported, and verified by comparison with classification performance by expert palynologists. A number of technical issues are addressed, including pollen slide preparation and slide sampling protocols

Exploiting multimode waveguides for pure fibre-based imaging

We acknowledge support from the UK Engineering and Physical Science Research CouncilThere has been an immense drive in modern microscopy towards miniaturisation and fibre based technology. This has been necessitated by the need to access hostile or diffcult environments in-situ and in-vivo. Strategies to date have included the use of specialist fibres and miniaturised scanning systems accompanied by ingenious microfabricated lenses. We present a novel approach for this field by utilising disordered light within a standard multimode optical fibre for lensless microscopy and optical mode conversion. We demonstrate the modalities of bright-field and dark-field imaging and scanning fluorescence microscopy at acquisition rates allowing observation of dynamic processes such as Brownian motion of mesoscopic particles. Furthermore, we show how such control can realise a new form of mode converter and generate various types of advanced light fields such as propagation-invariant beams and optical vortices. These may be useful for future fibre based implementations of super-resolution or light sheet microscopy.Publisher PDFPeer reviewe