Endophytic Fungi of Bitter Melon \u3ci\u3e(Momordica Charantia)\u3c/i\u3e in Guangdong Province, China

Endophytic fungi can mutualistically interact with their host plants by deterring herbivores. Overall 1172 endophytic fungal isolates were recovered from roots, stems, leaves, flowers and fruits of bitter melon, Momordica charantia, at five sites in Guangdong Province. These isolates were identified to 25 genera using morphological and molecular characteristics. The endophyte communities at the five sites were similar. Alternaria alternata, Aspergillus spp., Cladosporium spp., Colletotrichum spp., Nigrospora spp., Penicillium spp., Arthrinium spp., Chaetimium spp., Curvularia spp., Fusarium spp., Phoma spp., and Phomopsis spp. were isolated from at least three of the five sites. The coefficient of similarity for endophytes ranged from 60.6% to 83.3% between any two sites. There were significant differences in the species composition of endophytes recovered from different tissues of bitter melon. Fusarium spp. was the most frequent in root and stem samples, Colletotrichum spp. in leaf samples, A. alternata in flower samples, and Cladosporium spp. in fruit samples. The coefficients of similarity for endophytes were between 42.9% and 80.0% from any two tissues. We found that the composition of endophytes of bitter melon was relatively stable across sites, but differed greatly among tissues. We also found that there were fewer insects such as aphids (Homoptera: Aphididae), leafminers (Lepidoptera, Gracillariidae), and cotton leafworms Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) collected from the leaves of bitter melon at the Huadu site compared to those collected at the Yunfu site. Whether this is related to the endophyte communities isolated from different sites requires further research

Descriptions of new genera and species of South African spiders

The present paper contains descriptions of 4 new genera and 62 new species of South African Spiders contained in the Collection of the South African Museum. With the exception of 3 new species of Prodidomidae, all of them belong to the groups Mygalomorphae (Migidce, 1 n. sp.; Ctenizidae, 1 n. gen. and 5 n. spp.; Barychelidae, 2 n. spp.), Cribellatae (Uloboridae, 1 n. gen. and 3 n. spp.; Dictynirlce, 4 n. spp.; Eresidae, 6 n. spp.) , and Ecribellatae Haplogynae (Sicariidae, 19 n. spp.; Dysderidae, 1 n. geu. and 11 n. spp.; Caponiidae, 1 n. gen. and 8 n. spp.)

Efficient unidirectional nanoslit couplers for surface plasmons

Plasmonics is based on surface plasmon polariton (SPP) modes which can be laterally confined below the diffraction limit, thereby enabling ultracompact optical components. In order to exploit this potential, the fundamental bottleneck of poor light-SPP coupling must be overcome. In established SPP sources (using prism, grating} or nanodefect coupling) incident light is a source of noise for the SPP, unless the illumination occurs away from the region of interest, increasing the system size and weakening the SPP intensity. Back-side illumination of subwavelength apertures in optically thick metal films eliminates this problem but does not ensure a unique propagation direction for the SPP. We propose a novel back-side slit-illumination method based on drilling a periodic array of indentations at one side of the slit. We demonstrate that the SPP running in the array direction can be suppressed, and the one propagating in the opposite direction enhanced, providing localized unidirectional SPP launching.Comment: 13 pages, 4 figure

Mycobiome of the Bat White Nose Syndrome (WNS) Affected Caves and Mines reveals High Diversity of Fungi and Local Adaptation by the Fungal Pathogen Pseudogymnoascus (Geomyces) destructans

The investigations of the bat White Nose Syndrome (WNS) have yet to provide answers as to how the causative fungus Pseudogymnoascus (Geomyces) destructans (Pd) first appeared in the Northeast and how a single clone has spread rapidly in the US and Canada. We aimed to catalogue Pd and all other fungi (mycobiome) by the culture-dependent (CD) and culture-independent (CI) methods in four Mines and two Caves from the epicenter of WNS zoonotic. Six hundred sixty-five fungal isolates were obtained by CD method including the live recovery of Pd. Seven hundred three nucleotide sequences that met the definition of operational taxonomic units (OTUs) were recovered by CI methods. Most OTUs belonged to unidentified clones deposited in the databases as environmental nucleic acid sequences (ENAS). The core mycobiome of WNS affected sites comprised of 46 species of fungi from 31 genera recovered in culture, and 17 fungal genera and 31 ENAS identified from clone libraries. Fungi such as Arthroderma spp., Geomyces spp., Kernia spp., Mortierella spp., Penicillium spp., and Verticillium spp. were predominant in culture while Ganoderma spp., Geomyces spp., Mortierella spp., Penicillium spp. and Trichosporon spp. were abundant is clone libraries. Alpha diversity analyses from CI data revealed that fungal community structure was highly diverse. However, the true species diversity remains undetermined due to under sampling. The frequent recovery of Pd indicated that the pathogen has adapted to WNS-afflicted habitats. Further, this study supports the hypothesis that Pd is an introduced species. These findings underscore the need for integrated WNS control measures that target both bats and the fungal pathogen.Comment: 59 pages, 7figure

Grating-coupled excitation of multiple surface plasmon-polariton waves

The excitation of multiple surface-plasmon-polariton (SPP) waves of different linear polarization states and phase speeds by a surface-relief grating formed by a metal and a rugate filter, both of finite thickness, was studied theoretically, using rigorous coupled-wave-analysis. The incident plane wave can be either p or s polarized. The excitation of SPP waves is indicated by the presence of those peaks in the plots of absorbance vs. the incidence angle that are independent of the thickness of the rugate filter. The absorbance peaks representing the excitation of s-polarized SPP waves are narrower than those representing p-polarized SPP waves. Two incident plane waves propagating in different directions may excite the same SPP wave. A line source could excite several SPP waves simultaneously

Microbial processes and bacterial populations associated to anaerobic treatment of sulfate-rich wastewater

A pilot-scale (1.2 m3) anaerobic sequencing batch biofilm reactor (ASBBR) containing mineral coal for biomass attachment was fed with sulfate-rich wastewater at increasing sulfate concentrations. Ethanol was used as the main organic source. Tested COD/sulfate ratios were of 1.8 and 1.5 for sulfate loading rates of 0.65–1.90 kgSO42−/cycle (48 h-cycle) or of 1.0 in the trial with 3.0 gSO42− l−1. Sulfate removal efficiencies observed in all trials were as high as 99%. Molecular inventories indicated a shift on the microbial composition and a decrease on species diversity with the increase of sulfate concentration. Beta-proteobacteria species affiliated with Aminomonas spp. and Thermanaerovibrio spp. predominated at 1.0 gSO42− l−1. At higher sulfate concentrations the predominant bacterial group was Delta-proteobacteria mainly Desulfovibrio spp. and Desulfomicrobium spp. at 2.0 gSO42− l−1, whereas Desulfurella spp. and Coprothermobacter spp. predominated at 3.0 gSO42− l−1. These organisms have been commonly associated with sulfate reduction producing acetate, sulfide and sulfur. Methanogenic archaea (Methanosaeta spp.) was found at 1.0 and 2.0 gSO42− l−1. Additionally, a simplified mathematical model was used to infer on metabolic pathways of the biomass involved in sulfate reduction

Tunable THz Surface Plasmon Polariton based on Topological Insulator-Layered Superconductor Hybrid Structure

We theoretically investigate the surface plasmon polariton (SPP) at the interface between 3D strong topological insulator (TI) and layered superconductor-magnetic insulator structure. The tunability of SPP through electronic doping can be enhanced when the magnetic permeability of the layered structure becomes higher. When the interface is gapped by superconductivity or perpendicular magnetism, SPP dispersion is further distorted, accompanied by a shift of group velocity and penetration depth. Such a shift of SPP reaches maximum when the magnitude of Fermi level approaches the gap value, and may lead to observable effects. The tunable SPP at the interface between layered superconductor and magnetism materials in proximity to TI surface may provide new insight in the detection of Majorana Fermions.Comment: 6 pages, 4 figure