Overview: Computer vision and machine learning for microstructural characterization and analysis

The characterization and analysis of microstructure is the foundation of microstructural science, connecting the materials structure to its composition, process history, and properties. Microstructural quantification traditionally involves a human deciding a priori what to measure and then devising a purpose-built method for doing so. However, recent advances in data science, including computer vision (CV) and machine learning (ML) offer new approaches to extracting information from microstructural images. This overview surveys CV approaches to numerically encode the visual information contained in a microstructural image, which then provides input to supervised or unsupervised ML algorithms that find associations and trends in the high-dimensional image representation. CV/ML systems for microstructural characterization and analysis span the taxonomy of image analysis tasks, including image classification, semantic segmentation, object detection, and instance segmentation. These tools enable new approaches to microstructural analysis, including the development of new, rich visual metrics and the discovery of processing-microstructure-property relationships.Comment: submitted to Materials and Metallurgical Transactions

Unsupervised Classification of SAR Images using Hierarchical Agglomeration and EM

We implement an unsupervised classification algorithm for high resolution Synthetic Aperture Radar (SAR) images. The foundation of algorithm is based on Classification Expectation-Maximization (CEM). To get rid of two drawbacks of EM type algorithms, namely the initialization and the model order selection, we combine the CEM algorithm with the hierarchical agglomeration strategy and a model order selection criterion called Integrated Completed Likelihood (ICL). We exploit amplitude statistics in a Finite Mixture Model (FMM), and a Multinomial Logistic (MnL) latent class label model for a mixture density to obtain spatially smooth class segments. We test our algorithm on TerraSAR-X data

Unsupervised Classification of SAR Images using Hierarchical Agglomeration and EM

We implement an unsupervised classification algorithm for high resolution Synthetic Aperture Radar (SAR) images. The foundation of algorithm is based on Classification Expectation-Maximization (CEM). To get rid of two drawbacks of EM type algorithms, namely the initialization and the model order selection, we combine the CEM algorithm with the hierarchical agglomeration strategy and a model order selection criterion called Integrated Completed Likelihood (ICL). We exploit amplitude statistics in a Finite Mixture Model (FMM), and a Multinomial Logistic (MnL) latent class label model for a mixture density to obtain spatially smooth class segments. We test our algorithm on TerraSAR-X data

Lamin A/C sustains PcG protein architecture, maintaining transcriptional repression at target genes

Beyond its role in providing structure to the nuclear envelope, lamin A/C is involved in transcriptional regulation. However, its cross talk with epigenetic factors--and how this cross talk influences physiological processes--is still unexplored. Key epigenetic regulators of development and differentiation are the Polycomb group (PcG) of proteins, organized in the nucleus as microscopically visible foci. Here, we show that lamin A/C is evolutionarily required for correct PcG protein nuclear compartmentalization. Confocal microscopy supported by new algorithms for image analysis reveals that lamin A/C knock-down leads to PcG protein foci disassembly and PcG protein dispersion. This causes detachment from chromatin and defects in PcG protein-mediated higher-order structures, thereby leading to impaired PcG protein repressive functions. Using myogenic differentiation as a model, we found that reduced levels of lamin A/C at the onset of differentiation led to an anticipation of the myogenic program because of an alteration of PcG protein-mediated transcriptional repression. Collectively, our results indicate that lamin A/C can modulate transcription through the regulation of PcG protein epigenetic factors