Beating the reaction limits of biosensor sensitivity with dynamic tracking of single binding events

The clinical need for ultrasensitive molecular analysis has motivated the development of several endpoint-assay technologies capable of single-molecule readout. These endpoint assays are now primarily limited by the affinity and specificity of the molecular-recognition agents for the analyte of interest. In contrast, a kinetic assay with single-molecule readout could distinguish between low-abundance, high-affinity (specific analyte) and high-abundance, low-affinity (nonspecific background) binding by measuring the duration of individual binding events at equilibrium. Here, we describe such a kinetic assay, in which individual binding events are detected and monitored during sample incubation. This method uses plasmonic gold nanorods and interferometric reflectance imaging to detect thousands of individual binding events across a multiplex solid-phase sensor with a large area approaching that of leading bead-based endpoint-assay technologies. A dynamic tracking procedure is used to measure the duration of each event. From this, the total rates of binding and debinding as well as the distribution of binding-event durations are determined. We observe a limit of detection of 19 fM for a proof-of-concept synthetic DNA analyte in a 12-plex assay format.First author draf

Unobtrusive and pervasive video-based eye-gaze tracking

Eye-gaze tracking has long been considered a desktop technology that finds its use inside the traditional office setting, where the operating conditions may be controlled. Nonetheless, recent advancements in mobile technology and a growing interest in capturing natural human behaviour have motivated an emerging interest in tracking eye movements within unconstrained real-life conditions, referred to as pervasive eye-gaze tracking. This critical review focuses on emerging passive and unobtrusive video-based eye-gaze tracking methods in recent literature, with the aim to identify different research avenues that are being followed in response to the challenges of pervasive eye-gaze tracking. Different eye-gaze tracking approaches are discussed in order to bring out their strengths and weaknesses, and to identify any limitations, within the context of pervasive eye-gaze tracking, that have yet to be considered by the computer vision community.peer-reviewe

Infrared face recognition: a comprehensive review of methodologies and databases

Automatic face recognition is an area with immense practical potential which includes a wide range of commercial and law enforcement applications. Hence it is unsurprising that it continues to be one of the most active research areas of computer vision. Even after over three decades of intense research, the state-of-the-art in face recognition continues to improve, benefitting from advances in a range of different research fields such as image processing, pattern recognition, computer graphics, and physiology. Systems based on visible spectrum images, the most researched face recognition modality, have reached a significant level of maturity with some practical success. However, they continue to face challenges in the presence of illumination, pose and expression changes, as well as facial disguises, all of which can significantly decrease recognition accuracy. Amongst various approaches which have been proposed in an attempt to overcome these limitations, the use of infrared (IR) imaging has emerged as a particularly promising research direction. This paper presents a comprehensive and timely review of the literature on this subject. Our key contributions are: (i) a summary of the inherent properties of infrared imaging which makes this modality promising in the context of face recognition, (ii) a systematic review of the most influential approaches, with a focus on emerging common trends as well as key differences between alternative methodologies, (iii) a description of the main databases of infrared facial images available to the researcher, and lastly (iv) a discussion of the most promising avenues for future research.Comment: Pattern Recognition, 2014. arXiv admin note: substantial text overlap with arXiv:1306.160

On Acquisition and Analysis of a Dataset Comprising of Gait, Ear and Semantic data

In outdoor scenarios such as surveillance where there is very little control over the environments, complex computer vision algorithms are often required for analysis. However constrained environments, such as walkways in airports where the surroundings and the path taken by individuals can be controlled, provide an ideal application for such systems. Figure 1.1 depicts an idealised constrained environment. The path taken by the subject is restricted to a narrow path and once inside is in a volume where lighting and other conditions are controlled to facilitate biometric analysis. The ability to control the surroundings and the flow of people greatly simplifes the computer vision task, compared to typical unconstrained environments. Even though biometric datasets with greater than one hundred people are increasingly common, there is still very little known about the inter and intra-subject variation in many biometrics. This information is essential to estimate the recognition capability and limits of automatic recognition systems. In order to accurately estimate the inter- and the intra- class variance, substantially larger datasets are required [40]. Covariates such as facial expression, headwear, footwear type, surface type and carried items are attracting increasing attention; although considering the potentially large impact on an individuals biometrics, large trials need to be conducted to establish how much variance results. This chapter is the first description of the multibiometric data acquired using the University of Southampton's Multi-Biometric Tunnel [26, 37]; a biometric portal using automatic gait, face and ear recognition for identification purposes. The tunnel provides a constrained environment and is ideal for use in high throughput security scenarios and for the collection of large datasets. We describe the current state of data acquisition of face, gait, ear, and semantic data and present early results showing the quality and range of data that has been collected. The main novelties of this dataset in comparison with other multi-biometric datasets are: 1. gait data exists for multiple views and is synchronised, allowing 3D reconstruction and analysis; 2. the face data is a sequence of images allowing for face recognition in video; 3. the ear data is acquired in a relatively unconstrained environment, as a subject walks past; and 4. the semantic data is considerably more extensive than has been available previously. We shall aim to show the advantages of this new data in biometric analysis, though the scope for such analysis is considerably greater than time and space allows for here

The ear as a biometric

It is more than 10 years since the first tentative experiments in ear biometrics were conducted and it has now reached the “adolescence” of its development towards a mature biometric. Here we present a timely retrospective of the ensuing research since those early days. Whilst its detailed structure may not be as complex as the iris, we show that the ear has unique security advantages over other biometrics. It is most unusual, even unique, in that it supports not only visual and forensic recognition, but also acoustic recognition at the same time. This, together with its deep three-dimensional structure and its robust resistance to change with age will make it very difficult to counterfeit thus ensuring that the ear will occupy a special place in situations requiring a high degree of protection