1,053 research outputs found

    Developments in nanoparticles for use in biosensors to assess food safety and quality

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    The following will provide an overview on how advances in nanoparticle technology have contributed towards developing biosensors to screen for safety and quality markers associated with foods. The novel properties of nanoparticles will be described and how such characteristics have been exploited in sensor design will be provided. All the biosensor formats were initially developed for the health care sector to meet the demand for point-of-care diagnostics. As a consequence, research has been directed towards miniaturization thereby reducing the sample volume to nanolitres. However, the needs of the food sector are very different which may ultimately limit commercial application of nanoparticle based nanosensors. © 2014 Elsevier Ltd

    Biosensors: A Fast-Growing Technology for Pathogen Detection in Agriculture and Food Sector

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    Agriculture and food have a greater role to play in order to achieve sustainable development goals. Therefore, there is a need to put an end to the effect of pathogens on food quality and safety. Pathogens have been recognized as one of the major factors causing a reduction in profitable food production. The conventional methods of detecting pathogens are time-consuming and expensive for the farmers in rural areas. In view of this, this chapter reviews the biosensors that have been developed for the detection of biological hazards in food and agricultural sectors. This chapter also lays emphasis on the impact of nanotechnology on building a fast, reliable, more sensitive, accessible, user-friendly and easily adaptable technology for illiterate farmers in the rural communities. On the whole, we have addressed the past and most recent biosensors that could ensure the quick delivery of vision 2030 which aims to end hunger and poverty

    Past, present and future of sensors in food production

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    peer-reviewedMicrobial contamination management is a crucial task in the food industry. Undesirable microbial spoilage in a modern food processing plant poses a risk to consumers’ health, causing severe economic losses to the manufacturers and retailers, contributing to wastage of food and a concern to the world’s food supply. The main goal of the quality management is to reduce the time interval between the filling and the detection of a microorganism before release, from several days, to minutes or, at most, hours. This would allow the food company to stop the production, limiting the damage to just a part of the entire batch, with considerable savings in terms of product value, thereby avoiding the utilization of raw materials, packaging and strongly reducing food waste. Sensor systems offer major advantages over current systems as they are versatile and affordable but need to be integrated in the existing processing systems as a process analytical control (PAT) tool. The desire for good selectivity, low cost, portable and usable at working sites, sufficiently rapid to be used at-line or on-line, and no sample preparation devices are required. The application of biosensors in the food industry still has to compete with the standard analytical techniques in terms of cost, performance and reliability

    Recent Advancements in the Technologies Detecting Food Spoiling Agents

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    To match the current life-style, there is a huge demand and market for the processed food whose manufacturing requires multiple steps. The mounting demand increases the pressure on the producers and the regulatory bodies to provide sensitive, facile, and cost-effective methods to safeguard consumers’ health. In the multistep process of food processing, there are several chances that the food-spoiling microbes or contaminants could enter the supply chain. In this contest, there is a dire necessity to comprehend, implement, and monitor the levels of contaminants by utilizing various available methods, such as single-cell droplet microfluidic system, DNA biosensor, nanobiosensor, smartphone-based biosensor, aptasensor, and DNA microarray-based methods. The current review focuses on the advancements in these methods for the detection of food-borne contaminants and pathogens

    Development and applications of nanobiosensors for sustainable agricultural and food industries : Recent developments, challenges and perspectives

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    The increasing global population and limited natural resources are amongst major challenges in the sustainability of agricultural and food industries, together with the rapid shrinking of land and increasing production cost. Based on the application of nanobiosensors, natural resources can be utilised more efficiently. Particularly, nanobiosensors can be used in a wide range of applications throughout the agri-food route, ranging from detection of soil condition, crop diseases caused by pest/pathogen, management of severe infections, and diagnostic tools for detection of pests during storage and ensures final quality assurance. Here, we review the various recent applications of nanobiosensors in agricultural and food industries. The advantages and limitations are also discussed to provide useful insights to both academic and industrial researchers. Moreover, recent patents have been discussed to provide the latest trends in biosensors for agri-food industry to maintain sustainable development

    Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

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    Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification

    Progress in fluorescence biosensing and food safety towards point-of-detection (PoD) system

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    The detection of pathogens in food substances is of crucial concern for public health and for the safety of the natural environment. Nanomaterials, with their high sensitivity and selectivity have an edge over conventional organic dyes in fluorescent-based detection methods. Advances in microfluidic technology in biosensors have taken place to meet the user criteria of sensitive, inexpensive, user-friendly, and quick detection. In this review, we have summarized the use of fluorescence-based nanomaterials and the latest research approaches towards integrated biosensors, including microsystems containing fluorescence-based detection, various model systems with nano materials, DNA probes, and antibodies. Paper-based lateral-flow test strips and microchips as well as the most-used trapping components are also reviewed, and the possibility of their performance in portable devices evaluated. We also present a current market-available portable system which was developed for food screening and highlight the future direction for the development of fluorescence-based systems for on-site detection and stratification of common foodborne pathogens

    Integration Of Glutaraldehyde Onto Immuno-Membrane For Polyaniline-Based Electrochemical Biosensor

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    Development of membrane for bio-sensing applications for epidemics control has a huge global impact especially for public health. This research explored the electrochemical biosensor with polyaniline (PANI) as a transducer through analyzing the biotin-bovine serum albumin (BSA) interaction as a model system. As membrane appeared as the platform for protein immobilization, selection of suitable membrane is required to create a sensitive and specific biosensor. In measurement of membrane performance, membrane protein binding abilities were quantitatively determined. Among the tested membranes, NC-180 appeared as the most suitable lateral flow membrane as it performed high protein binding ability and short lateral wicking time. To increase the membrane protein binding ability, NC-180 was then subjected to membrane modification using glutaraldehyde (GA) solution. The protein binding of the modified membrane with GA was able to retain up to temperature of 60oC. In the present study, the effect of various integration factors such as the concentration of GA, integration time and number of the integration layer on membrane‟s protein binding ability was investigated. The optimum integration conditions were determined using response surface methodology (RSM)

    Graphene Quantum Dot-Based Electrochemical Immunosensors for Biomedical Applications

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    In the area of biomedicine, research for designing electrochemical sensors has evolved over the past decade, since it is crucial to selectively quantify biomarkers or pathogens in clinical samples for the efficacious diagnosis and/or treatment of various diseases. To fulfil the demand of rapid, specific, economic, and easy detection of such biomolecules in ultralow amounts, numerous nanomaterials have been explored to effectively enhance the sensitivity, selectivity, and reproducibility of immunosensors. Graphene quantum dots (GQDs) have garnered tremendous attention in immunosensor development, owing to their special attributes such as large surface area, excellent biocompatibility, quantum confinement, edge effects, and abundant sites for chemical modification. Besides these distinct features, GQDs acquire peroxidase (POD)-mimicking electro-catalytic activity, and hence, they can replace horseradish peroxidase (HRP)-based systems to conduct facile, quick, and inexpensive label-free immunoassays. The chief motive of this review article is to summarize and focus on the recent advances in GQD-based electrochemical immunosensors for the early and rapid detection of cancer, cardiovascular disorders, and pathogenic diseases. Moreover, the underlying principles of electrochemical immunosensing techniques are also highlighted. These GQD immunosensors are ubiquitous in biomedical diagnosis and conducive for miniaturization, encouraging low-cost disease diagnostics in developing nations using point-of-care testing (POCT) and similar allusive techniques.TU Berlin, Open-Access-Mittel - 201

    Biosensors for European zoonotic agents: a current Portuguese perspective

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    Emerging and recurrent outbreaks caused by zoonotic agents pose a public health risk. They result in morbidity and mortality in humans and significant losses in the livestock and food industries. This highlights the need for rapid surveillance methods. Despite the high reliability of conventional pathogen detection methods, they have high detection limits and are time-consuming and not suitable for on-site analysis. Furthermore, the unpredictable spread of zoonotic infections due to a complex combination of risk factors urges the development of innovative technologies to overcome current limitations in early warning and detection. Biosensing, in particular, is highlighted here, as it offers rapid and cost-effective devices for use at the site of infection while increasing the sensitivity of detection. Portuguese research in biosensors for zoonotic pathogens is the focus of this review. This branch of research produces exciting and innovative devices for the study of the most widespread pathogenic bacteria. The studies presented here relate to the different classes of pathogens whose characteristics and routes of infection are also described. Many advances have been made in recent years, and Portuguese research teams have increased publications in this field. However, biosensing still needs to be extended to other pathogens, including potentially pandemic viruses. In addition, the use of biosensors as part of routine diagnostics in hospitals for humans, in animal infections for veterinary medicine, and food control has not yet been achieved. Therefore, a convergence of Portuguese efforts with global studies on biosensors to control emerging zoonotic diseases is foreseen for the future.Centro de Investigação Desenvolvimento e Inovação da Academia Militar (CINAMIL) from Academia Militar/Instituto Universitário Militar by project SIPA (Sistema Integrado de Proteção Alimentar)info:eu-repo/semantics/publishedVersio
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