Retinal blood vessels extraction using probabilistic modelling

© 2014 Kaba et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.This article has been made available through the Brunel Open Access Publishing Fund.The analysis of retinal blood vessels plays an important role in detecting and treating retinal diseases. In this review, we present an automated method to segment blood vessels of fundus retinal image. The proposed method could be used to support a non-intrusive diagnosis in modern ophthalmology for early detection of retinal diseases, treatment evaluation or clinical study. This study combines the bias correction and an adaptive histogram equalisation to enhance the appearance of the blood vessels. Then the blood vessels are extracted using probabilistic modelling that is optimised by the expectation maximisation algorithm. The method is evaluated on fundus retinal images of STARE and DRIVE datasets. The experimental results are compared with some recently published methods of retinal blood vessels segmentation. The experimental results show that our method achieved the best overall performance and it is comparable to the performance of human experts.The Department of Information Systems, Computing and Mathematics, Brunel University

Retinal vessel segmentation using Gabor Filter and Textons

This paper presents a retinal vessel segmentation method that is inspired by the human visual system and uses a Gabor filter bank. Machine learning is used to optimize the filter parameters for retinal vessel extraction. The filter responses are represented as textons and this allows the corresponding membership functions to be used as the framework for learning vessel and non-vessel classes. Then, vessel texton memberships are used to generate segmentation results. We evaluate our method using the publicly available DRIVE database. It achieves competitive performance (sensitivity=0.7673, specificity=0.9602, accuracy=0.9430) compared to other recently published work. These figures are particularly interesting as our filter bank is quite generic and only includes Gabor responses. Our experimental results also show that the performance, in terms of sensitivity, is superior to other methods

Retinal vessel segmentation using textons

Segmenting vessels from retinal images, like segmentation in many other medical image domains, is a challenging task, as there is no unified way that can be adopted to extract the vessels accurately. However, it is the most critical stage in automatic assessment of various forms of diseases (e.g. Glaucoma, Age-related macular degeneration, diabetic retinopathy and cardiovascular diseases etc.). Our research aims to investigate retinal image segmentation approaches based on textons as they provide a compact description of texture that can be learnt from a training set. This thesis presents a brief review of those diseases and also includes their current situations, future trends and techniques used for their automatic diagnosis in routine clinical applications. The importance of retinal vessel segmentation is particularly emphasized in such applications. An extensive review of previous work on retinal vessel segmentation and salient texture analysis methods is presented. Five automatic retinal vessel segmentation methods are proposed in this thesis. The first method focuses on addressing the problem of removing pathological anomalies (Drusen, exudates) for retinal vessel segmentation, which have been identified by other researchers as a problem and a common source of error. The results show that the modified method shows some improvement compared to a previously published method. The second novel supervised segmentation method employs textons. We propose a new filter bank (MR11) that includes bar detectors for vascular feature extraction and other kernels to detect edges and photometric variations in the image. The k-means clustering algorithm is adopted for texton generation based on the vessel and non-vessel elements which are identified by ground truth. The third improved supervised method is developed based on the second one, in which textons are generated by k-means clustering and texton maps representing vessels are derived by back projecting pixel clusters onto hand labelled ground truth. A further step is implemented to ensure that the best combinations of textons are represented in the map and subsequently used to identify vessels in the test set. The experimental results on two benchmark datasets show that our proposed method performs well compared to other published work and the results of human experts. A further test of our system on an independent set of optical fundus images verified its consistent performance. The statistical analysis on experimental results also reveals that it is possible to train unified textons for retinal vessel segmentation. In the fourth method a novel scheme using Gabor filter bank for vessel feature extraction is proposed. The ii method is inspired by the human visual system. Machine learning is used to optimize the Gabor filter parameters. The experimental results demonstrate that our method significantly enhances the true positive rate while maintaining a level of specificity that is comparable with other approaches. Finally, we proposed a new unsupervised texton based retinal vessel segmentation method using derivative of SIFT and multi-scale Gabor filers. The lack of sufficient quantities of hand labelled ground truth and the high level of variability in ground truth labels amongst experts provides the motivation for this approach. The evaluation results reveal that our unsupervised segmentation method is comparable with the best other supervised methods and other best state of the art methods

Delineation of line patterns in images using B-COSFIRE filters

Delineation of line patterns in images is a basic step required in various applications such as blood vessel detection in medical images, segmentation of rivers or roads in aerial images, detection of cracks in walls or pavements, etc. In this paper we present trainable B-COSFIRE filters, which are a model of some neurons in area V1 of the primary visual cortex, and apply it to the delineation of line patterns in different kinds of images. B-COSFIRE filters are trainable as their selectivity is determined in an automatic configuration process given a prototype pattern of interest. They are configurable to detect any preferred line structure (e.g. segments, corners, cross-overs, etc.), so usable for automatic data representation learning. We carried out experiments on two data sets, namely a line-network data set from INRIA and a data set of retinal fundus images named IOSTAR. The results that we achieved confirm the robustness of the proposed approach and its effectiveness in the delineation of line structures in different kinds of images.Comment: International Work Conference on Bioinspired Intelligence, July 10-13, 201

Detection of retinal blood vessels from ophthalmoscope images using morphological approach

Accurate segmentation of retinal blood vessels is an essential task for diagnosis of various pathological disorders. In this paper, a novel method has been introduced for segmenting retinal blood vessels which involves pre-processing, segmentation and post-processing. The pre-processing stage enhanced the image using contrast limited adaptive histogram equalization and 2D Gabor wavelet. The enhanced image is segmented using geodesic operators and a final segmentation output is obtained by applying a post-processing stage that involves hole filling and removal of isolated pixels. The performance of the proposed method is evaluated on the publicly available Digital retinal images for vessel extraction (DRIVE) and High-resolution fundus (HRF) databases using five different measurements and experimental analysis shows that the proposed method reach an average accuracy of 0.9541 on DRIVE database and 0.9568, 0.9478 and 0.9613 on HRF database with healthy, diabetic retinopathy (DR) and glaucomatous images respectively

Measurement of retinal vessel widths from fundus images based on 2-D modeling

Changes in retinal vessel diameter are an important sign of diseases such as hypertension, arteriosclerosis and diabetes mellitus. Obtaining precise measurements of vascular widths is a critical and demanding process in automated retinal image analysis as the typical vessel is only a few pixels wide. This paper presents an algorithm to measure the vessel diameter to subpixel accuracy. The diameter measurement is based on a two-dimensional difference of Gaussian model, which is optimized to fit a two-dimensional intensity vessel segment. The performance of the method is evaluated against Brinchmann-Hansen's half height, Gregson's rectangular profile and Zhou's Gaussian model. Results from 100 sample profiles show that the presented algorithm is over 30% more precise than the compared techniques and is accurate to a third of a pixel

Retinal Blood Vessel Extraction from Fundus Images Using Enhancement Filtering and Clustering

Screening of vision troubling eye diseases by segmenting fundus images eases the danger of loss of sight of people. Computer assisted analysis can play an important role in the forthcoming health care system universally. Therefore, this paper presents a clustering based method for extraction of retinal vasculature from ophthalmoscope images. The method starts with image enhancement by contrast limited adaptive histogram equalization (CLAHE) from which feature extraction is accomplished using Gabor filter followed by enhancement of extracted features with Hessian based enhancement filters. It then extracts the vessels using K-mean clustering technique. Finally, the method ends with the application of a morphological cleaning operation to get the ultimate vessel segmented image. The performance of the proposed method is evaluated by taking two different publicly available Digital retinal images for vessel extraction (DRIVE) and Child heart and health study in England (CHASE_DB1) databases using nine different performance matrices. It gives average accuracies of 0.952 and 0.951 for DRIVE and CHASE_DB1 databases, respectively.