High-Throughput Screening for Drug Discovery

The book focuses on various aspects and properties of high-throughput screening (HTS), which is of great importance in the development of novel drugs to treat communicable and non-communicable diseases. Chapters in this volume discuss HTS methodologies, resources, and technologies and highlight the significance of HTS in personalized and precision medicine

Full-Duplex OFDM Radar With LTE and 5G NR Waveforms: Challenges, Solutions, and Measurements

This paper studies the processing principles, implementation challenges, and performance of OFDM-based radars, with particular focus on the fourth-generation Long-Term Evolution (LTE) and fifth-generation (5G) New Radio (NR) mobile networks' base stations and their utilization for radar/sensing purposes. First, we address the problem stemming from the unused subcarriers within the LTE and NR transmit signal passbands, and their impact on frequency-domain radar processing. Particularly, we formulate and adopt a computationally efficient interpolation approach to mitigate the effects of such empty subcarriers in the radar processing. We evaluate the target detection and the corresponding range and velocity estimation performance through computer simulations, and show that high-quality target detection as well as high-precision range and velocity estimation can be achieved. Especially 5G NR waveforms, through their impressive channel bandwidths and configurable subcarrier spacing, are shown to provide very good radar/sensing performance. Then, a fundamental implementation challenge of transmitter-receiver (TX-RX) isolation in OFDM radars is addressed, with specific emphasis on shared-antenna cases, where the TX-RX isolation challenges are the largest. It is confirmed that from the OFDM radar processing perspective, limited TX-RX isolation is primarily a concern in detection of static targets while moving targets are inherently more robust to transmitter self-interference. Properly tailored analog/RF and digital self-interference cancellation solutions for OFDM radars are also described and implemented, and shown through RF measurements to be key technical ingredients for practical deployments, particularly from static and slowly moving targets' point of view.Comment: Paper accepted by IEEE Transactions on Microwave Theory and Technique

Transkingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communities

The female reproductive tract (FRT) microbiome plays a vital role in maintaining vaginal health. Viruses are key regulators of other microbial ecosystems, but little is known about how the FRT viruses (virome), particularly bacteriophages that comprise the phageome, impact FRT health and dysbiosis. We hypothesize that bacterial vaginosis (BV) is associated with altered FRT phageome diversity, transkingdom interplay, and bacteriophage discriminate taxa. Here, we conducted a retrospective, longitudinal analysis of vaginal swabs collected from 54 BV-positive and 46 BV-negative South African women. Bacteriome analysis revealed samples clustered into five distinct bacterial community groups (CGs), and further, bacterial alpha diversity was significantly associated with BV. Virome analysis on a subset of baseline samples showed FRT bacteriophages clustering into novel viral state types (VSTs), a viral community clustering system based on virome composition and abundance. Distinct BV bacteriophage signatures included increased alpha diversity along with discriminant Bacillus, Burkholderia, and Escherichia bacteriophages. Bacteriophage-bacteria transkingdom associations were also identified between Bacillus and Burkholderia viruses and BV-associated bacteria, providing key insights for future studies elucidating the transkingdom interactions driving BV-associated microbiome perturbations. In this cohort, bacteriophage-bacterial associations suggest complex interactions, which may play a role in the establishment and maintenance of BV