An investigation into the structure and functional aspects of the cell wall of Desulfovibrio

Satisfactory preparations of cell walls from Desulfovibrio vulgaris cells grown in iron rich (C+Fe) and iron poor (C-Fe) media were obtained by partial detergent solubilisation of the cell envelopes. Polyacrylamide gel electrophoresis (PAGE) has shown the presence in the outer membrane (OM) of three major proteins (OMPs l, 2 and 3) and a relatively homogeneous lipopolysaccharide (LPS) containing no ketodeoxyoctonate (KDO). A protein of OMP 1 PAGE mobility is readily removed by the EDTA treatment of cells showing its loose association with the cell surface. Only a portion of OMP l is removed by digestion or acetate extraction indicating the existence of two proteins, OMP la and lb which comigrate in PAGE. 1251 lactoperoxidase labelling of whole cells shows proteins of OMP 1 and 3 mobility to be exposed on the cell surface. OMPs do not show hydrogenase activity and this enzyme is inactive in C-Fe cells. Numerous minor proteins are present in C+Fe OM but are absent from C-Fe OM indicating ’protection’ of the cell wall by iron to detergent solubilisation. In response to iron limitation D.vulgaris Woolwich shows an increase in the proportion of carbohydrate in the cell wall and in the yield of extractable LPS. HPLC studies have shown changes in the LPS sugars with iron limitation indicating an interaction between Fed I) and the LPS. Studies on the release of LPS from Intact cells by an EDTA washing procedure have shown a selective interaction between Fe2+ and LPS In D.vulgaris. Fe(II) appears to have an important role in the stabilisation in vivo of the OM and this selective interaction may play a part in iron uptake. In vitro OM reconstitutions using extracted material have given further support to this selective interaction. The incorporation of acetate extracted OMPs into reconstituted OM has indicated a possible iron transport function for one or more of these proteins. Calculations of Fe2+ :LPS molar binding ratios based on compositional data and these in vivo and in vitro studies show two forms of iron binding to the surface LPS, which may be applicable to the parameters at work in the natural environment of the bacteria

NASA SBIR abstracts of 1991 phase 1 projects

The objectives of 301 projects placed under contract by the Small Business Innovation Research (SBIR) program of the National Aeronautics and Space Administration (NASA) are described. These projects were selected competitively from among proposals submitted to NASA in response to the 1991 SBIR Program Solicitation. The basic document consists of edited, non-proprietary abstracts of the winning proposals submitted by small businesses. The abstracts are presented under the 15 technical topics within which Phase 1 proposals were solicited. Each project was assigned a sequential identifying number from 001 to 301, in order of its appearance in the body of the report. Appendixes to provide additional information about the SBIR program and permit cross-reference of the 1991 Phase 1 projects by company name, location by state, principal investigator, NASA Field Center responsible for management of each project, and NASA contract number are included

XVIII International Coal Preparation Congress

Changes in economic and market conditions of mineral raw materials in recent years have greatly increased demands on the ef fi ciency of mining production. This is certainly true of the coal industry. World coal consumption is growing faster than other types of fuel and in the past year it exceeded 7.6 billion tons. Coal extraction and processing technology are continuously evolving, becoming more economical and environmentally friendly. “ Clean coal ” technology is becoming increasingly popular. Coal chemistry, production of new materials and pharmacology are now added to the traditional use areas — power industry and metallurgy. The leading role in the development of new areas of coal use belongs to preparation technology and advanced coal processing. Hi-tech modern technology and the increasing interna- tional demand for its effectiveness and ef fi ciency put completely new goals for the University. Our main task is to develop a new generation of workforce capacity and research in line with global trends in the development of science and technology to address critical industry issues. Today Russia, like the rest of the world faces rapid and profound changes affecting all spheres of life. The de fi ning feature of modern era has been a rapid development of high technology, intellectual capital being its main asset and resource. The dynamics of scienti fi c and technological development requires acti- vation of University research activities. The University must be a generator of ideas to meet the needs of the economy and national development. Due to the high intellectual potential, University expert mission becomes more and more called for and is capable of providing professional assessment and building science-based predictions in various fi elds. Coal industry, as well as the whole fuel and energy sector of the global economy is growing fast. Global multinational energy companies are less likely to be under state in fl uence and will soon become the main mechanism for the rapid spread of technologies based on new knowledge. Mineral resources will have an even greater impact on the stability of the economies of many countries. Current progress in the technology of coal-based gas synthesis is not just a change in the traditional energy markets, but the emergence of new products of direct consumption, obtained from coal, such as synthetic fuels, chemicals and agrochemical products. All this requires a revision of the value of coal in the modern world economy

Optimization of dense medium cyclone plant for the beneficiation of low grade iron ore with associated high proportion of near-density material at Sishen Iron Ore Mine

A research report submitted to the Faculty of Engineering and the Built Environment, University of the Witwatersrand, Johannesburg, in partial fulfilment of the requirements for the Degree of Master of Science in Engineering (Metallurgy and Materials Engineering) July 2015The research report is premised on three aspects which are critical in the heavy mineral beneficiation. These aspects are classified as (i) understanding the densimetric profile of the available ore body, (ii) understanding the properties of the heavy medium utilised at the plant to beneficiate the ore, and (iii) the automation and modelling of the processing plant in order to maximise plant efficiency. Ore characterisation is mainly focused on understanding the densimetric profile of the ore body, in order to determine the probability of producing a saleable product as well as predicting the expected yields and quality. This is done to utilise the endowment entrusted upon the operating entity by the government and shareholders to treat the mineral resource to its full potential. Understanding of the beneficiation potential of the ore body will assist the mine planning and processing plant to optimise the product tons and quality. This will ensure the marketing plans are in accordance with the expected product as beneficiation will vary depending on the mining block reserves. The mining blocks have potential to produce varying product grades with different recoveries. Ore characterisation was conducted on the GR80 mining block, low-grade stockpiles (i.e. C-grade ore reserves & Jig discard and dense medium separation (DMS) run-of-mine (ROM) material. The GR80 material was characterised as having low proportion of near-density material and would be easy to beneficiate as well as produce high volumes of high grade product. Furthermore, it was revealed that the 2014 DMS ROM had an increased proportion of low-density material; however this material was also had low proportion of near-density material. The low-grade stockpiles was characterised by high proportion of near density material, which necessitate the beneficiation process to operate at high density in excess of 3.8 t/m3. Maintaining a higher operating density requires more dense medium which leads to viscosity problems and impact performance. The characterisation of the FeSi medium was imperative to understand its behaviour and potential influence on beneficiation of low-grade stockpiles and mining blocks with elevated proportion of near-density material. As the proportion of near-density waste material increases in the run-of-mine (ROM), it is necessary to beneficiate the material at elevated operating medium densities. However, when cyclones are operated at high densities, the negative influence of the medium viscosity becomes more apparent and thus influences the separation efficiency. Heavy medium, ferrosilicon (FeSi) characterisation looked at identifying the effects of viscosity on the FeSi stability and whether there would be a need for a viscosity modifier. Thus, the importance of controlling the stability, viscosity, and density of the medium cannot be under-estimated and can very often override the improvements attainable through better designs of cyclones. Furthermore, the slurry mixture of the heavy medium utilised for the purpose of dense medium separation should be non-detrimental to the effectiveness of separation in the DMS Fine cyclone plant. Medium characterisation showed that removal of ultra-fines leads to unstable media as indicated by faster settling rates. This would result in medium segregation in the beneficiation cyclone thereby leading to unacceptable high density differential which will negatively impact the cut-point shift and cause high yield losses to waste. The overall control of the metallurgical processes at Sishen’s Cyclone Plant is still done on manually and thus operation still varies from person-to-person and/or from shift-to-shift. This result in some of the process data and trends not being available online as well as being captured inaccurately. Furthermore, this negatively affects the traceability and reproducibility of the production metallurgical key performance indicators (KPI’s) as well as process stability and efficiency. It has been demonstrated that real-time online measurements are crucial to maintaining processing plant stability and efficiency thereby ensuring that the final product grade and its value is not eroded. Modelling and automation of the key metallurgical parameters for the cyclone plant circuit was achieved by installation of appropriate instrumentation and interlocking to the programmable logic control (PLC). This allowed for the control of the correct medium sump level, cyclone inlet pressure, medium-to-ore ratio as well as online monitoring of density differential as “proxy” for medium rheological characteristics. The benefit of modelling and simulation allows the virtual investigation and optimisation of the processing plant efficiency as well as analysis of the impact of varying ore characteristics, throughput variations and changing operating parameters. Therefore it is imperative that all cyclone operating modules are operated at the same efficiency which can be achieved by optimized process through proper automation and monitoring, thereby improving the total plant profitability. Keywords: dense medium separation; densimetric profile; dynamic modelling; FeSi rheology; iron-ore beneficiation; process automation; process control

Advances in raw material industries for sustainable development goals

"""Advances in Raw Material Industries for Sustainable Development Goals"" presents the results of joint scientific research conducted in the context of the Russian-German Raw Materials Forum. Today Russia and Germany are exploring various forms of cooperation in the field of mining, geology, mineralogy, mechanical engineering and energy. Russia and Germany are equally interested in expanding cooperation and modernizing the economy in terms of sustainable development. The main theme of this article collection is connected with existing business ventures and ideas from both Russia and Germany. In this book the authors regard complex processes in mining industry from various points of view, including: - modern technologies in prospecting, exploration and development of mineral resources - progressive methods of natural and industrial mineral raw materials processing - energy technologies and digital technologies for sustainable development - cutting-edge technologies and innovations in the oil and gas industry. Working with young researchers, supporting their individual professional development and creating conditions for their mobility and scientific cooperation are essential parts of Russian-German Raw Materials Forum founded in Dresden 13 years ago. This collection represents both willingness of young researchers to be involved in large-scale international projects like Russian-German Raw Material Forum and the results of their long and thorough work in the promising areas of cooperation between Russia and Germany.

Space Bioreactor Science Workshop

The first space bioreactor has been designed for microprocessor control, no gaseous headspace, circulation and resupply of culture medium, and a slow mixing in very low shear regimes. Various ground based bioreactors are being used to test reactor vessel design, on-line sensors, effects of shear, nutrient supply, and waste removal from continuous culture of human cells attached to microcarriers. The small (500 ml) bioreactor is being constructed for flight experiments in the Shuttle middeck to verify systems operation under microgravity conditions and to measure the efficiencies of mass transport, gas transfer, oxygen consumption, and control of low shear stress on cells. Applications of microcarrier cultures, development of the first space bioreactor flight system, shear and mixing effects on cells, process control, and methods to monitor cell metabolism and nutrient requirements are among the topics covered

CESE-2019

This book is a collation of articles published in the Special Issue "CESE-2019: Applications of Membranes" in the journal Sustainability. It contains a wide variety of topics such as the removal of trace organic contaminants using combined direct contact membrane distillation–UV photolysis; evaluating the feasibility of forward osmosis in diluting reverse osmosis concentrate; tailoring the effects of titanium dioxide (TiO2) and polyvinyl alcohol (PVA) in the separation and antifouling performance of thin-film composite polyvinylidene fluoride (PVDF) membrane; enhancing the antibacterial properties of PVDF membrane by surface modification using TiO2 and silver nanoparticles; and reviews on membrane fouling in membrane bioreactor (MBR) systems and recent advances in the prediction of fouling in MBRs. The book is suitable for postgraduate students and researchers working in the field of membrane applications for treating aqueous solutions

A decision support system for ground improvement method selection

Abstract unavailable please refer to PD