12,104 research outputs found
Artificial Rheotaxis
Motility is a basic feature of living microorganisms, and how it works is
often determined by environmental cues. Recent efforts have focused on develop-
ing artificial systems that can mimic microorganisms, and in particular their
self-propulsion. Here, we report on the design and characterization of syn-
thetic self-propelled particles that migrate upstream, known as positive rheo-
taxis. This phenomenon results from a purely physical mechanism involving the
interplay between the polarity of the particles and their alignment by a
viscous torque. We show quantitative agreement between experimental data and a
simple model of an overdamped Brownian pendulum. The model no- tably predicts
the existence of a stagnation point in a diverging flow. We take advantage of
this property to demonstrate that our active particles can sense and
predictably organize in an imposed flow. Our colloidal system represents an
important step towards the realization of biomimetic micro-systems withthe
ability to sense and respond to environmental changesComment: Published in Science Advances [Open access journal of Science
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Mechanism of bidirectional thermotaxis in Escherichia coli.
In bacteria various tactic responses are mediated by the same cellular pathway, but sensing of physical stimuli remains poorly understood. Here, we combine an in-vivo analysis of the pathway activity with a microfluidic taxis assay and mathematical modeling to investigate the thermotactic response of Escherichia coli. We show that in the absence of chemical attractants E. coli exhibits a steady thermophilic response, the magnitude of which decreases at higher temperatures. Adaptation of wild-type cells to high levels of chemoattractants sensed by only one of the major chemoreceptors leads to inversion of the thermotactic response at intermediate temperatures and bidirectional cell accumulation in a thermal gradient. A mathematical model can explain this behavior based on the saturation-dependent kinetics of adaptive receptor methylation. Lastly, we find that the preferred accumulation temperature corresponds to optimal growth in the presence of the chemoattractant serine, pointing to a physiological relevance of the observed thermotactic behavior
Adaptive locomotion of artificial microswimmers
Bacteria can exploit mechanics to display remarkable plasticity in response
to locally changing physical and chemical conditions. Compliant structures play
a striking role in their taxis behavior, specifically for navigation inside
complex and structured environments. Bioinspired mechanisms with rationally
designed architectures capable of large, nonlinear deformation present
opportunities for introducing autonomy into engineered small-scale devices.
This work analyzes the effect of hydrodynamic forces and rheology of local
surroundings on swimming at low Reynolds number, identifies the challenges and
benefits of utilizing elastohydrodynamic coupling in locomotion, and further
develops a suite of machinery for building untethered microrobots with
self-regulated mobility. We demonstrate that coupling the structural and
magnetic properties of artificial microswimmers with the dynamic properties of
the fluid leads to adaptive locomotion in the absence of on-board sensors
Physiological ecology of the ciliated protozoon Loxodes
Loxodes faces special problems in living close to the oxic-anoxic boundary. In tightly-stratified ponds like Priest Pot its optimum environment may be quite narrow and it can be displaced by the slightest turbulence. Loxodes cannot sense an O sub(2) gradient directly but its ability to perceive gravity allows it to make relatively long vertical migrations. It is also sensitive to light and oxygen and it uses these environmental cues to modulate the parameters of its random motility: in the dark, it aggregates at a low O sub(2) tension and in bright light it aggregates in anoxic water. The oxic-anoxic boundary is also a zone where O sub(2) may be a scarce and transient resource, but Loxodes) can switch to nitrate respiration and exploit the pool of nitrate that often exists close to the base of the oxycline
Sperm trajectories form chiral ribbons.
We report the discovery of an entirely new three-dimensional (3D) swimming pattern observed in human and horse sperms. This motion is in the form of 'chiral ribbons', where the planar swing of the sperm head occurs on an osculating plane creating in some cases a helical ribbon and in some others a twisted ribbon. The latter, i.e., the twisted ribbon trajectory, also defines a minimal surface, exhibiting zero mean curvature for all the points on its surface. These chiral ribbon swimming patterns cannot be represented or understood by already known patterns of sperms or other micro-swimmers. The discovery of these unique patterns is enabled by holographic on-chip imaging of >33,700 sperm trajectories at >90-140 frames/sec, which revealed that only ~1.7% of human sperms exhibit chiral ribbons, whereas it increases to ~27.3% for horse sperms. These results might shed more light onto the statistics and biophysics of various micro-swimmers' 3D motion
Identification of the YfgF MASE1 domain as a modulator of bacterial responses to aspartate
Complex 3'-5'-cyclic diguanylic acid (c-di-GMP) responsive regulatory networks that are modulated by the action of multiple diguanylate cyclases (DGC; GGDEF domain proteins) and phosphodiesterases (PDE; EAL domain proteins) have evolved in many bacteria. YfgF proteins possess a membrane-anchoring domain (MASE1), a catalytically inactive GGDEF domain and a catalytically active EAL domain. Here, sustained expression of the Salmonella enterica spp. Enterica ser. Enteritidis YfgF protein is shown to mediate inhibition of the formation of the aspartate chemotactic ring on motility agar under aerobic conditions. This phenomenon was c-di-GMP-independent because it occurred in a Salmonella strain that lacked the ability to synthesize c-di-GMP and also when PDE activity was abolished by site-directed mutagenesis of the EAL domain. YfgF-mediated inhibition of aspartate chemotactic ring formation was impaired in the altered redox environment generated by exogenous p-benzoquinone. This ability of YfgF to inhibit the response to aspartate required a motif, (213)Lys-Lys-Glu(215), in the predicted cytoplasmic loop between trans-membrane regions 5 and 6 of the MASE1 domain. Thus, for the first time the function of a MASE1 domain as a redox-responsive regulator of bacterial responses to aspartate has been shown
Overview of mathematical approaches used to model bacterial chemotaxis I: the single cell
Mathematical modeling of bacterial chemotaxis systems has been influential and insightful in helping to understand experimental observations. We provide here a comprehensive overview of the range of mathematical approaches used for modeling, within a single bacterium, chemotactic processes caused by changes to external gradients in its environment. Specific areas of the bacterial system which have been studied and modeled are discussed in detail, including the modeling of adaptation in response to attractant gradients, the intracellular phosphorylation cascade, membrane receptor clustering, and spatial modeling of intracellular protein signal transduction. The importance of producing robust models that address adaptation, gain, and sensitivity are also discussed. This review highlights that while mathematical modeling has aided in understanding bacterial chemotaxis on the individual cell scale and guiding experimental design, no single model succeeds in robustly describing all of the basic elements of the cell. We conclude by discussing the importance of this and the future of modeling in this area
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