Enhanced perception in volume visualization

Due to the nature of scientic data sets, the generation of convenient visualizations may be a difficult task, but crucial to correctly convey the relevant information of the data. When working with complex volume models, such as the anatomical ones, it is important to provide accurate representations, since a misinterpretation can lead to serious mistakes while diagnosing a disease or planning surgery. In these cases, enhancing the perception of the features of interest usually helps to properly understand the data. Throughout years, researchers have focused on different methods to improve the visualization of volume data sets. For instance, the definition of good transfer functions is a key issue in Volume Visualization, since transfer functions determine how materials are classified. Other approaches are based on simulating realistic illumination models to enhance the spatial perception, or using illustrative effects to provide the level of abstraction needed to correctly interpret the data. This thesis contributes with new approaches to enhance the visual and spatial perception in Volume Visualization. Thanks to the new computing capabilities of modern graphics hardware, the proposed algorithms are capable of modifying the illumination model and simulating illustrative motifs in real time. In order to enhance local details, which are useful to better perceive the shape and the surfaces of the volume, our first contribution is an algorithm that employs a common sharpening operator to modify the lighting applied. As a result, the overall contrast of the visualization is enhanced by brightening the salient features and darkening the deeper regions of the volume model. The enhancement of depth perception in Direct Volume Rendering is also covered in the thesis. To do this, we propose two algorithms to simulate ambient occlusion: a screen-space technique based on using depth information to estimate the amount of light occluded, and a view-independent method that uses the density values of the data set to estimate the occlusion. Additionally, depth perception is also enhanced by adding halos around the structures of interest. Maximum Intensity Projection images provide a good understanding of the high intensity features of the data, but lack any contextual information. In order to enhance the depth perception in such a case, we present a novel technique based on changing how intensity is accumulated. Furthermore, the perception of the spatial arrangement of the displayed structures is also enhanced by adding certain colour cues. The last contribution is a new manipulation tool designed for adding contextual information when cutting the volume. Based on traditional illustrative effects, this method allows the user to directly extrude structures from the cross-section of the cut. As a result, the clipped structures are displayed at different heights, preserving the information needed to correctly perceive them.Debido a la naturaleza de los datos científicos, visualizarlos correctamente puede ser una tarea complicada, pero crucial para interpretarlos de forma adecuada. Cuando se trabaja con modelos de volumen complejos, como es el caso de los modelos anatómicos, es importante generar imágenes precisas, ya que una mala interpretación de las mismas puede producir errores graves en el diagnóstico de enfermedades o en la planificación de operaciones quirúrgicas. En estos casos, mejorar la percepción de las zonas de interés, facilita la comprensión de la información inherente a los datos. Durante décadas, los investigadores se han centrado en el desarrollo de técnicas para mejorar la visualización de datos volumétricos. Por ejemplo, los métodos que permiten definir buenas funciones de transferencia son clave, ya que éstas determinan cómo se clasifican los materiales. Otros ejemplos son las técnicas que simulan modelos de iluminación realista, que permiten percibir mejor la distribución espacial de los elementos del volumen, o bien los que imitan efectos ilustrativos, que proporcionan el nivel de abstracción necesario para interpretar correctamente los datos. El trabajo presentado en esta tesis se centra en mejorar la percepción de los elementos del volumen, ya sea modificando el modelo de iluminación aplicado en la visualización, o simulando efectos ilustrativos. Aprovechando la capacidad de cálculo de los nuevos procesadores gráficos, se describen un conjunto de algoritmos que permiten obtener los resultados en tiempo real. Para mejorar la percepción de detalles locales, proponemos modificar el modelo de iluminación utilizando una conocida herramienta de procesado de imágenes (unsharp masking). Iluminando aquellos detalles que sobresalen de las superficies y oscureciendo las zonas profundas, se mejora el contraste local de la imagen, con lo que se consigue realzar los detalles de superficie. También se presentan diferentes técnicas para mejorar la percepción de la profundidad en Direct Volume Rendering. Concretamente, se propone modificar la iluminación teniendo en cuenta la oclusión ambiente de dos maneras diferentes: la primera utiliza los valores de profundidad en espacio imagen para calcular el factor de oclusión del entorno de cada pixel, mientras que la segunda utiliza los valores de densidad del volumen para aproximar dicha oclusión en cada vóxel. Además de estas dos técnicas, también se propone mejorar la percepción espacial y de la profundidad de ciertas estructuras mediante la generación de halos. La técnica conocida como Maximum Intensity Projection (MIP) permite visualizar los elementos de mayor intensidad del volumen, pero no aporta ningún tipo de información contextual. Para mejorar la percepción de la profundidad, proponemos una nueva técnica basada en cambiar la forma en la que se acumula la intensidad en MIP. También se describe un esquema de color para mejorar la percepción espacial de los elementos visualizados. La última contribución de la tesis es una herramienta de manipulación directa de los datos, que permite preservar la información contextual cuando se realizan cortes en el modelo de volumen. Basada en técnicas ilustrativas tradicionales, esta técnica permite al usuario estirar las estructuras visibles en las secciones de los cortes. Como resultado, las estructuras de interés se visualizan a diferentes alturas sobre la sección, lo que permite al observador percibirlas correctamente

Structural and functional, empirical and modeled connectivity in the cerebral cortex of the rat

[EN] Connectomics data from animal models provide an invaluable opportunity to reveal the complex interplay between structure and function in the mammalian brain. In this work, we investigate the relationship between structural and functional connectivity in the rat brain cortex using a directed anatomical network generated from a carefully curated meta-analysis of published tracing data, along with resting-state functional MRI data obtained from a group of 14 anesthetized Wistar rats. We found a high correspondence between the strength of functional connections, measured as blood oxygen level dependent (BOLD) signal correlations between cortical regions, and the weight of the corresponding anatomical links in the connectome graph (maximum Spearman rank-order correlation rho = 0.48). At the network-level, regions belonging to the same functionally defined community tend to form more mutual weighted connections between each other compared to regions located in different communities. We further found that functional communities in resting-state networks are enriched in densely connected anatomical motifs. Importantly, these higher-order structural subgraphs cannot be explained by lower-order topological properties, suggesting that dense structural patterns support functional associations in the resting brain. Simulations of brain-wide resting-state activity based on neural mass models implemented on the empirical rat anatomical connectome demonstrated high correlation between the simulated and the measured functional connectivity (maximum Pearson correlation rho = 0: 53), further suggesting that the topology of structural connections plays an important role in shaping functional cortical networks.This work was supported in part by the Spanish Ministerio de Economia y Competitividad (MINECO) and FEDER funds under grants BFU2015-64380-C2-1-R (S.C) and BFU2015-64380-C2-2-R (D.M.) and EU Horizon 2020 Program 668863-SyBil-AA grant (S.C.). S.C. acknowledges financial support from the Spanish State Research Agency, through the "Severo Ochoa" Programme for Centres of Excellence in R&D (ref. SEV-2013-0317). A. D.-P., was supported by grant FPU13/01475 from the Spanish Ministerio de Educacion, Cultura y Deporte (MECD). O.S. acknowledges support by the J.S. McDonnell Foundation (#220020387) and the National Institutes of Health (NIH R01 AT009036-01). We are also grateful to Andrea Avena-Koenigsberger and Begona Fernandez for their technical support.Díaz-Parra, A.; Osborn, Z.; Canals Gamoneda, S.; Moratal, D.; Sporns, O. (2017). Structural and functional, empirical and modeled connectivity in the cerebral cortex of the rat. NeuroImage. 159:170-184. https://doi.org/10.1016/j.neuroimage.2017.07.046S17018415

Interactions between genetic variation and cellular environment in skeletal muscle gene expression

From whole organisms to individual cells, responses to environmental conditions are influenced by genetic makeup, where the effect of genetic variation on a trait depends on the environmental context. RNA-sequencing quantifies gene expression as a molecular trait, and is capable of capturing both genetic and environmental effects. In this study, we explore opportunities of using allele-specific expression (ASE) to discover cis-acting genotype-environment interactions (GxE)-genetic effects on gene expression that depend on an environmental condition. Treating 17 common, clinical traits as approximations of the cellular environment of 267 skeletal muscle biopsies, we identify 10 candidate environmental response expression quantitative trait loci (reQTLs) across 6 traits (12 unique gene-environment trait pairs; 10% FDR per trait) including sex, systolic blood pressure, and low-density lipoprotein cholesterol. Although using ASE is in principle a promising approach to detect GxE effects, replication of such signals can be challenging as validation requires harmonization of environmental traits across cohorts and a sufficient sampling of heterozygotes for a transcribed SNP. Comprehensive discovery and replication will require large human transcriptome datasets, or the integration of multiple transcribed SNPs, coupled with standardized clinical phenotyping.Peer reviewe

Annotation and analysis of a large cuticular protein family with the R&R Consensus in Anopheles gambiae

<p>Abstract</p> <p>Background</p> <p>The most abundant family of insect cuticular proteins, the CPR family, is recognized by the R&R Consensus, a domain of about 64 amino acids that binds to chitin and is present throughout arthropods. Several species have now been shown to have more than 100 CPR genes, inviting speculation as to the functional importance of this large number and diversity.</p> <p>Results</p> <p>We have identified 156 genes in <it>Anopheles gambiae </it>that code for putative cuticular proteins in this CPR family, over 1% of the total number of predicted genes in this species. Annotation was verified using several criteria including identification of TATA boxes, INRs, and DPEs plus support from proteomic and gene expression analyses. Two previously recognized CPR classes, RR-1 and RR-2, form separate, well-supported clades with the exception of a small set of genes with long branches whose relationships are poorly resolved. Several of these outliers have clear orthologs in other species. Although both clades are under purifying selection, the RR-1 variant of the R&R Consensus is evolving at twice the rate of the RR-2 variant and is structurally more labile. In contrast, the regions flanking the R&R Consensus have diversified in amino-acid composition to a much greater extent in RR-2 genes compared with RR-1 genes. Many genes are found in compact tandem arrays that may include similar or dissimilar genes but always include just one of the two classes. Tandem arrays of RR-2 genes frequently contain subsets of genes coding for highly similar proteins (sequence clusters). Properties of the proteins indicated that each cluster may serve a distinct function in the cuticle.</p> <p>Conclusion</p> <p>The complete annotation of this large gene family provides insight on the mechanisms of gene family evolution and clues about the need for so many CPR genes. These data also should assist annotation of other <it>Anopheles </it>genes.</p

Simply American: Simplicity in Architectural Arrangement, Construction, and Standards, 1820-1920

The term “simplicity” frequently appeared in American architectural discourse from the nineteenth to early twentieth century. Ironically, this was a historical period associated with the Gilded Age, and an architectural period known for historicism and superfluous ornament. At least, that is how architects and critics from the mid-twentieth century characterized the lack of simplicity in nineteenth century architecture. Their interpretation of simplicity as rejecting nonfunctional ornament and historicist association overlooked the various early modern architectural implications explored throughout nineteenth century architecture. Instead, I explain how and why designers from the nineteenth century desired and approximated simplicity in their work in terms of historical precedents and antecedents, dissemination of designs and ideas through publication, and what I call “quietness” – that a building serves as the background for activities rather than as an object of attention. This dissertation interprets prescriptive literature and also studies construction drawings and extant buildings. There was no single definition of simplicity, even our current assumptions have nuances, but I show projects ranging from Quaker meetinghouses to Chicago skyscrapers with deeper symbolism and significance than a mere plain aesthetic. American architects in this dissertation generally understood simplicity as relations between architecture and people with architecture serving as a background for human activities within and around the building. The chapter on economy considers the planning of a building where arranged rooms with clear functions allowed the building to grow with additions. The chapter on construction considers simplicity through the critique of false construction pretending to follow the ancient construction techniques respecting building materials. The chapter on simple cladding traces the appearance of the building’s exterior from solid walls to a covering representing the character of the building that was independent of the structure. The interiors chapter returns to themes similar to economy by studying the decorations and décor suited to the room’s activities. Finally, simplicity was a high standard unifying purpose and appearance, thus becoming a standard in which designers used to measure their ability to approximate the idea of being simple defended through history, publication, and a sense of modesty