Mammalian Sperm Motility: Observation and Theory

Mammalian spermatozoa motility is a subject of growing importance because of rising human infertility and the possibility of improving animal breeding. We highlight opportunities for fluid and continuum dynamics to provide novel insights concerning the mechanics of these specialized cells, especially during their remarkable journey to the egg. The biological structure of the motile sperm appendage, the flagellum, is described and placed in the context of the mechanics underlying the migration of mammalian sperm through the numerous environments of the female reproductive tract. This process demands certain specific changes to flagellar movement and motility for which further mechanical insight would be valuable, although this requires improved modeling capabilities, particularly to increase our understanding of sperm progression in vivo. We summarize current theoretical studies, highlighting the synergistic combination of imaging and theory in exploring sperm motility, and discuss the challenges for future observational and theoretical studies in understanding the underlying mechanics.\ud Acronyms and Definitions\ud Acrosome: the cap of the sperm head containing enzymes allowing penetration of the zona pellucida via the acrosome reaction\ud Adenosine triphosphate (ATP): the currency unit of chemical energy transfer in living cells\ud Axoneme: a phylogenetically conserved structure within the eukaryotic flagellum consisting of a ring of nine microtubule doublets and a central pair, frequently referred to as 9 + 2\ud Bending moment density: the moment per unit length associated with flagellar bending; it can be divided into a hydrodynamic moment, an elastic moment (from the flagellar bending stiffness), an active moment (generated by dyneins exerting forces between adjacent microtubule doublets), and a passive moment resisting shear\ud Capacitation: the physiological state of a sperm required for fertilization, which is accompanied by the motility patterns associated with hyperactivation, characterized in saline by high-amplitude asymmetric beating\ud Central pair: a pair of microtubules along the length of the axoneme, symmetrically and slightly offset from the axoneme centerline\ud Cumulus oophorus: the outer vestment of the mammalian egg consisting of hundreds of cells radiating out from the egg embedded within a non-Newtonian hyaluronic acid gel\ud Dynein: a molecular motor within the axoneme, attached between adjacent microtubule doublets, that exerts a shearing force to induce axonemal bending\ud Flagellum: a motile cellular appendage that drives the swimming of sperm and other cells; this article focuses on the eukaryotic flagellum\ud Microtubule doublet: a pair of proteinaceous filament structures running the length of the axoneme; dyneins drive their bending, which induces flagellar motion\ud Mid-piece: the region of a sperm flagellum with a mitochondrial sheath, where ATP is generated\ud Oocyte: the egg\ud Outer dense fibers and fibrous sheath: accessory structures reinforcing the mammalian sperm flagellum; the combined axoneme and accessory structures are referred to as 9+9+2\ud Resistive-force theory: an approximation for the local drag of a slender filament element in Stokes flow (or a viscoelastic generalization thereof)\ud Rheotaxis: directed motility in response to the influence of fluid flow\ud Shear: in the context of the flagellum, the relative movement of adjacent microtubule doublets\ud Slender-body theory: an improved approximation for the local drag on a slender filament element in Stokes flow (or a viscoelastic generalization thereof)\ud Zona pellucida: a tough glycoprotein coat between the human egg and the cumulus oophorus, which a sperm must penetrate for successful fertilizatio

Numerical Study of Laryngeal Control of Phonation using Realistic Finite Element Models of a Canine Larynx

While many may take it for granted, the human voice is an incredible feat. An average person can produce a great variety of voices and change voice characteristics agilely even without formal training. Last several decades of research has established that the production of voice is largely a mechanical process: i.e., the sustained vibration of the vocal folds driven by the glottal air flow. Since one only has a single pair of vocal folds, the versatility comes with the ability to change the mechanical status of the vocal folds, including vocal fold length and thickness, tension, and level of adduction, through activation of the laryngeal muscles. However, the relationship between laryngeal muscle activity and the characteristics of voice is not well understood due to limitations in experimental observation and simplifications in modelling and simulations. The science is still far behind the art. The current research aims to investigate first the relationship between laryngeal muscle activation and the posture of the vocal folds and second the relationship between voice source characteristics and vocal fold mechanical status using more comprehensive numerical models and simulations, thus improving the understanding of the roles of each laryngeal muscle in voice control. To do so, (1) the mechanics involved in vocal fold posturing and vibration, especially muscle contraction; (2) the realistic anatomical structure of the larynx must be considered properly. To achieve this goal, a numerical model of the larynx as realistic as possible was built. The geometry of the laryngeal components was reconstructed from high resolution MRI (Magnetic Resonance Imaging) data of an excised canine larynx, which makes more accurate the representation of the muscles and their sub-compartments, cartilages, and other important anatomical features of the larynx. A previously proposed muscle activation model was implemented in a 3D finite element package and applied to the larynx model to simulate the action of laryngeal muscles. After validation of the numerical model against experimental data, extensive parametric studies involving different combination of muscle activations were conducted to investigate how the voice source is controlled with laryngeal muscles. In the course of this study, some work was done to couple the same finite element tool with a Genetic Algorithm program to inversely determine model parameters in biomechanical models. The method was applied in a collaborated study on shape changes of a fish fin during swimming. This study is presented as a separate chapter at the end of this thesis. The method has potential application in determining parameters in vocal fold models and optimizing clinical vocal fold procedures. This thesis is essentially an assembly of the papers published by the author during the doctoral study, with the addition of an introductory chapter. Chapter 1 reviews the overall principles of voice production, the biomechanical basis of voice control, and past studies on voice control with a focus on the fundamental frequency. Chapter 2 describes the major numerical methods employed in this research with an emphasis on the finite element method. The muscle activation model is also described in this chapter. Chapter 3 describes the building of the larynx model from MRI data and its partial validation. Chapter 4 presents the application of the larynx model to posturing studies, including parametric activation of muscle groups and specific topics related to vocal fold posturing. Chapter 5 describes the change of vocal fold vibration dynamics under the influence of the interaction of the cricothyroid muscle and the thyroarytenoid muscle. The Flow-structure interaction simulations was realized by coupling the larynx model to a simple Bernoulli flow model and a two-stage simulation technique. Chapter 6 concludes the current thesis study. Suggestions for future studies are proposed. Chapter 7 is an independent study that is not related to voice control. It describes a numerical framework that inversely determines and validates model parameters of biomechanical models. The application of the proposed framework to a finite element model of a fish fin is presented

Multi-user virtual environments for physical education and sport training

For effective learning and training, virtual environments may provide lifelike opportunities, and researchers are actively investigating their potential for educational purposes. Minimal research attention has been paid to the integration of multi-user virtual environments (MUVE) technology for teaching and practicing real sports. In this chapter, the authors reviewed the justifications, possibilities, challenges, and future directions of using MUVE systems. The authors addressed issues such as informal learning, design, engagement, collaboration, learning style, learning evaluation, motivation, and gender, followed by the identification of required elements for successful implementations. In the second part, the authors talked about exergames, the necessity of evaluation, and examples on exploring the behavior of players during playing. Finally, insights on the application of sports exergames in teaching, practicing, and encouraging real sports were discussed