Optogenetic Brain Interfaces

The brain is a large network of interconnected neurons where each cell functions as a nonlinear processing element. Unraveling the mysteries of information processing in the complex networks of the brain requires versatile neurostimulation and imaging techniques. Optogenetics is a new stimulation method which allows the activity of neurons to be modulated by light. For this purpose, the cell-types of interest are genetically targeted to produce light-sensitive proteins. Once these proteins are expressed, neural activity can be controlled by exposing the cells to light of appropriate wavelengths. Optogenetics provides a unique combination of features, including multimodal control over neural function and genetic targeting of specific cell-types. Together, these versatile features combine to a powerful experimental approach, suitable for the study of the circuitry of psychiatric and neurological disorders. The advent of optogenetics was followed by extensive research aimed to produce new lines of light-sensitive proteins and to develop new technologies: for example, to control the distribution of light inside the brain tissue or to combine optogenetics with other modalities including electrophysiology, electrocorticography, nonlinear microscopy, and functional magnetic resonance imaging. In this paper, the authors review some of the recent advances in the field of optogenetics and related technologies and provide their vision for the future of the field.United States. Defense Advanced Research Projects Agency (Space and Naval Warfare Systems Center, Pacific Grant/Contract No. N66001-12-C-4025)University of Wisconsin--Madison (Research growth initiative; grant 101X254)University of Wisconsin--Madison (Research growth initiative; grant 101X172)University of Wisconsin--Madison (Research growth initiative; grant 101X213)National Science Foundation (U.S.) (MRSEC DMR-0819762)National Science Foundation (U.S.) (NSF CAREER CBET-1253890)National Institutes of Health (U.S.) (NIH/NIBIB R00 Award (4R00EB008738)National Institutes of Health (U.S.) (NIH Director’s New Innovator award (1-DP2-OD002989))Okawa Foundation (Research Grant Award)National Institutes of Health (U.S.) (NIH Director’s New Innovator Award (1DP2OD007265))National Science Foundation (U.S.) (NSF CAREER Award (1056008)Alfred P. Sloan Foundation (Fellowship)Human Frontier Science Program (Strasbourg, France) (Grant No. 1351/12)Israeli Centers of Research Excellence (I-CORE grant, program 51/11)MINERVA Foundation (Germany

A quasi-real-time inertialess microwave holographic imaging system

This thesis records the theoretical analysis and hardware development of a laboratory microwave imaging system which uses holographic principles. The application of an aperture synthesis technique and the electronic commutation of all antennae has resulted in a compact and economic assembly - which requires no moving parts and which, consequently, has a high field mapping speed potential. The relationship of this microwave holographic system to other established techniques is examined theoretically and the performance of the imaging system is demonstrated using conventional optically- and numerically-based reconstruction of the measured holograms. The high mapping speed potential of this system has allowed the exploitation of an imaging mode not usually associated with microwave holography. In particular, a certain antenna array specification leads to a versatile imaging system which corresponds closely in the laboratory scale to the widely used synthetic aperture radar principle. It is envisaged that the microwave holographic implementation of this latter principle be used as laboratory instrumentation in the elucidation of the interaction of hydrodynamic and electromagnetic waves. Some simple demonstrations of this application have been presented, and the concluding chapter also describes a suitable hardware specification. This thesis has also emphasised the hardware details of the imaging system since the development of the microwave and other electronic components represented a substantial part of this research and because the potential applications of the imaging principle have been found to be intimately linked to the tolerances of the various microwave components. Bibliography: pages 122-132

COMPRESSIVE IMAGING AND DUAL MOIRE´ LASER INTERFEROMETER AS METROLOGY TOOLS

Metrology is the science of measurement and deals with measuring different physical aspects of objects. In this research the focus has been on two basic problems that metrologists encounter. The first problem is the trade-off between the range of measurement and the corresponding resolution; measurement of physical parameters of a large object or scene accompanies by losing detailed information about small regions of the object. Indeed, instruments and techniques that perform coarse measurements are different from those that make fine measurements. This problem persists in the field of surface metrology, which deals with accurate measurement and detailed analysis of surfaces. For example, laser interferometry is used for fine measurement (in nanometer scale) while to measure the form of in object, which lies in the field of coarse measurement, a different technique like moire technique is used. We introduced a new technique to combine measurement from instruments with better resolution and smaller measurement range with those with coarser resolution and larger measurement range. We first measure the form of the object with coarse measurement techniques and then make some fine measurement for features in regions of interest. The second problem is the measurement conditions that lead to difficulties in measurement. These conditions include low light condition, large range of intensity variation, hyperspectral measurement, etc. Under low light condition there is not enough light for detector to detect light from object, which results in poor measurements. Large range of intensity variation results in a measurement with some saturated regions on the camera as well as some dark regions. We use compressive sampling based imaging systems to address these problems. Single pixel compressive imaging uses a single detector instead of array of detectors and reconstructs a complete image after several measurements. In this research we examined compressive imaging for different applications including low light imaging, high dynamic range imaging and hyperspectral imaging