Microparticle image processing and field profile optimisation for automated Lab-On-Chip magnetophoretic analytical systems

The work described in this thesis, concerns developments to analytical microfluidic Lab-On-Chip platform originally developed by Prof Pamme's research group at the University of Hull. This work aims to move away from traditional laboratory analysis system towards a more effective system design which is fully automated and therefore potentially deployable in applications such as point of care medical diagnosis. The microfluidic chip platform comprises an external permanent magnet and chip with multiple parallel reagent streams through which magnetic micro-particles pass in sequence. These streams may include particles, analyte, fluorescent labels and wash solutions; together they facilitate an on-chip multi-step analytical procedure. Analyte concentration is measured via florescent intensity of the exiting micro-particles. This has previously been experimentally proven for more than one analytical procedure. The work described here has addressed a couple of issues which needed improvement, specifically optimizing the magnetic field and automating the measurement process. These topics are related by the fact that an optimal field will reduce anomalies such as aggregated particles which may degrade automated measurements.For this system, the optimal magnetic field is homogeneous gradient of sufficient strength to pull the particles across the width of the device during fluid transit of its length. To optimise the magnetic field, COMSOL (a Multiphysics simulation program) was used to evaluate a number of multiple magnet configurations and demonstrate an improved field profile. The simulation approach was validated against experimental data for the original single-magnet design.To analyse the results automatically, a software tool has been developed using C++ which takes image files generated during an experiment and outputs a calibration curve or specific measurement result. The process involves detection of the particles (using image segmentation) and object tracking. The intensity measurement follows the same procedure as the original manual approach, facilitating comparison, but also includes analysis of particle motion behaviour to allow automatic rejection of data from anomalous particles (e.g. stuck particles). For image segmentation a novel texture based technique called Temporal- Adaptive Median Binary Pattern (T-AMBP) combining with Three Frame Difference method to model the background for representing the foreground was proposed. This proposed approached is based on previously developed Adaptive Median Binary Pattern (AMBP) and Gaussian Mixture Model (GMM) approach for image segmentation. The proposed method successfully detects micro-particles even when they have very low fluorescent intensity, while most of the previous approaches failed and is more robust to noise and artefacts. For tracking the micro-particles, we proposed a novel algorithm called "Hybrid Meanshift", which combines Meanshift, Histogram of oriented gradients (HOG) matching and optical flow techniques. Kalman filter was also combined with it to make the tracking robust.The processing of an experimental data set for generating a calibration curve, getting effectively the same results in less than 5 minutes was demonstrated, without needing experimental experience, compared with at least 2 hours work by an experienced experimenter using the manual approach

Object Detection and Tracking in Wide Area Surveillance Using Thermal Imagery

The main objective behind this thesis is to examine how existing vision-based detection and tracking algorithms perform in thermal imagery-based video surveillance. While color-based surveillance has been extensively studied, these techniques can not be used during low illumination, at night, or with lighting changes and shadows which limits their applicability. The main contributions in this thesis are (1) the creation of a new color-thermal dataset, (2) a detailed performance comparison of different color-based detection and tracking algorithms on thermal data and (3) the proposal of an adaptive neural network for false detection rejection. Since there are not many publicly available datasets for thermal-video surveillance, a new UNLV Thermal Color Pedestrian Dataset was collected to evaluate the performance of popular color-based detection and tracking in thermal images. The dataset provides an overhead view of humans walking through a courtyard and is appropriate for aerial surveillance scenarios such as unmanned aerial systems (UAS). Three popular detection schemes are studied for thermal pedestrian detection: 1) Haar-like features, 2) local binary pattern (LBP) and 3) background subtraction motion detection. A i) Kalman filter predictor and ii) optical flow are used for tracking. Results show that combining Haar and LBP detections with a 50% overlap rule and tracking using Kalman filters can improve the true positive rate (TPR) of detection by 20%. However, motion-based methods are better at rejecting false positive in non-moving camera scenarios. The Kalman filter with LBP detection is the most efficient tracker but optical flow better rejects false noise detections. This thesis also presents a technique for learning and characterizing pedestrian detections with heat maps and an object-centric motion compensation method for UAS. Finally, an adaptive method to reject false detections using error back propagation using a neural network. The adaptive rejection scheme is able to successfully learn to identify static false detections for improved detection performance

Object Tracking

Object tracking consists in estimation of trajectory of moving objects in the sequence of images. Automation of the computer object tracking is a difficult task. Dynamics of multiple parameters changes representing features and motion of the objects, and temporary partial or full occlusion of the tracked objects have to be considered. This monograph presents the development of object tracking algorithms, methods and systems. Both, state of the art of object tracking methods and also the new trends in research are described in this book. Fourteen chapters are split into two sections. Section 1 presents new theoretical ideas whereas Section 2 presents real-life applications. Despite the variety of topics contained in this monograph it constitutes a consisted knowledge in the field of computer object tracking. The intention of editor was to follow up the very quick progress in the developing of methods as well as extension of the application

Adaptive visual sampling

PhDVarious visual tasks may be analysed in the context of sampling from the visual field. In visual psychophysics, human visual sampling strategies have often been shown at a high-level to be driven by various information and resource related factors such as the limited capacity of the human cognitive system, the quality of information gathered, its relevance in context and the associated efficiency of recovering it. At a lower-level, we interpret many computer vision tasks to be rooted in similar notions of contextually-relevant, dynamic sampling strategies which are geared towards the filtering of pixel samples to perform reliable object association. In the context of object tracking, the reliability of such endeavours is fundamentally rooted in the continuing relevance of object models used for such filtering, a requirement complicated by realworld conditions such as dynamic lighting that inconveniently and frequently cause their rapid obsolescence. In the context of recognition, performance can be hindered by the lack of learned context-dependent strategies that satisfactorily filter out samples that are irrelevant or blunt the potency of models used for discrimination. In this thesis we interpret the problems of visual tracking and recognition in terms of dynamic spatial and featural sampling strategies and, in this vein, present three frameworks that build on previous methods to provide a more flexible and effective approach. Firstly, we propose an adaptive spatial sampling strategy framework to maintain statistical object models for real-time robust tracking under changing lighting conditions. We employ colour features in experiments to demonstrate its effectiveness. The framework consists of five parts: (a) Gaussian mixture models for semi-parametric modelling of the colour distributions of multicolour objects; (b) a constructive algorithm that uses cross-validation for automatically determining the number of components for a Gaussian mixture given a sample set of object colours; (c) a sampling strategy for performing fast tracking using colour models; (d) a Bayesian formulation enabling models of object and the environment to be employed together in filtering samples by discrimination; and (e) a selectively-adaptive mechanism to enable colour models to cope with changing conditions and permit more robust tracking. Secondly, we extend the concept to an adaptive spatial and featural sampling strategy to deal with very difficult conditions such as small target objects in cluttered environments undergoing severe lighting fluctuations and extreme occlusions. This builds on previous work on dynamic feature selection during tracking by reducing redundancy in features selected at each stage as well as more naturally balancing short-term and long-term evidence, the latter to facilitate model rigidity under sharp, temporary changes such as occlusion whilst permitting model flexibility under slower, long-term changes such as varying lighting conditions. This framework consists of two parts: (a) Attribute-based Feature Ranking (AFR) which combines two attribute measures; discriminability and independence to other features; and (b) Multiple Selectively-adaptive Feature Models (MSFM) which involves maintaining a dynamic feature reference of target object appearance. We call this framework Adaptive Multi-feature Association (AMA). Finally, we present an adaptive spatial and featural sampling strategy that extends established Local Binary Pattern (LBP) methods and overcomes many severe limitations of the traditional approach such as limited spatial support, restricted sample sets and ad hoc joint and disjoint statistical distributions that may fail to capture important structure. Our framework enables more compact, descriptive LBP type models to be constructed which may be employed in conjunction with many existing LBP techniques to improve their performance without modification. The framework consists of two parts: (a) a new LBP-type model known as Multiscale Selected Local Binary Features (MSLBF); and (b) a novel binary feature selection algorithm called Binary Histogram Intersection Minimisation (BHIM) which is shown to be more powerful than established methods used for binary feature selection such as Conditional Mutual Information Maximisation (CMIM) and AdaBoost

Detecting and tracking people in real-time

The problem of detecting and tracking people in images and video has been the subject of a great deal of research, but remains a challenging task. Being able to detect and track people would have an impact in a number of fields, such as driverless vehicles, automated surveillance, and human-computer interaction. The difficulties that must be overcome include coping with variations in appearance between different people, changes in lighting, and the ability to detect people across multiple scales. As well as having high accuracy, it is desirable for a technique to evaluate an image with low latency between receiving the image and producing a result. This thesis explores methods for detecting and tracking people in images and video. Techniques are implemented on a desktop computer, with an emphasis on low latency. The problem of detection is examined first. The well established integral channel features detector is introduced and reimplemented, and various novelties are implemented in regards to the features used by the detector. Results are given to quantify the accuracy and the speed of the developed detectors on the INRIA person dataset. The method is further extended by examining the prospect of using multiple classifiers in conjunction. It is shown that using a classifier with a version of the same classifier reflected in the vertical axis can improve performance. A novel method for clustering images of people to find modes of appearance is also presented. This involves using boosting classifiers to map a set of images to vectors, to which K-means clustering is applied. Boosting classifiers are then trained on these clustered datasets to create sets of multiple classifiers, and it is demonstrated that these sets of classifiers can be evaluated on images with only a small increase in the running time over single classifiers. The problem of single target tracking is addressed using the mean shift algorithm. Mean shift tracking works by finding the best colour match for a target from frame to frame. A novel form of mean shift tracking through scale is developed, and the problem of multiple target tracking is addressed by using boosting classifiers in conjunction with Kalman filters. Tests are carried out on the CAVIAR dataset, which gives representative examples of surveillance scenarios, to show the performance of the proposed approaches.Open Acces

Spatial and temporal background modelling of non-stationary visual scenes

PhDThe prevalence of electronic imaging systems in everyday life has become increasingly apparent in recent years. Applications are to be found in medical scanning, automated manufacture, and perhaps most significantly, surveillance. Metropolitan areas, shopping malls, and road traffic management all employ and benefit from an unprecedented quantity of video cameras for monitoring purposes. But the high cost and limited effectiveness of employing humans as the final link in the monitoring chain has driven scientists to seek solutions based on machine vision techniques. Whilst the field of machine vision has enjoyed consistent rapid development in the last 20 years, some of the most fundamental issues still remain to be solved in a satisfactory manner. Central to a great many vision applications is the concept of segmentation, and in particular, most practical systems perform background subtraction as one of the first stages of video processing. This involves separation of ‘interesting foreground’ from the less informative but persistent background. But the definition of what is ‘interesting’ is somewhat subjective, and liable to be application specific. Furthermore, the background may be interpreted as including the visual appearance of normal activity of any agents present in the scene, human or otherwise. Thus a background model might be called upon to absorb lighting changes, moving trees and foliage, or normal traffic flow and pedestrian activity, in order to effect what might be termed in ‘biologically-inspired’ vision as pre-attentive selection. This challenge is one of the Holy Grails of the computer vision field, and consequently the subject has received considerable attention. This thesis sets out to address some of the limitations of contemporary methods of background segmentation by investigating methods of inducing local mutual support amongst pixels in three starkly contrasting paradigms: (1) locality in the spatial domain, (2) locality in the shortterm time domain, and (3) locality in the domain of cyclic repetition frequency. Conventional per pixel models, such as those based on Gaussian Mixture Models, offer no spatial support between adjacent pixels at all. At the other extreme, eigenspace models impose a structure in which every image pixel bears the same relation to every other pixel. But Markov Random Fields permit definition of arbitrary local cliques by construction of a suitable graph, and 3 are used here to facilitate a novel structure capable of exploiting probabilistic local cooccurrence of adjacent Local Binary Patterns. The result is a method exhibiting strong sensitivity to multiple learned local pattern hypotheses, whilst relying solely on monochrome image data. Many background models enforce temporal consistency constraints on a pixel in attempt to confirm background membership before being accepted as part of the model, and typically some control over this process is exercised by a learning rate parameter. But in busy scenes, a true background pixel may be visible for a relatively small fraction of the time and in a temporally fragmented fashion, thus hindering such background acquisition. However, support in terms of temporal locality may still be achieved by using Combinatorial Optimization to derive shortterm background estimates which induce a similar consistency, but are considerably more robust to disturbance. A novel technique is presented here in which the short-term estimates act as ‘pre-filtered’ data from which a far more compact eigen-background may be constructed. Many scenes entail elements exhibiting repetitive periodic behaviour. Some road junctions employing traffic signals are among these, yet little is to be found amongst the literature regarding the explicit modelling of such periodic processes in a scene. Previous work focussing on gait recognition has demonstrated approaches based on recurrence of self-similarity by which local periodicity may be identified. The present work harnesses and extends this method in order to characterize scenes displaying multiple distinct periodicities by building a spatio-temporal model. The model may then be used to highlight abnormality in scene activity. Furthermore, a Phase Locked Loop technique with a novel phase detector is detailed, enabling such a model to maintain correct synchronization with scene activity in spite of noise and drift of periodicity. This thesis contends that these three approaches are all manifestations of the same broad underlying concept: local support in each of the space, time and frequency domains, and furthermore, that the support can be harnessed practically, as will be demonstrated experimentally

Biometric Systems

Because of the accelerating progress in biometrics research and the latest nation-state threats to security, this book's publication is not only timely but also much needed. This volume contains seventeen peer-reviewed chapters reporting the state of the art in biometrics research: security issues, signature verification, fingerprint identification, wrist vascular biometrics, ear detection, face detection and identification (including a new survey of face recognition), person re-identification, electrocardiogram (ECT) recognition, and several multi-modal systems. This book will be a valuable resource for graduate students, engineers, and researchers interested in understanding and investigating this important field of study

Video foreground extraction for mobile camera platforms

Foreground object detection is a fundamental task in computer vision with many applications in areas such as object tracking, event identification, and behavior analysis. Most conventional foreground object detection methods work only in a stable illumination environments using fixed cameras. In real-world applications, however, it is often the case that the algorithm needs to operate under the following challenging conditions: drastic lighting changes, object shape complexity, moving cameras, low frame capture rates, and low resolution images. This thesis presents four novel approaches for foreground object detection on real-world datasets using cameras deployed on moving vehicles.The first problem addresses passenger detection and tracking tasks for public transport buses investigating the problem of changing illumination conditions and low frame capture rates. Our approach integrates a stable SIFT (Scale Invariant Feature Transform) background seat modelling method with a human shape model into a weighted Bayesian framework to detect passengers. To deal with the problem of tracking multiple targets, we employ the Reversible Jump Monte Carlo Markov Chain tracking algorithm. Using the SVM classifier, the appearance transformation models capture changes in the appearance of the foreground objects across two consecutives frames under low frame rate conditions. In the second problem, we present a system for pedestrian detection involving scenes captured by a mobile bus surveillance system. It integrates scene localization, foreground-background separation, and pedestrian detection modules into a unified detection framework. The scene localization module performs a two stage clustering of the video data.In the first stage, SIFT Homography is applied to cluster frames in terms of their structural similarity, and the second stage further clusters these aligned frames according to consistency in illumination. This produces clusters of images that are differential in viewpoint and lighting. A kernel density estimation (KDE) technique for colour and gradient is then used to construct background models for each image cluster, which is further used to detect candidate foreground pixels. Finally, using a hierarchical template matching approach, pedestrians can be detected.In addition to the second problem, we present three direct pedestrian detection methods that extend the HOG (Histogram of Oriented Gradient) techniques (Dalal and Triggs, 2005) and provide a comparative evaluation of these approaches. The three approaches include: a) a new histogram feature, that is formed by the weighted sum of both the gradient magnitude and the filter responses from a set of elongated Gaussian filters (Leung and Malik, 2001) corresponding to the quantised orientation, which we refer to as the Histogram of Oriented Gradient Banks (HOGB) approach; b) the codebook based HOG feature with branch-and-bound (efficient subwindow search) algorithm (Lampert et al., 2008) and; c) the codebook based HOGB approach.In the third problem, a unified framework that combines 3D and 2D background modelling is proposed to detect scene changes using a camera mounted on a moving vehicle. The 3D scene is first reconstructed from a set of videos taken at different times. The 3D background modelling identifies inconsistent scene structures as foreground objects. For the 2D approach, foreground objects are detected using the spatio-temporal MRF algorithm. Finally, the 3D and 2D results are combined using morphological operations.The significance of these research is that it provides basic frameworks for automatic large-scale mobile surveillance applications and facilitates many higher-level applications such as object tracking and behaviour analysis

Enabling Artificial Intelligence Analytics on The Edge

This thesis introduces a novel distributed model for handling in real-time, edge-based video analytics. The novelty of the model relies on decoupling and distributing the services into several decomposed functions, creating virtual function chains (V F C model). The model considers both computational and communication constraints. Theoretical, simulation and experimental results have shown that the V F C model can enable the support of heavy-load services to an edge environment while improving the footprint of the service compared to state-of-the art frameworks. In detail, results on the V F C model have shown that it can reduce the total edge cost, compared with a monolithic and a simple frame distribution models. For experimenting on a real-case scenario, a testbed edge environment has been developed, where the aforementioned models, as well as a general distribution framework (Apache Spark ©), have been deployed. A cloud service has also been considered. Experiments have shown that V F C can outperform all alternative approaches, by reducing operational cost and improving the QoS. Finally, a migration model, a caching model and a QoS monitoring service based on Long-Term-Short-Term models are introduced