Dynamic multifactor hubs interact transiently with sites of active transcription in Drosophila embryos.

The regulation of transcription requires the coordination of numerous activities on DNA, yet how transcription factors mediate these activities remains poorly understood. Here, we use lattice light-sheet microscopy to integrate single-molecule and high-speed 4D imaging in developing Drosophila embryos to study the nuclear organization and interactions of the key transcription factors Zelda and Bicoid. In contrast to previous studies suggesting stable, cooperative binding, we show that both factors interact with DNA with surprisingly high off-rates. We find that both factors form dynamic subnuclear hubs, and that Bicoid binding is enriched within Zelda hubs. Remarkably, these hubs are both short lived and interact only transiently with sites of active Bicoid-dependent transcription. Based on our observations, we hypothesize that, beyond simply forming bridges between DNA and the transcription machinery, transcription factors can organize other proteins into hubs that transiently drive multiple activities at their gene targets.Editorial noteThis article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter)

Streaming Video over HTTP with Consistent Quality

In conventional HTTP-based adaptive streaming (HAS), a video source is encoded at multiple levels of constant bitrate representations, and a client makes its representation selections according to the measured network bandwidth. While greatly simplifying adaptation to the varying network conditions, this strategy is not the best for optimizing the video quality experienced by end users. Quality fluctuation can be reduced if the natural variability of video content is taken into consideration. In this work, we study the design of a client rate adaptation algorithm to yield consistent video quality. We assume that clients have visibility into incoming video within a finite horizon. We also take advantage of the client-side video buffer, by using it as a breathing room for not only network bandwidth variability, but also video bitrate variability. The challenge, however, lies in how to balance these two variabilities to yield consistent video quality without risking a buffer underrun. We propose an optimization solution that uses an online algorithm to adapt the video bitrate step-by-step, while applying dynamic programming at each step. We incorporate our solution into PANDA -- a practical rate adaptation algorithm designed for HAS deployment at scale.Comment: Refined version submitted to ACM Multimedia Systems Conference (MMSys), 201

Motion estimation and CABAC VLSI co-processors for real-time high-quality H.264/AVC video coding

Real-time and high-quality video coding is gaining a wide interest in the research and industrial community for different applications. H.264/AVC, a recent standard for high performance video coding, can be successfully exploited in several scenarios including digital video broadcasting, high-definition TV and DVD-based systems, which require to sustain up to tens of Mbits/s. To that purpose this paper proposes optimized architectures for H.264/AVC most critical tasks, Motion estimation and context adaptive binary arithmetic coding. Post synthesis results on sub-micron CMOS standard-cells technologies show that the proposed architectures can actually process in real-time 720 × 480 video sequences at 30 frames/s and grant more than 50 Mbits/s. The achieved circuit complexity and power consumption budgets are suitable for their integration in complex VLSI multimedia systems based either on AHB bus centric on-chip communication system or on novel Network-on-Chip (NoC) infrastructures for MPSoC (Multi-Processor System on Chip

QoE-Based Low-Delay Live Streaming Using Throughput Predictions

Recently, HTTP-based adaptive streaming has become the de facto standard for video streaming over the Internet. It allows clients to dynamically adapt media characteristics to network conditions in order to ensure a high quality of experience, that is, minimize playback interruptions, while maximizing video quality at a reasonable level of quality changes. In the case of live streaming, this task becomes particularly challenging due to the latency constraints. The challenge further increases if a client uses a wireless network, where the throughput is subject to considerable fluctuations. Consequently, live streams often exhibit latencies of up to 30 seconds. In the present work, we introduce an adaptation algorithm for HTTP-based live streaming called LOLYPOP (Low-Latency Prediction-Based Adaptation) that is designed to operate with a transport latency of few seconds. To reach this goal, LOLYPOP leverages TCP throughput predictions on multiple time scales, from 1 to 10 seconds, along with an estimate of the prediction error distribution. In addition to satisfying the latency constraint, the algorithm heuristically maximizes the quality of experience by maximizing the average video quality as a function of the number of skipped segments and quality transitions. In order to select an efficient prediction method, we studied the performance of several time series prediction methods in IEEE 802.11 wireless access networks. We evaluated LOLYPOP under a large set of experimental conditions limiting the transport latency to 3 seconds, against a state-of-the-art adaptation algorithm from the literature, called FESTIVE. We observed that the average video quality is by up to a factor of 3 higher than with FESTIVE. We also observed that LOLYPOP is able to reach a broader region in the quality of experience space, and thus it is better adjustable to the user profile or service provider requirements.Comment: Technical Report TKN-16-001, Telecommunication Networks Group, Technische Universitaet Berlin. This TR updated TR TKN-15-00

Energy-efficient wireless communication

In this chapter we present an energy-efficient highly adaptive network interface architecture and a novel data link layer protocol for wireless networks that provides Quality of Service (QoS) support for diverse traffic types. Due to the dynamic nature of wireless networks, adaptations in bandwidth scheduling and error control are necessary to achieve energy efficiency and an acceptable quality of service. In our approach we apply adaptability through all layers of the protocol stack, and provide feedback to the applications. In this way the applications can adapt the data streams, and the network protocols can adapt the communication parameters