1,049 research outputs found

    Graduate Catalog of Studies, 2023-2024

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    Graduate Catalog of Studies, 2023-2024

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    Beam scanning by liquid-crystal biasing in a modified SIW structure

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    A fixed-frequency beam-scanning 1D antenna based on Liquid Crystals (LCs) is designed for application in 2D scanning with lateral alignment. The 2D array environment imposes full decoupling of adjacent 1D antennas, which often conflicts with the LC requirement of DC biasing: the proposed design accommodates both. The LC medium is placed inside a Substrate Integrated Waveguide (SIW) modified to work as a Groove Gap Waveguide, with radiating slots etched on the upper broad wall, that radiates as a Leaky-Wave Antenna (LWA). This allows effective application of the DC bias voltage needed for tuning the LCs. At the same time, the RF field remains laterally confined, enabling the possibility to lay several antennas in parallel and achieve 2D beam scanning. The design is validated by simulation employing the actual properties of a commercial LC medium

    Analog Photonics Computing for Information Processing, Inference and Optimisation

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    This review presents an overview of the current state-of-the-art in photonics computing, which leverages photons, photons coupled with matter, and optics-related technologies for effective and efficient computational purposes. It covers the history and development of photonics computing and modern analogue computing platforms and architectures, focusing on optimization tasks and neural network implementations. The authors examine special-purpose optimizers, mathematical descriptions of photonics optimizers, and their various interconnections. Disparate applications are discussed, including direct encoding, logistics, finance, phase retrieval, machine learning, neural networks, probabilistic graphical models, and image processing, among many others. The main directions of technological advancement and associated challenges in photonics computing are explored, along with an assessment of its efficiency. Finally, the paper discusses prospects and the field of optical quantum computing, providing insights into the potential applications of this technology.Comment: Invited submission by Journal of Advanced Quantum Technologies; accepted version 5/06/202

    Development of electronics for microultrasound capsule endoscopy

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    Development of intracorporeal devices has surged in the last decade due to advancements in the semiconductor industry, energy storage and low-power sensing systems. This work aims to present a thorough systematic overview and exploration of the microultrasound (µUS) capsule endoscopy (CE) field as the development of electronic components will be key to a successful applicable µUSCE device. The research focused on investigating and designing high-voltage (HV, < 36 V) generating and driving circuits as well as a low-noise amplifier (LNA) for battery-powered and volume-limited systems. In implantable applications, HV generation with maximum efficiency is required to improve the operational lifetime whilst reducing the cost of the device. A fully integrated hybrid (H) charge pump (CP) comprising a serial-parallel (SP) stage was designed and manufactured for > 20 V and 0 - 100 µA output capabilities. The results were compared to a Dickson (DKCP) occupying the same chip area; further improvements in the SPCP topology were explored and a new switching scheme for SPCPs was introduced. A second regulated CP version was excogitated and manufactured to use with an integrated µUS pulse generator. The CP was manufactured and tested at different output currents and capacitive loads; its operation with an US pulser was evaluated and a novel self-oscillating CP mechanism to eliminate the need of an auxiliary clock generator with a minimum area overhead was devised. A single-output universal US pulser was designed, manufactured and tested with 1.5 MHz, 3 MHz, and 28 MHz arrays to achieve a means of fully-integrated, low-power transducer driving. The circuit was evaluated for power consumption and pulse generation capabilities with different loads. Pulse-echo measurements were carried out and compared with those from a commercial US research system to characterise and understand the quality of the generated pulse. A second pulser version for a 28 MHz array was derived to allow control of individual elements. The work involved its optimisation methodology and design of a novel HV feedback-based level-shifter. A low-noise amplifier (LNA) was designed for a wide bandwidth µUS array with a centre frequency of 28 MHz. The LNA was based on an energy-efficient inverter architecture. The circuit encompassed a full power-down functionality and was investigated for a self-biased operation to achieve lower chip area. The explored concepts enable realisation of low power and high performance LNAs for µUS frequencies

    Lux junior 2023: 16. Internationales Forum für den lichttechnischen Nachwuchs, 23. – 25. Juni 2023, Ilmenau : Tagungsband

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    Während des 16. Internationales Forums für den lichttechnischen Nachwuchs präsentieren Studenten, Doktoranden und junge Absolventen ihre Forschungs- und Entwicklungsergebnisse aus allen Bereichen der Lichttechnik. Die Themen bewegen sich dabei von Beleuchtungsanwendungen in verschiedensten Bereichen über Lichtmesstechnik, Kraftfahrzeugbeleuchung, LED-Anwendung bis zu nichtvisuellen Lichtwirkungen. Das Forum ist speziell für Studierende und junge Absolventen des Lichtbereiches konzipiert. Es bietet neben den Vorträgen und Postern die Möglichkeit zu Diskussionen und individuellem Austausch. In den 30 Jahren ihres Bestehens entwickelte sich die zweijährig stattfindende Tagung zu eine Traditionsveranstaltung, die das Fachgebiet Lichttechnik der TU Ilmenau gemeinsam mit der Bezirksgruppe Thüringen-Nordhessen der Deutschen Lichttechnischen Gesellschaft LiTG e. V. durchführt

    Analysis and Design of Silicon based Integrated Circuits for Radio Frequency Identification and Ranging Systems at 24GHz and 60GHz Frequency Bands

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    This scientific research work presents the analysis and design of radio frequency (RF) integrated circuits (ICs) designed for two cooperative RF identification (RFID) proof of concept systems. The first system concept is based on localizable and sensor-enabled superregenerative transponders (SRTs) interrogated using a 24GHz linear frequency modulated continuous wave (LFMCW) secondary radar. The second system concept focuses on low power components for a 60GHz continuous wave (CW) integrated single antenna frontend for interrogating close range passive backscatter transponders (PBTs). In the 24GHz localizable SRT based system, a LFMCW interrogating radar sends a RF chirp signal to interrogate SRTs based on custom superregenerative amplifier (SRA) ICs. The SRTs receive the chirp and transmit it back with phase coherent amplification. The distance to the SRTs are then estimated using the round trip time of flight method. Joint data transfer from the SRT to the interrogator is enabled by a novel SRA quench frequency shift keying (SQ-FSK) based low data rate simplex communication. The SRTs are also designed to be roll invariant using bandwidth enhanced microstrip patch antennas. Theoretical analysis is done to derive expressions as a function of system parameters including the minimum SRA gain required for attaining a defined range and equations for the maximum number of symbols that can be transmitted in data transfer mode. Analysis of the dependency of quench pulse characteristics during data transfer shows that the duty cycle has to be varied while keeping the on-time constant to reduce ranging errors. Also the worsening of ranging precision at longer distances is predicted based on the non-idealities resulting from LFMCWchirp quantization due to SRT characteristics and is corroborated by system level measurements. In order to prove the system concept and study the semiconductor technology dependent factors, variants of 24GHz SRA ICs are designed in a 130nm silicon germanium (SiGe) bipolar complementary metal oxide technology (BiCMOS) and a partially depleted silicon on insulator (SOI) technology. Among the SRA ICs designed, the SiGe-BiCMOS ICs feature a novel quench pulse shaping concept to simultaneously improve the output power and minimum detectable input power. A direct antenna drive SRA IC based on a novel stacked transistor cross-coupled oscillator topology employing this concept exhibit one of the best reported combinations of minimum detected input power level of −100 dBm and output power level of 5.6 dBm, post wirebonding. The SiGe stacked transistor with base feedback capacitance topology employed in this design is analyzed to derive parameters including the SRA loop gain for design optimization. Other theoretical contributions include the analysis of the novel integrated quench pulse shaping circuit and formulas derived for output voltage swing taking bondwire losses into account. Another SiGe design variant is the buffered antenna drive SRA IC having a measured minimum detected input power level better than −80 dBm, and an output power level greater than 3.2 dBm after wirebonding. The two inputs and outputs of this IC also enables the design of roll invariant SRTs. Laboratory based ranging experiments done to test the concepts and theoretical considerations show a maximum measured distance of 77m while transferring data at the rate of 0.5 symbols per second using SQ-FSK. For distances less than 10m, the characterized accuracy is better than 11 cm and the precision is better than 2.4 cm. The combination of the maximum range, precision and accuracy are one of the best reported among similar works in literature to the author’s knowledge. In the 60GHz close range CW interrogator based system, the RF frontend transmits a continuous wave signal through the transmit path of a quasi circulator (QC) interfaced to an antenna to interrogate a PBT. The backscatter is received using the same antenna interfaced to the QC. The received signal is then amplified and downconverted for further processing. To prove this concept, two optimized QC ICs and a downconversion mixer IC are designed in a 22nm fully depleted SOI technology. The first QC is the transmission lines based QC which consumes a power of 5.4mW, operates at a frequency range from 56GHz to 64GHz and occupies an area of 0.49mm2. The transmit path loss is 5.7 dB, receive path gain is 2 dB and the tunable transmit path to receive path isolation is between 20 dB and 32 dB. The second QC is based on lumped elements, and operates in a relatively narrow bandwidth from 59.6GHz to 61.5GHz, has a gain of 8.5 dB and provides a tunable isolation better than 20 dB between the transmit and receive paths. This QC design also occupies a small area of 0.34mm² while consuming 13.2mW power. The downconversion is realized using a novel folded switching stage down conversion mixer (FSSDM) topology optimized to achieve one of the best reported combination of maximum voltage conversion gain of 21.5 dB, a factor of 2.5 higher than reported state-of-the-art results, and low power consumption of 5.25mW. The design also employs a unique back-gate tunable intermediate frequency output stage using which a gain tuning range of 5.5 dB is attained. Theoretical analysis of the FSSDM topology is performed and equations for the RF input stage transconductance, bandwidth, voltage conversion gain and gain tuning are derived. A feasibility study for the components of the 60GHz integrated single antenna interrogator frontend is also performed using PBTs to prove the system design concept.:1 Introduction 1 1.1 Motivation and Related Work . . . . . . . . . . . . . . . . . . . . . 1 1.2 Scope and Functional Specifications . . . . . . . . . . . . . . . . . 4 1.3 Objectives and Structure . . . . . . . . . . . . . . . . . . . . . . . . 5 2 Features and Fundamentals of RFIDs and Superregenerative Amplifiers 9 2.1 RFID Transponder Technology . . . . . . . . . . . . . . . . . . . . 9 2.1.1 Chipless RFID Transponders . . . . . . . . . . . . . . . . . 10 2.1.2 Semiconductor based RFID Transponders . . . . . . . . . . 11 2.1.2.1 Passive Transponders . . . . . . . . . . . . . . . . 11 2.1.2.2 Active Transponders . . . . . . . . . . . . . . . . . 13 2.2 RFID Interrogator Architectures . . . . . . . . . . . . . . . . . . . 18 2.2.1 Interferometer based Interrogator . . . . . . . . . . . . . . . 19 2.2.2 Ultra-wideband Interrogator . . . . . . . . . . . . . . . . . . 20 2.2.3 Continuous Wave Interrogators . . . . . . . . . . . . . . . . 21 2.3 Coupling Dependent Range and Operating Frequencies . . . . . . . 25 2.4 RFID Ranging Techniques . . . . . . . . . . . . . . . . . . . . . . . 28 2.4.0.1 Received Signal Strength based Ranging . . . . . 28 2.4.0.2 Phase based Ranging . . . . . . . . . . . . . . . . 30 2.4.0.3 Time based Ranging . . . . . . . . . . . . . . . . . 30 2.5 Architecture Selection for Proof of Concept Systems . . . . . . . . 32 2.6 Superregenerative Amplifier (SRA) . . . . . . . . . . . . . . . . . . 35 2.6.1 Fundamentals . . . . . . . . . . . . . . . . . . . . . . . . . . 35 2.6.2 Modes of Operation . . . . . . . . . . . . . . . . . . . . . . 42 2.6.3 Frequency Domain Characteristics . . . . . . . . . . . . . . 45 2.7 Semiconductor Technologies for RFIC Design . . . . . . . . . . . . 48 2.7.1 Silicon Germanium BiCMOS . . . . . . . . . . . . . . . . . 48 2.7.2 Silicon-on-Insulator . . . . . . . . . . . . . . . . . . . . . . . 48 3 24GHz Superregenerative Transponder based Identification and Rang- ing System 51 3.1 System Design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 3.1.1 SRT Identification and Ranging . . . . . . . . . . . . . . . . 51 3.1.2 Power Link Analysis . . . . . . . . . . . . . . . . . . . . . . 55 3.1.3 Non-idealities . . . . . . . . . . . . . . . . . . . . . . . . . . 59 3.1.4 SRA Quench Frequency Shift Keying for data transfer . . . 61 3.1.5 Knowledge Gained . . . . . . . . . . . . . . . . . . . . . . . 63 3.2 RFIC Designs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 3.2.1 Low Power Direct Antenna Drive CMOS SRA IC . . . . . . 66 3.2.1.1 Circuit analysis and design . . . . . . . . . . . . . 66 3.2.1.2 Characterization . . . . . . . . . . . . . . . . . . . 69 3.2.2 Direct Antenna Drive SiGe SRA ICs . . . . . . . . . . . . . 71 3.2.2.1 Stacked Transistor Cross-coupled Quenchable Oscillator . . . . . . . . . . . . . . . . . . . . . . . . 72 3.2.2.1.1 Resonator . . . . . . . . . . . . . . . . . . 72 3.2.2.1.2 Output Network . . . . . . . . . . . . . . 75 3.2.2.1.3 Stacked Transistor Cross-coupled Pair and Loop Gain . . . . . . . . . . . . . . . . . 77 3.2.2.2 Quench Waveform Design . . . . . . . . . . . . . . 85 3.2.2.3 Characterization . . . . . . . . . . . . . . . . . . . 89 3.2.3 Antenna Diversity SiGe SRA IC with Integrated Quench Pulse Shaping . . . . . . . . . . . . . . . . . . . . . . . . . . 91 3.2.3.1 Circuit Analysis and Design . . . . . . . . . . . . 91 3.2.3.1.1 Crosscoupled Pair and Sampling Current 94 3.2.3.1.2 Common Base Input Stage . . . . . . . . 95 3.2.3.1.3 Cascode Output Stage . . . . . . . . . . . 96 3.2.3.1.4 Quench Pulse Shaping Circuit . . . . . . 96 3.2.3.1.5 Power Gain . . . . . . . . . . . . . . . . . 99 3.2.3.2 Characterization . . . . . . . . . . . . . . . . . . . 102 3.2.4 Knowledge Gained . . . . . . . . . . . . . . . . . . . . . . . 103 3.3 Proof of Principle System Implementation . . . . . . . . . . . . . . 106 3.3.1 Superregenerative Transponders . . . . . . . . . . . . . . . 106 3.3.1.1 Bandwidth Enhanced Microstrip Patch Antennas 108 3.3.2 FMCW Radar Interrogator . . . . . . . . . . . . . . . . . . 114 3.3.3 Chirp Z-transform Based Data Analysis . . . . . . . . . . . 116 4 60GHz Single Antenna RFID Interrogator based Identification System 121 4.1 System Design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 4.2 RFIC Designs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125 4.2.1 Quasi-circulator ICs . . . . . . . . . . . . . . . . . . . . . . 125 4.2.1.1 Transmission Lines based Quasi-Circulator IC . . 126 4.2.1.2 Lumped Elements WPD based Quasi-Circulator . 130 4.2.1.3 Characterization . . . . . . . . . . . . . . . . . . . 134 4.2.1.4 Knowledge Gained . . . . . . . . . . . . . . . . . . 135 4.2.2 Folded Switching Stage Downconversion Mixer IC . . . . . 138 4.2.2.1 FSSDM Circuit Design . . . . . . . . . . . . . . . 138 4.2.2.2 Cascode Transconductance Stage . . . . . . . . . . 138 4.2.2.3 Folded Switching Stage with LC DC Feed . . . . . 142 4.2.2.4 LO Balun . . . . . . . . . . . . . . . . . . . . . . . 145 4.2.2.5 Backgate Tunable IF Stage and Offset Correction 146 4.2.2.6 Voltage Conversion Gain . . . . . . . . . . . . . . 147 4.2.2.7 Characterization . . . . . . . . . . . . . . . . . . . 150 4.2.2.8 Knowledge Gained . . . . . . . . . . . . . . . . . . 151 4.3 Proof of Principle System Implementation . . . . . . . . . . . . . . 154 5 Experimental Tests 157 5.1 24GHz System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 5.1.1 Ranging Experiments . . . . . . . . . . . . . . . . . . . . . 157 5.1.2 Roll Invariance Experiments . . . . . . . . . . . . . . . . . . 158 5.1.3 Joint Ranging and Data Transfer Experiments . . . . . . . 158 5.2 60GHz System Detection Experiments . . . . . . . . . . . . . . . . 165 6 Summary and Future Work 167 Appendices 171 A Derivation of Parameters for CB Amplifier with Base Feedback Capac- itance 173 B Definitions 177 C 24GHz Experiment Setups 179 D 60 GHz Experiment Setups 183 References 185 List of Original Publications 203 List of Abbreviations 207 List of Symbols 213 List of Figures 215 List of Tables 223 Curriculum Vitae 22

    Arbitrarily Tunable Phase Shift in Low-Frequency Multiphase Oscillator

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    A special electronically tunable multiphase oscillator with arbitrarily and continuously adjustable phase shifts is introduced. Our design assumes to set the phase around the asymptotical limit of 180.. These features cannot be easily achieved in a standard way, i.e., any simple single-phase oscillator supplemented by a first-order adjustable all-pass (AP) section (shifter). The proposed design uses an electronically linearly tunable quadrature oscillator with a frequency range from 0.98 up to 12.54 kHz. It also offers multiples of 45. as the initial setting of the phase shift tuning region. The example of operation shows the adjustment of the phase shift at a specific frequency (10 kHz) within the range of +/- 45 degrees. and around -180 degrees, -135 degrees, and -90 degrees. This variability is not available in standard cases without the use of several AP sections. The current value of the phase shift of the presented oscillator is electronically controlled and does not influence the oscillation frequency and condition of oscillation. Output levels of produced signals are not influenced by this tuning process and are in the range of several hundreds of mV. Two applications of the oscillator are proposed. The first one focuses on low-bitrate modulation systems [phase shift keying (PSK)] while in the second one, our circuit represents a source of phase-adjustable signals in acoustic experiments. Discrete passive elements and active devices (special multipliers having current output terminals, unity-gain differential voltage buffers) fabricated in 0.35 mu m I3T25 ON Semiconductor 3.3 V CMOS process are used in experimental verification

    Electronic Devices for the Combination of Electrically Controlled Drug Release, Electrostimulation, and Optogenetic Stimulation for Nerve Tissue Regeneration

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    [ES] La capacidad de las células madre para proliferar formando distintas células especializadas les otorga la potencialidad de servir de base para terapias efectivas para patologías cuyo tratamiento era inimaginable hasta hace apenas dos décadas. Sin embargo, esta capacidad se encuentra mediada por estímulos fisiológicos, químicos, y eléctricos, específicos y complejos, que dificultan su traslación a la rutina clínica. Por ello, las células madre representan un campo de estudio en el que se invierten amplios esfuerzos por parte de la comunidad científica. En el ámbito de la regeneración nerviosa, para modular su desarrollo y diferenciación el tratamiento farmacológico, la electroestimulación, y la estimulación optogenética son técnicas que están consiguiendo prometedores resultados. Es por ello por lo que en la presente tesis se ha desarrollado un conjunto de sistemas electrónicos para permitir la aplicación combinada de estas técnicas in vitro, con perspectiva a su aplicación in vivo. Hemos diseñado una novedosa tecnología para la liberación eléctricamente controlada de fármacos. Esta tecnología está basada en nanopartículas de sílice mesoporosa y puertas moleculares de bipiridina-heparina. Las puertas moleculares son electroquímicamente reactivas, y encierran los fármacos en el interior de las nanopartículas, liberándolos ante un estímulo eléctrico. Hemos caracterizado esta tecnología, y la hemos validado mediante la liberación controlada de rodamina en cultivos celulares de HeLa. Para la combinación de liberación controlada de fármacos y electroestimulación hemos desarrollado dispositivos que permiten aplicar los estímulos eléctricos de forma configurable desde una interfaz gráfica de usuario. Además, hemos diseñado un módulo de expansión que permite multiplexar las señales eléctricas a diferentes cultivos celulares. Además, hemos diseñado un dispositivo de estimulación optogenética. Este tipo de estimulación consiste en la modificación genética de las células para que sean sensibles a la radiación lumínica de determinada longitud de onda. En el ámbito de la regeneración de tejido mediante células precursoras neurales, es de interés poder inducir ondas de calcio, favoreciendo su diferenciación en neuronas y la formación de circuitos sinápticos. El dispositivo diseñado permite obtener imágenes en tiempo real mediante microscopía confocal de las respuestas transitorias de las células al ser irradiadas. El dispositivo se ha validado irradiando neuronas modificadas con luz pulsada de 100 ms. También hemos diseñado un dispositivo electrónico complementario de medida de irradiancia con el doble fin de permitir la calibración del equipo de irradiancia y medir la irradiancia en tiempo real durante los experimentos in vitro. Los resultados del uso de los bioactuadores en procesos complejos y dinámicos, como la regeneración de tejido nervioso, son limitados en lazo abierto. Uno de los principales aspectos analizados es el desarrollo de biosensores que permitiesen la cuantización de ciertas biomoléculas para ajustar la estimulación suministrada en tiempo real. Por ejemplo, la segregación de serotonina es una respuesta identificada en la elongación de células precursoras neurales, pero hay otras biomoléculas de interés para la implementación de un control en lazo cerrado. Entre las tecnologías en el estado del arte, los biosensores basados en transistores de efecto de campo (FET) funcionalizados con aptámeros son realmente prometedores para esta aplicación. Sin embargo, esta tecnología no permitía la medición simultánea de más de una biomolécula objetivo en un volumen reducido debido a las interferencias entre los distintos FETs, cuyos terminales se encuentran inmersos en la solución. Por ello, hemos desarrollado instrumentación electrónica capaz de medir simultáneamente varios de estos biosensores, y la hemos validado mediante la medición simultánea de pH y la detección preliminar de serotonina y glutamato.[CA] La capacitat de les cèl·lules mare per a proliferar formant diferents cèl·lules especialitzades els atorga la potencialitat de servir de base per a teràpies efectives per a patologies el tractament de les quals era inimaginable fins fa a penes dues dècades. No obstant això, aquesta capacitat es troba mediada per estímuls fisiològics, químics, i elèctrics, específics i complexos, que dificulten la seua translació a la rutina clínica. Per això, les cèl·lules mare representen un camp d'estudi en el qual s'inverteixen amplis esforços per part de la comunitat científica. En l'àmbit de la regeneració nerviosa, per a modular el seu desenvolupament i diferenciació el tractament farmacològic, l'electroestimulació, i l'estimulació optogenética són tècniques que estan aconseguint prometedors resultats. És per això que en la present tesi s'ha desenvolupat un conjunt de sistemes electrònics per a permetre l'aplicació combinada d'aquestes tècniques in vitro, amb perspectiva a la seua aplicació in vivo. Hem dissenyat una nova tecnologia per a l'alliberament elèctricament controlat de fàrmacs. Aquesta tecnologia està basada en nanopartícules de sílice mesoporosa i portes moleculars de bipiridina-heparina. Les portes moleculars són electroquímicament reactives, i tanquen els fàrmacs a l'interior de les nanopartícules, alliberant-los davant un estímul elèctric. Hem caracteritzat aquesta tecnologia, i l'hem validada mitjançant l'alliberament controlat de rodamina en cultius cel·lulars de HeLa. Per a la combinació d'alliberament controlat de fàrmacs i electroestimulació hem desenvolupat dispositius que permeten aplicar els estímuls elèctrics de manera configurable des d'una interfície gràfica d'usuari. A més, hem dissenyat un mòdul d'expansió que permet multiplexar els senyals elèctrics a diferents cultius cel·lulars. A més, hem dissenyat un dispositiu d'estimulació optogenètica. Aquest tipus d'estimulació consisteix en la modificació genètica de les cèl·lules perquè siguen sensibles a la radiació lumínica de determinada longitud d'ona. En l'àmbit de la regeneració de teixit mitjançant cèl·lules precursores neurals, és d'interés poder induir ones de calci, afavorint la seua diferenciació en neurones i la formació de circuits sinàptics. El dispositiu dissenyat permet obtindré imatges en temps real mitjançant microscòpia confocal de les respostes transitòries de les cèl·lules en ser irradiades. El dispositiu s'ha validat irradiant neurones modificades amb llum polsada de 100 ms. També hem dissenyat un dispositiu electrònic complementari de mesura d'irradiància amb el doble fi de permetre el calibratge de l'equip d'irradiància i mesurar la irradiància en temps real durant els experiments in vitro. Els resultats de l'ús dels bioactuadors en processos complexos i dinàmics, com la regeneració de teixit nerviós, són limitats en llaç obert. Un dels principals aspectes analitzats és el desenvolupament de biosensors que permeteren la quantització de certes biomolècules per a ajustar l'estimulació subministrada en temps real. Per exemple, la segregació de serotonina és una resposta identificada amb l'elongació de les cèl·lules precursores neurals, però hi ha altres biomolècules d'interés per a la implementació d'un control en llaç tancat. Entre les tecnologies en l'estat de l'art, els biosensors basats en transistors d'efecte de camp (FET) funcionalitzats amb aptàmers són realment prometedors per a aquesta aplicació. No obstant això, aquesta tecnologia no permetia el mesurament simultani de més d'una biomolècula objectiu en un volum reduït a causa de les interferències entre els diferents FETs, els terminals dels quals es troben immersos en la solució. Per això, hem desenvolupat instrumentació electrònica capaç de mesurar simultàniament diversos d'aquests biosensors i els hem validat amb mesurament simultani del pH i la detecció preliminar de serotonina i glutamat.[EN] The stem cells' ability to proliferate to form different specialized cells gives them the potential to serve as the basis for effective therapies for pathologies whose treatment was unimaginable until just two decades ago. However, this capacity is mediated by specific and complex physiological, chemical, and electrical stimuli that complicate their translation to clinical routine. For this reason, stem cells represent a field of study in which the scientific community is investing a great deal of effort. In the field of nerve regeneration, to modulate their development and differentiation, pharmacological treatment, electrostimulation, and optogenetic stimulation are techniques that are achieving promising results. For this reason, we have developed a set of electronic systems to allow the combined application of these techniques in vitro, with a view to their application in vivo. We have designed a novel technology for the electrically controlled release of drugs. This technology is based on mesoporous silica nanoparticles and bipyridine-heparin molecular gates. The molecular gates are electrochemically reactive and entrap the drugs inside the nanoparticles, releasing them upon electrical stimulus. We have characterized this technology and validated it by controlled release of rhodamine in HeLa cell cultures. For combining electrostimulation and controlled drug release we have developed devices that allow applying the different electrical stimuli in a configurable way from a graphical user interface. In addition, we have designed an expansion module that allows multiplexing electrical signals to different cell cultures. In addition, we have designed an optogenetic stimulation device. This type of stimulation consists of genetically modifying cells to make them sensitive to light radiation of a specific wavelength. In tissue regeneration using neural precursor cells, it is interesting to be able to induce calcium waves, favoring the cell differentiation into neurons and the formation of synaptic circuits. The designed device enable the obtention of real-time images through confocal microscopy of the transient responses of cells upon irradiation. The device has been validated by irradiating modified neurons with 100 ms pulsed light stimulation. We have also designed a complementary electronic irradiance measurement device to allow calibration of the irradiator equipment and measuring irradiance in real time during in vitro experiments. The results of using bioactuators in complex and dynamic processes, such as nerve tissue regeneration, are limited in an open loop. One of the main aspects analyzed is the development of biosensors that would allow quantifying of specific biomolecules to adjust the stimulation provided in real time. For instance, serotonin secretion is an identified response of neural precursor cells elongation, among other biomolecules of interest for the implementation of a closed-loop control. Among the state-of-the-art technologies, biosensors based on field effect transistors (FETs) functionalized with aptamers are promising for this application. However, this technology did not allow the simultaneous measurement of more than one target biomolecule in a small volume due to interferences between the different FETs, whose terminals are immersed in the solution. This is why we have developed electronic instrumentation capable of simultaneously measuring several of these biosensors, and we have validated it with the simultaneous pH measurement and the preliminary detection of serotonin and glutamate.Monreal Trigo, J. (2023). Electronic Devices for the Combination of Electrically Controlled Drug Release, Electrostimulation, and Optogenetic Stimulation for Nerve Tissue Regeneration [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/19384

    Design, Fabrication and Characterization of Capacitively Coupled Silicon-Organic Hybrid Modulators

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    Silicon-organic hybrid (SOH) modulators [1] offer fast and efficient electro-optic modulation with very small footprints. Traditional SOH modulators suffer from bandwidth limitation due to the higher RC time constant that originates from the resistive coupling of the RF signal. In the current thesis, we propose a new modulator concept establishing a capacitive coupling of the RF signal as opposed to the resistive one in the conventional SOH modulators and hence the name capacitively coupled SOH (CC-SOH) modulator. A high-κ dielectric, BaT iO3 is characterized in optical and in RF regime and later used to design capacitively coupled SOH modulator. The fabicated CCSOH is characterized to have a flat frequency response up to 65 GHz indicating that the 3-dB bandwidth is at least 3 times higher than the SOH modulators
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