21,634 research outputs found

    Anu√°rio cient√≠fico da Escola Superior de Tecnologia da Sa√ļde de Lisboa - 2021

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    √Č com grande prazer que apresentamos a mais recente edi√ß√£o (a 11.¬™) do Anu√°rio Cient√≠fico da Escola Superior de Tecnologia da Sa√ļde de Lisboa. Como institui√ß√£o de ensino superior, temos o compromisso de promover e incentivar a pesquisa cient√≠fica em todas as √°reas do conhecimento que contemplam a nossa miss√£o. Esta publica√ß√£o tem como objetivo divulgar toda a produ√ß√£o cient√≠fica desenvolvida pelos Professores, Investigadores, Estudantes e Pessoal n√£o Docente da ESTeSL durante 2021. Este Anu√°rio √©, assim, o reflexo do trabalho √°rduo e dedicado da nossa comunidade, que se empenhou na produ√ß√£o de conte√ļdo cient√≠fico de elevada qualidade e partilhada com a Sociedade na forma de livros, cap√≠tulos de livros, artigos publicados em revistas nacionais e internacionais, resumos de comunica√ß√Ķes orais e p√≥steres, bem como resultado dos trabalhos de 1¬ļ e 2¬ļ ciclo. Com isto, o conte√ļdo desta publica√ß√£o abrange uma ampla variedade de t√≥picos, desde temas mais fundamentais at√© estudos de aplica√ß√£o pr√°tica em contextos espec√≠ficos de Sa√ļde, refletindo desta forma a pluralidade e diversidade de √°reas que definem, e tornam √ļnica, a ESTeSL. Acreditamos que a investiga√ß√£o e pesquisa cient√≠fica √© um eixo fundamental para o desenvolvimento da sociedade e √© por isso que incentivamos os nossos estudantes a envolverem-se em atividades de pesquisa e pr√°tica baseada na evid√™ncia desde o in√≠cio dos seus estudos na ESTeSL. Esta publica√ß√£o √© um exemplo do sucesso desses esfor√ßos, sendo a maior de sempre, o que faz com que estejamos muito orgulhosos em partilhar os resultados e descobertas dos nossos investigadores com a comunidade cient√≠fica e o p√ļblico em geral. Esperamos que este Anu√°rio inspire e motive outros estudantes, profissionais de sa√ļde, professores e outros colaboradores a continuarem a explorar novas ideias e contribuir para o avan√ßo da ci√™ncia e da tecnologia no corpo de conhecimento pr√≥prio das √°reas que comp√Ķe a ESTeSL. Agradecemos a todos os envolvidos na produ√ß√£o deste anu√°rio e desejamos uma leitura inspiradora e agrad√°vel.info:eu-repo/semantics/publishedVersio

    Metagenomic assessment of nitrate-contaminated mine wastewaters and optimization of complete denitrification by indigenous enriched bacteria

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    Nitrate contamination in water remains to be on the rise globally due to continuous anthropogenic activities, such as mining and farming, which utilize high amounts of ammonium nitrate explosives and chemical-NPK-fertilizers, respectively. This study presents insights into the development of a bioremediation strategy to remove nitrates (NO3‚ąí) using consortia enriched from wastewater collected from a diamond mine in Lesotho and a platinum mine in South Africa. A biogeochemical analysis was conducted on the water samples which aided in comparing and elucidating their unique physicochemical parameters. The chemical analysis uncovered that both wastewater samples contained over 120¬†mg/L of NO3‚ąí and over 250¬†mg/L of sulfates (SO42-), which were both beyond the acceptable limit of the environmental surface water standards of South Africa. The samples were atypical of mine wastewaters as they had low concentrations of dissolved heavy metals and a pH of over 5. A metagenomic analysis applied to study microbial diversities revealed that both samples were dominated by the phyla Proteobacteria and Bacteroidetes, which accounted for over 40% and 15%, respectively. Three consortia were enriched to target denitrifying bacteria using selective media and then subjected to complete denitrification experiments. Denitrification dynamics and denitrifying capacities of the consortia were determined by monitoring dissolved and gaseous nitrogen species over time. Denitrification optimization was carried out by changing environmental conditions, including supplementing the cultures with metal enzyme co-factors (iron and copper) that were observed to promote different stages of denitrification. Copper supplemented at 50¬†mg/L was observed to be promoting complete denitrification of over 500¬†mg/L of NO3‚ąí, evidenced by the emission of nitrogen gas (N2) that was more than nitrous oxide gas (N2O) emitted as the terminal by-product. Modification and manipulation of growth conditions based on the microbial diversity enriched proved that it is possible to optimize a bioremediation system that can reduce high concentrations of NO3‚ąí, while emitting an environmentally-friendly N2 instead of N2O, that is, a greenhouse gas. Data collected and discussed in this research study can be used to model an upscale NO3‚ąí bioremediation system aimed to remove nitrogenous and other contaminants without secondary contamination

    Effects of replacing dietary fish oil and fish meal with microbial oil and algal biomass on lipid class, total fatty acid and phospholipid fatty acid composition of Atlantic salmon liver and muscle tissues

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    With the continuous growth of aquaculture comes a growing demand for an alternative lipid source for fish oil (FO) and fish meal (FM) in aquafeeds. Certain microorganisms provide a potential sustainable replacement for FO and FM due to their content of omega-3 (ŌČ3) long-chain polyunsaturated fatty acids, which are essential for the growth and health of fish. Two feeding trials were conducted to determine the effects of replacing FO and FM with oil and biomass from two different microorganism species. The first feeding trial comprised of replacing FO with a microbial oil (MO) derived from a novel strain, Schizochytrium sp. (strain T18), in diets for Atlantic salmon. Four experimental diets were developed: a fish oil control diet (FO), a blend of fish oil/canola oil control diet (FO/CO), a fish oil replacement with low proportions of microbial oil diet (LMO), and a fish oil replacement with high proportions of microbial oil diet (HMO). After 16 weeks of feeding, there were no significant effects on growth parameters across the dietary treatments. Fatty acid profiles reflected the diets with DHA being present in high proportions in the tissues, especially in the cellular membrane. The purpose of the second feeding trial was to reduce FO and replacing FM with algal biomass (AB) derived from Pavlova sp. strain CCMP459 (Pav459) in diets for Atlantic salmon. Three experimental diets were developed: a fish meal control diet (FM), a blend of a fish meal/algal biomass Pav459 diet (FM/AB), and a complete FM replacement with algal biomass Pav459 diet (AB). After 12 weeks of feeding, again, there was no significant effect on growth paraments, and the fatty acid profiles also reflected those of the diets. The DHA was present in high proportions in the tissues for all dietary treatments, especially the cellular membrane. The stable isotope data suggested a direct integration of EPA and DHA and not biosynthesis from its precursor ALA

    An investigation of the geothermal potential of the Upper Devonian sandstones beneath eastern Glasgow

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    The urban development of the city of Glasgow is a consequence of its economic development, in part fuelled by local coalfields which exploited rocks in the same sedimentary basin within which geothermal resources in flooded abandoned mine workings, and deeper hot sedimentary aquifers (HSA), are present. This creates an opportunity to provide geothermal heating to areas of dense urban population with high heat demand. The depth of the target HSA geothermal resource, in Upper Devonian aged sandstones of the Stratheden Group, beneath eastern Glasgow was determined by gravity surveying and structural geological modelling. The estimated depth of the geothermal resource ranged from c.1500-2000 m, in the eastward deepening sedimentary basin. To reliably estimate the temperature of the geothermal resource, rigorous corrections to account for the effects of palaeoclimate and topography on heat flow were applied to boreholes in the Greater Glasgow area. The mean regional corrected heat flow was calculated as 75.7 mW m-2, an increase of 13.8 mW m-2 from the uncorrected value of 61.9 mW m-2, emphasising the extent to which heat flow was previously underestimated. Extrapolation of the geothermal gradient, calculated from the mean regional corrected heat flow, results in aquifer temperatures of c. 64-79 ¬įC at depths of c.1500-2000 m beneath eastern Glasgow. The geothermal resource may, therefore, be capable of supporting a wide variety of direct heat use applications if sufficient matrix permeability or fracture networks are present. However, diagenetic effects such as quartz and carbonate cementation were found to restrict the porosity in Upper Devonian sandstones in a borehole and outcrop analogue study. These effects may likewise reduce porosity and intergranular permeability in the target aquifer, although this crucial aspect cannot be fully understood without deep exploratory drilling. To quantify the magnitude of the deep geothermal resource, the indicative thermal power outputs of geothermal doublet wells located in Glasgow‚Äôs East End were calculated for the first time, with outputs ranging from 1.3-2.1 MW dependent upon the aquifer depth. This, however, is predicated upon an aquifer permeability of c. 40 mD, which if reduced to 10 mD or less due to the effects of diagenesis, significantly reduces the thermal power outputs to 230-390 kW. The lack of assured project-success, given uncertainties related to the aquifer properties at depth, coupled with high capital costs of drilling, pose barriers to the development of deep geothermal energy in Glasgow. Further investigation of the economic viability of geothermal exploration, and alternative technological solutions is therefore required to mitigate the technical and economic risks. However, if sufficient matrix permeability or fracture networks are present at depth in the Upper Devonian sandstone sequence, then the potential contribution that geothermal energy could make to meeting local heat demand, reducing greenhouse gas emissions, and addressing the ‚Äėenergy trilemma‚Äô in Glasgow is significant

    Management controls, government regulations, customer involvement: Evidence from a Chinese family-owned business

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    This research reports on a case study of a family-owned elevator manufacturing company in China, where management control was sandwiched between the state policies and global customer production requirements. By analysing the role of government and customer, this thesis aimed to illustrate how management control operated in a family-owned business and to see how and why they do management control differently. In particular, it focused on how international production standards and existing Chinese industry policies translated into a set of the management control practices through a local network within the family-owned business I studied. Based on an ethnographic approach to research, I spent six months in the field, conducted over 30 interviews, several conservations, and reviewed relevant internal documents to understand how management control (MC) techniques with humans cooperated in the company. I also understood how two layers of pressure have shaped company behaviour, and how a company located in a developing country is connecting with global network. I also found there is considerable tension among key actors and investigated how the company responded and managed it. Drawing on Actor Network Theory (ANT), I analysed the interviews from key actors, examined the role of government regulations and customer requirements to see how management control being managed under two layers of pressure, i.e., the government regulations (e.g., labour, tax, environment control) and customer requirement (e.g., quality and production control). Management controls were an obligatory passage point (OPP), and transformation of those elements of Western production requirements and government requirements arrived at the Chinese local factory and influenced management control and budgeting. The findings suggest that management control systems are not only a set of technical procedures, but it is also about managing tensions. This understanding shows a linear perspective on MC practices rather than a social perspective. However, when we use ANT as a theoretical perspective, we see those actors who, being obliged and sandwiched, and controlled by external forces for them to follow. Consequently, human actors must work in an unavoidable OPP. This is the tension they face which constructed mundane practices of MC. Hence, MCs are managing such tensions. This study contributes to management control research by analysing management controls in terms of OPP, extends our understanding by illustrating the role of the government and customers, and our understanding of family-owned business from a management controls perspective in a developing country

    Material Economies of South Yorkshire. The Organisation of Metal Production in Roman South Yorkshire.

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    This thesis aims to develop a model for the social organisation and production of ferrous and non-ferrous metals in South Yorkshire during the Roman period. This characterisation of the organisation of metallurgical activities is achieved through a combined methodology that will gather data from grey literature, published literature, as well as chemical, visual and microstructural analysis of metallurgical debris. The metallurgical practices in the study area are primarily rural in nature. These results are looked at through the lenses of Agency, Habitus, and the social construction of craft production. The movement of materials and people within the study area and local specialist practices are central in the interpretation of regional metalworking practices. Furthermore, models of craft production are critiqued, and an alternative modelisation process is suggested to characterise and understand the organisation of metal production in Roman South Yorkshire

    The Isolation and Characterization of Bacteriophage Hasitha

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    Microbacteriophage Hasitha is a virus that infects Microbacterium foliorum, a bacterium associated with grasses that was first discovered in Germany. Hasitha was isolated from an enriched compost sample and is of particular interest due to its unusual growth pattern. Most bacteriophages require actively growing host cells to produce new phage progeny. However, Hasitha can infect and kill stationary (non-replicating) bacterial cells. We discovered this unusual characteristic through a fortuitous observation of infected lawns that were allowed to incubate in the lab workspace for approximately one month. During this time, a noticeable ‚Äúhalo‚ÄĚ grew around the initial site of infection and consumed most of the lawn. Here we report the genomic sequence of Hasitha and its ultrastructural features. We also report the results of experiments to determine the mechanism for the expanding halos of growth. Our results suggest that Hasitha phage particles diffuse outward from the initial site of infection and continue to infect and lyse surrounding stationary host cells. We also found through analysis of bacteriophages closely related to Hasitha that the halos are genetic in nature, with genome annotation suggesting a few gene products of unknown function to have possibly hydrolytic properties. Lastly, thin sectioning determined that the bacterial hosts found in the developed halo are no longer in stationary phase. These findings elucidate previously unknown factors in phage infection of stationary phase bacteria and provide data to be expanded upon in future studies
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