RF Sensors for Monitoring the Electrical Properties of Electrolyte Solutions

A radio frequency electrical sensor for the qualitative analysis and monitoring of the electrical properties of electrolyte solutions is designed, simulated and experimentally tested in this research. This work is based on the use of planar inductors for the detection of a change in the concentration of ionic species in a liquid sample. At first a literature review on the physical chemistry of electrolyte solutions is provided. This will include topics on the conductivity and relaxation properties of electrolytes. This will be followed by a look at dielectric spectroscopy sensors, electrochemical sensors and inductive sensing devices. The principles of electrodynamics and constitutive equations are discussed. Based on these, the principles of operation of the RF electrical sensors are analysed. Two methods of theoretical analysis of such structures are investigated. These methods are; analytical solution and finite element computation method. The former offers greater insight into the system’s parameters whilst the latter offers more information regarding the whole system. Given the qualitative nature of the sensors under investigation and finite element approach was selected and used in latter chapters to obtain grater insight into the behaviour of the system. Planar inductor coils are designed on an FR4 substrate and packaged using PDMS to be used as sensors in the monitoring of electrical properties of electrolytes. Experimental results on these sensors are provided and discussed. The effects of solvent, acidity of the solutions, and environmental factors on the behaviour of the sensors shall be discussed. This is followed by finite element simulations of the sensor and the effect of various parameters on the overall behaviour of the sensing device. A transformer apparatus is also constructed and experimental data are provided for it. An electrolyte is placed on one of the coils of the transformer and scattering parameters are looked upon for data analysis. The results obtained using the FE method, is then used to obtain further information about the principle of operation of the device

A Continuous and Static Water Contaminant Detection System Using RF Microwave Principles

A continuous, static, and non-interfering water contaminant detection method is presented to measure specific water contaminants (NaCl, MgCl2, and mixture of NaCl and MgCl2) using RF microwave principles. A coil is mounted on the surface of a glass tube and the liquid sample is placed inside of the tube. An external magnetic field generated by the coil continuously measures changes in radio frequency energy. The non-contact feature of the device allows a long sensor lifetime with high sensitivity for real-time measurements. The measurement parameter is reflection coefficient (S11) and the operating frequency is 10 MHz – 5 GHz. For NaCl and MgCl2, 11 different concentrations (1000 ppm – 400 ppb) liquid solutions are prepared. Amplitude changes and frequency shifts are noticeable among different materials and concentrations. Different test materials have different radio frequencies at which they undergo excitation and the responses are identified in S11 measurement. A machine learning algorithm is introduced to analyze the measured S11 data. A support vector regressor (SVR) model is trained using the measured data of various salt samples. The training data is constructed by concatenating the 20,000 amplitudes and 20,000 phase values from the measured S11 data. The hyperparameters of the SVR are optimized using 10-fold cross-validation method. Based on the trained model, the algorithm predicts the concentrations of the liquid samples. The experimental results indicate that the device can detect concentrations as low as 400 ppb with high accuracy

Monitoring microbial metabolites using an inductively coupled resonance circuit

We present a new approach to monitor microbial population dynamics in emulsion droplets via changes in metabolite composition, using an inductively coupled LC resonance circuit. The signal measured by such resonance detector provides information on the magnetic field interaction with the bacterial culture, which is complementary to the information accessible by other detection means, based on electric field interaction, i.e. capacitive or resistive, as well as optical techniques. Several charge-related factors, including pH and ammonia concentrations, were identified as possible contributors to the characteristic of resonance detector profile. The setup enables probing the ionic byproducts of microbial metabolic activity at later stages of cell growth, where conventional optical detection methods have no discriminating power

Development of biosensors for molecular analysis

Tese dout., Ciências Biotecnológicas, Universidade do Algarve, 2007This thesis deals with the application of piezoelectric sensors, namely quartz crystal microbalances (QCM), for the study of molecular interactions and for biomedical purposes. Piezoelectric transduction allows the detection of biorecognition events and its operation depends on the mechanical vibration of the crystal whose resonance frequency is sensible to surface mass changes. But in liquid medium these sensors also respond to the properties of solutions and adsorbed films. Thus, a major aspect explored in this thesis is the expansion of the physical understanding of the behavior of these sensors in liquid medium and also how organic or biological layers can affect the results and their interpretation. This was achieved using impedance analysis where the behavior of the piezoelectric sensor is interpreted with the help of equivalent electric RLC circuits. The potential electroacoustic interference of charged species in solution was particularly studied and a new equivalent circuit is proposed in order to detect and quantify these effects with the assistance of impedance analysis. The efficiency of the key steps taken for the development of our QCM system, was experimentally demonstrated with the qualitative and quantitative analysis of the process of ii 11-hydroxy-1-undecanothiol SAMs (self-assembled monolayers) formation and the recognition of streptavidin in aqueous medium using biotin modified sensors. Finally the work was focused on the development of a new detection tool for the HIV1 virus using piezoelectric immunosensors based on recombinant antibodies to detect the Vif molecule (virion infectivity factor). The developed immunosensors selectively detected Vif in aqueous solutions and presented specificity and good sensibility when detecting the target protein in complex samples like mixtures of proteins and cell extracts

Marshall Space Flight Center Research and Technology Report 2019

Today, our calling to explore is greater than ever before, and here at Marshall Space Flight Centerwe make human deep space exploration possible. A key goal for Artemis is demonstrating and perfecting capabilities on the Moon for technologies needed for humans to get to Mars. This years report features 10 of the Agencys 16 Technology Areas, and I am proud of Marshalls role in creating solutions for so many of these daunting technical challenges. Many of these projects will lead to sustainable in-space architecture for human space exploration that will allow us to travel to the Moon, on to Mars, and beyond. Others are developing new scientific instruments capable of providing an unprecedented glimpse into our universe. NASA has led the charge in space exploration for more than six decades, and through the Artemis program we will help build on our work in low Earth orbit and pave the way to the Moon and Mars. At Marshall, we leverage the skills and interest of the international community to conduct scientific research, develop and demonstrate technology, and train international crews to operate further from Earth for longer periods of time than ever before first at the lunar surface, then on to our next giant leap, human exploration of Mars. While each project in this report seeks to advance new technology and challenge conventions, it is important to recognize the diversity of activities and people supporting our mission. This report not only showcases the Centers capabilities and our partnerships, it also highlights the progress our people have achieved in the past year. These scientists, researchers and innovators are why Marshall and NASA will continue to be a leader in innovation, exploration, and discovery for years to come

Nanogap capacitive biosensor for label-free aptamer-based protein detection

Recent advances in nanotechnology offer a new platform for the label free detection of biomolecules at ultra-low concentrations. Nano biosensors are emerging as a powerful method of improving device performance whilst minimizing device size, cost and fabrication times. Nanogap capacitive biosensors are an excellent approach for detecting biomolecular interactions due to the ease of measurement, low cost equipment needed and compatibility with multiplex formats.This thesis describes research into the fabrication of a nanogap capacitive biosensor and its detection results in label-free aptamer-based protein detection for proof of concept. Over the last four decades many research groups have worked on fabrication and applications of these type of biosensors, with different approaches, but there is much scope for the improvement of sensitivity and reliability. Additionally, the potential of these sensors for use in commercial markets and in everyday life has yet to be realized.Initial work in the field was limited to high frequency (>100 kHz) measurements only, since at low frequency there is significant electronic thermal noise (=4kBTR) from the electrical double layer (EDL). This was a significant drawback since this noise masked most of the important information from biomolecular interactions of interest. A novel approach to remove this parasitic noise is to minimize the EDL impedance by reducing the capacitor electrode separation to less than the EDL thickness. In the case of aptamer functionalized electrodes, this is particularly advantageous since device sensitivity is increased as the dielectric volume is better matched to the size of the biomolecules and their binding to the electrode surface. This work has demonstrated experimentally the concepts postulated theoretically.In this work we have fabricated a large area (100 x 5 μm x 5 μm) vertically oriented capacitive nanogap biosensor with a 40 nm electrode separation between two gold electrodes. A silicon dioxide support layer separates the two electrodes and this is partially etched (approximately 800 nm from both sides of each 5 μm x 5 μm capacitor), leaving an area of the gold electrodes available for thiol-aptamer functionalization.AC impedance spectroscopy measurements were performed with the biosensor in the presence of air, D.I. water, various ionic strength buffer solutions and aptamer/protein pairs inside the nanogap. Applied frequencies were from 1Hz to 500 kHz at 20 mV AC voltage with 0 DC. We obtained relative permittivity results as a function of frequency for air (ɛ=1) and DI water (ɛ~80) which compares very favorably with previous works done by different research groups.The sensitivity and response of the sensors to buffer solution (SSC buffer) with various ionic strengths (0.1x SSC, 0.2x SSC, 0.5x SSC and 1x SSC) was studied in detail. It was found that in the low frequency region (<1 kHz) the relative permittivity (capacitance) was broadly constant, that means it is independent from the applied frequency in this range. With increasing buffer concentration, the relative permittivity starts to increase (from ɛ=170 for 0.1x SSC to ɛ=260 for 1x SSC).The sensor performance was further investigated for aptamer-based protein detection, human alpha thrombin aptamers and human alpha thrombin protein pairs were selected for proof of concept. Aptamers were functionalized into the gold electrode surface with the Self-Assembly-Monolayer (SAM) method and measurements were performed in the presence of 0.5x SSC buffer solution (ɛ=180). Then the hybridization step was carried out with 1 μM of human alpha thrombin protein followed by measurements in the presence of the same buffer (ɛ=130). The response of the sensors with different solutions inside the nanogap was studied at room temperature (5 working devices were tested for each step). The replacement of the buffer solution (ɛ=250) with lower relative permittivity biomolecules (aptamer ɛ=180) and further binding proteins to immobilized aptamer (ɛ=130) was studied. To validate these results, a control experiment was carried out using different aptamers, in this case which are not able to bind to human alpha thrombin protein. It was found that the relative permittivity did not change after the hybridization step compared to the aptamer functionalization step, which indicates that the sensors performance is highly sensitive and reliable.This work serves as a proof of concept for a novel nanogap based biosensor with the potential to be used for many applications in environmental, food industry and medical industry. The fabrication method has been shown to be reliable and consistent with the possibility of being easily commercialized for mass production for use in laboratories for the analysis of a wide range of samples