an industrial E. coli case study

The authors thank Chi Chung (Tim) Choi and Paul Rice for their collaboration in batch production and performing off-line sample analysis and Carl Hémond for providing the RP-HPLC assay and results. The authors thank Paul Rice also for critical reading of the manuscript. © Copyright 2017 Elsevier B.V., All rights reserved.Process understanding is emphasized in the process analytical technology initiative and the quality by design paradigm to be essential for manufacturing of biopharmaceutical products with consistent high quality. A typical approach to developing a process understanding is applying a combination of design of experiments with statistical data analysis. Hybrid semi-parametric modeling is investigated as an alternative method to pure statistical data analysis. The hybrid model framework provides flexibility to select model complexity based on available data and knowledge. Here, a parametric dynamic bioreactor model is integrated with a nonparametric artificial neural network that describes biomass and product formation rates as function of varied fed-batch fermentation conditions for high cell density heterologous protein production with E. coli. Our model can accurately describe biomass growth and product formation across variations in induction temperature, pH and feed rates. The model indicates that while product expression rate is a function of early induction phase conditions, it is negatively impacted as productivity increases. This could correspond with physiological changes due to cytoplasmic product accumulation. Due to the dynamic nature of the model, rational process timing decisions can be made and the impact of temporal variations in process parameters on product formation and process performance can be assessed, which is central for process understanding.publishersversionpublishe

An approach to convert vertex-based 3D representations to combinatorial B-splines for real-time visual collaboration

Scientific Visualization and Virtual Reality are increasingly being used for the design of complex systems. These technologies offer powerful capabilities to make decisions that are cost and time effective. The next logical extension is to collaborate with these visual models in real-time, where parts of a design team are geographically separated. Specifically, visual collaboration enables ideas and proposed changes to be discussed exactly on a virtual model of a product. However, high-end visualization hardware and Internet technologies impede widespread use of real-time visual collaboration due to the large amount of data from which these representations are created. These data are typically in the form of 3D vertex-based models, which offer a high degree of realism when displayed, but at a price of storage, rendering speeds and processing efficiency. The more realistic the representation desired, the larger the number of vertices required and hence the higher the file size. In this paper, we propose a new data modeling and handling technique where traditional vertex-based models are converted into combinatorial B-Spline based wire-frame models that allow realtime visual collaboration in the context of typical virtual reality systems. Using appropriate filtering methods, parametric equations are computed for each curved segment in a vertexbased representation and bundled together with sampled linear segments of the model. The computed parametric equation based models occupy only a fraction of the size when compared to the original vertex-based models. These lightweight models can easily be transmitted over the Internet, in real-time, for viewing with a platform independent visual client program. The proposed methods were tested on several example data files to prove the method’s effectiveness

Hybrid client-server and P2P network for web-based collaborative 3D design

National audienceOur proposed research project is to enable 3D distributed visualization and manipulation involving collaborative effort through the use of web-based technologies. Our project resulted from a wide collaborative application research fields: Computer Aided Design (CAD), Building Information Modeling (BIM) or Product Life Cycle Management (PLM) where design tasks are often performed in teams and need a fluent communication system. The system allows distributed remote assembling in 3D scenes with real-time updates for the users. This paper covers this feature using hybrid networking solution: a client-server architecture (REST) for 3D rendering (WebGL) and data persistence (NoSQL) associated to an automatically built peer-to-peer (P2P) mesh for real-time communication between the clients (WebRTC). The approach is demonstrated through the development of a web-platform prototype focusing on the easy manipulation, fine rendering and light update messages for all participating users. We provide an architecture and a prototype to enable users to design in 3D together in real time with the benefits of web based online collaboration

Constraint-Enabled Design Information Representation for Mechanical Products Over the Internet

Global economy has made manufacturing industry become more distributed than ever before. Product design requires more involvement from various technical disciplines at different locations. In such a geographically and temporally distributed environment, efficient and effective collaboration on design is vital to maintain product quality and organizational competency. Interoperability of design information is one of major barriers for collaborative design. Current standard CAD data formats do not support design collaboration effectively in terms of design information and knowledge capturing, exchange, and integration within the design cycle. Multidisciplinary design constraints cannot be represented and transferred among different groups, and design information cannot be integrated efficiently within a distributed environment. Uncertainty of specification cannot be modeled at early design stages, while constraints for optimization are not embedded in design data. In this work, a design information model, Universal Linkage model, is developed to represent design related information for mechanical products in a distributed form. It incorporates geometric and non-geometric constraints with traditional geometry and topology elements, thus allows more design knowledge sharing in collaborative design. Segments of design data are linked and integrated into a complete product model, thus support lean design information capturing, storage, and query. The model is represented by Directed Hyper Graph and Product Markup Language to preserve extensibility and openness. Incorporating robustness consideration, an Interval Geometric Modeling scheme is presented, in which numerical parameters are represented by interval values. This scheme is able to capture uncertainty and inexactness of design and reduces the chances of conflict in constraint imposition. It provides a unified constraint representation for the process of conceptual design, detailed design, and design optimization. Corresponding interval constraint solving methods are studied

Cloud-Based Collaborative 3D Modeling to Train Engineers for the Industry 4.0

In the present study, Autodesk Fusion 360 software (which includes the A360 environment) is used to train engineering students for the demands of the industry 4.0. Fusion 360 is a tool that unifies product lifecycle management (PLM) applications and 3D-modeling software (PDLM—product design and life management). The main objective of the research is to deepen the students’ perception of the use of a PDLM application and its dependence on three categorical variables: PLM previous knowledge, individual practices and collaborative engineering perception. Therefore, a collaborative graphic simulation of an engineering project is proposed in the engineering graphics subject at the University of La Laguna with 65 engineering undergraduate students. A scale to measure the perception of the use of PDLM is designed, applied and validated. Subsequently, descriptive analyses, contingency graphical analyses and non-parametric analysis of variance are performed. The results indicate a high overall reception of this type of experience and that it helps them understand how professionals work in collaborative environments. It is concluded that it is possible to respond to the demand of the industry needs in future engineers through training programs of collaborative 3D modeling environments

Specifications and Development of Interoperability Solution dedicated to Multiple Expertise Collaboration in a Design Framework

This paper describes the specifications of an interoperability platform based on the PPO (Product Process Organization) model developed by the French community IPPOP in the context of collaborative and innovative design. By using PPO model as a reference, this work aims to connect together heterogonous tools used by experts easing data and information exchanges. After underlining the growing needs of collaborative design process, this paper focuses on interoperability concept by describing current solutions and their limits. Then a solution based on the flexibility of the PPO model adapted to the philosophy of interoperability is proposed. To illustrate these concepts, several examples are more particularly described (robustness analysis, CAD and Product Lifecycle Management systems connections)

Adopting building information modeling (BIM) as collaboration platform in the design industry

This paper discusses the preliminary findings of an ongoing research project aimed at developing a technological, operational and strategic analysis of adopting BIM in AEC/FM (Architecture-Engineering-Construction/Facility Management) industry as a collaboration tool. Outcomes of the project will provide specifications and guidelines as well as establish industry standards for implementing BIM in practice. This research primarily focuses on BIM model servers as a collaboration platform, and hence the guidelines are aimed at enhancing collaboration capabilities. This paper reports on the findings from: (1) a critical review of latest BIM literature and commercial applications, and (2) workshops with focus groups on changing work-practice, role of technology, current perception and expectations of BIM. Layout for case studies being undertaken is presented. These findings provide a base to develop comprehensive software specifications and national guidelines for BIM with particular emphasis on BIM model servers as collaboration platforms