Sensing Cell-Culture Assays with Low-Cost Circuitry

An alternative approach for cell-culture end-point protocols is proposed herein. This new technique is suitable for real-time remote sensing. It is based on Electrical Cell-substrate Impedance Spectroscopy (ECIS) and employs the Oscillation-Based Test (OBT) method. Simple and straightforward circuit blocks form the basis of the proposed measurement system. Oscillation parameters – frequency and amplitude – constitute the outcome, directly correlated with the culture status. A user can remotely track the evolution of cell cultures in real time over the complete experiment through a web tool continuously displaying the acquired data. Experiments carried out with commercial electrodes and a well-established cell line (AA8) are described, obtaining the cell number in real time from growth assays. The electrodes have been electrically characterized along the design flow in order to predict the system performance and the sensitivity curves. Curves for 1-week cell growth are reported. The obtained experimental results validate the proposed OBT for cell-culture characterization. Furthermore, the proposed electrode model provides a good approximation for the cell number and the time evolution of the studied cultures.España, Feder TEC2013-46242-C3-1-

Design criteria for flight evaluation. Monograph 4 - Control system evaluation

Methods and analyses for flight evaluation of control systems for multistage launch vehicle

Prospect for Charge Current Neutrino Interactions Measurements at the CERN-PS

Tensions in several phenomenological models grew with experimental results on neutrino/antineutrino oscillations at Short-Baseline (SBL) and with the recent, carefully recomputed, antineutrino fluxes from nuclear reactors. At a refurbished SBL CERN-PS facility an experiment aimed to address the open issues has been proposed [1], based on the technology of imaging in ultra-pure cryogenic Liquid Argon (LAr). Motivated by this scenario a detailed study of the physics case was performed. We tackled specific physics models and we optimized the neutrino beam through a full simulation. Experimental aspects not fully covered by the LAr detection, i.e. the measurements of the lepton charge on event-by-event basis and their energy over a wide range, were also investigated. Indeed the muon leptons from Charged Current (CC) (anti-)neutrino interactions play an important role in disentangling different phenomenological scenarios provided their charge state is determined. Also, the study of muon appearance/disappearance can benefit of the large statistics of CC muon events from the primary neutrino beam. Results of our study are reported in detail in this proposal. We aim to design, construct and install two Spectrometers at "NEAR" and "FAR" sites of the SBL CERN-PS, compatible with the already proposed LAr detectors. Profiting of the large mass of the two Spectrometers their stand-alone performances have also been exploited.Comment: 70 pages, 38 figures. Proposal submitted to SPS-C, CER

A Proposal for a Three Detector Short-Baseline Neutrino Oscillation Program in the Fermilab Booster Neutrino Beam

A Short-Baseline Neutrino (SBN) physics program of three LAr-TPC detectors located along the Booster Neutrino Beam (BNB) at Fermilab is presented. This new SBN Program will deliver a rich and compelling physics opportunity, including the ability to resolve a class of experimental anomalies in neutrino physics and to perform the most sensitive search to date for sterile neutrinos at the eV mass-scale through both appearance and disappearance oscillation channels. Using data sets of 6.6e20 protons on target (P.O.T.) in the LAr1-ND and ICARUS T600 detectors plus 13.2e20 P.O.T. in the MicroBooNE detector, we estimate that a search for muon neutrino to electron neutrino appearance can be performed with ~5 sigma sensitivity for the LSND allowed (99% C.L.) parameter region. In this proposal for the SBN Program, we describe the physics analysis, the conceptual design of the LAr1-ND detector, the design and refurbishment of the T600 detector, the necessary infrastructure required to execute the program, and a possible reconfiguration of the BNB target and horn system to improve its performance for oscillation searches.Comment: 209 pages, 129 figure

MIMO First and Second Order Discrete Sliding Mode Controls of Uncertain Linear Systems under Implementation Imprecisions

The performance of a conventional model-based controller significantly depends on the accuracy of the modeled dynamics. The model of a plant's dynamics is subjected to errors in estimating the numerical values of the physical parameters, and variations over operating environment conditions and time. These errors and variations in the parameters of a model are the major sources of uncertainty within the controller structure. Digital implementation of controller software on an actual electronic control unit (ECU) introduces another layer of uncertainty at the controller inputs/outputs. The implementation uncertainties are mostly due to data sampling and quantization via the analog-to-digital conversion (ADC) unit. The failure to address the model and ADC uncertainties during the early stages of a controller design cycle results in a costly and time consuming verification and validation (V&V) process. In this paper, new formulations of the first and second order discrete sliding mode controllers (DSMC) are presented for a general class of uncertain linear systems. The knowledge of the ADC imprecisions is incorporated into the proposed DSMCs via an online ADC uncertainty prediction mechanism to improve the controller robustness characteristics. Moreover, the DSMCs are equipped with adaptation laws to remove two different types of modeling uncertainties (multiplicative and additive) from the parameters of the linear system model. The proposed adaptive DSMCs are evaluated on a DC motor speed control problem in real-time using a processor-in-the-loop (PIL) setup with an actual ECU. The results show that the proposed SISO and MIMO second order DSMCs improve the conventional SISO first order DSMC tracking performance by 69% and 84%, respectively. Moreover, the proposed adaptation mechanism is able to remove the uncertainties in the model by up to 90%.Comment: 10 pages, 11 figures, ASME 2017 Dynamic Systems and Control Conferenc

Performances of conformal and planar arrays

Static and dynamic deformations can have a severe impact on the performance of conformal antennas on aircrafts and other vehicles. Therefore it is essential to study the different deformation and vibration mechanisms and their influence on the antenna's radiation pattern. This presentation gives an overview of different approaches concerning electromagnetic modelling of array antennas and investigations on antenna deformations presented in the scope of TG20