Comparative study of Sustainability Metrics for Face Milling AISI 1045 in different Machining Centers

Comunicación presentada a MESIC 2019 8th Manufacturing Engineering Society International Conference (Madrid, 19-21 de Junio de 2019)The objective of this study is to compare a set of sustainability metrics between different manufacturing resources applied to high performances machining centers. The research compares distributed scenarios in order to find the optimal conditions that allow the minimum consumed power and the minimum roughness when performing face milling operations of AISI 1045 steel. The set of experiments for the surface machining was carried out considering different path strategies in three main directions for two dimensional movements of the tool. The selected experiments considered the main axis movement, the perpendicular axis movement and a 45 degrees movement. Besides, it was considered the feed rate speed and the cutting depth. The design of experiments was developed with the Taguchi method considering an orthogonal matrix of L27 design type, and three levels of experimental design, and the analysis of variance and noise signal were performed. The methodology to determine the lowest power consumed and the best surface quality allowed to establish the working condition in the most sustainable machining. The results show how the cutting parameters influence in each manufacturing resource

Ro 15-4513 Antagonizes Alcohol-Induced Sedation in Mice Through aß¿2-type GABA(A) Receptors

Peer reviewedPublisher PD

A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells.

Elevated glucose consumption is fundamental to cancer, but selectively targeting this pathway is challenging. We develop a high-throughput assay for measuring glucose consumption and use it to screen non-small-cell lung cancer cell lines against bioactive small molecules. We identify Milciclib that blocks glucose consumption in H460 and H1975, but not in HCC827 or A549 cells, by decreasing SLC2A1 (GLUT1) mRNA and protein levels and by inhibiting glucose transport. Milciclib blocks glucose consumption by targeting cyclin-dependent kinase 7 (CDK7) similar to other CDK7 inhibitors including THZ1 and LDC4297. Enhanced PIK3CA signaling leads to CDK7 phosphorylation, which promotes RNA Polymerase II phosphorylation and transcription. Milciclib, THZ1, and LDC4297 lead to a reduction in RNA Polymerase II phosphorylation on the SLC2A1 promoter. These data indicate that our high-throughput assay can identify compounds that regulate glucose consumption and that CDK7 is a key regulator of glucose consumption in cells with an activated PI3K pathway