105,886 research outputs found
Sources and transformations of dissolved lignin phenols and chromophoric dissolved organic matter in Otsuchi Bay, Japan
Dissolved lignin phenols and optical properties of dissolved organic matter (DOM) were measured to investigate the sources and transformations of terrigenous DOM (tDOM) in Otsuchi Bay, Japan. Three rivers discharge into the bay, and relatively high values of syringyl:vanillyl phenols (0.73 Âą 0.07) and cinnamyl:vanillyl phenols (0.33 Âą 0.10) indicated large contributions of non-woody angiosperm tissues to lignin and tDOM. The physical mixing of river and seawater played an important role in controlling the concentrations and distributions of lignin phenols and chromophoric DOM (CDOM) optical properties in the bay. Lignin phenol concentrations and the CDOM absorption coefficient at 350 nm, a(350), were strongly correlated in river and bay waters. Measurements of lignin phenols and CDOM in bay waters indicated a variety of photochemical and biological transformations of tDOM, including oxidation reactions, photobleaching and a decrease in molecular weight. Photodegradation and biodegradation of lignin and CDOM were investigated in decomposition experiments with river water and native microbial assemblages exposed to natural sunlight or kept in the dark. There was a rapid and substantial removal of lignin phenols and CDOM during the first few days in the light treatment, indicating transformations of tDOM and CDOM can occur soon after discharge of buoyant river water into the bay. The removal of lignin phenols was slightly greater in the dark (34%) than in the light (30%) during the remaining 59 days of the incubation. Comparison of the light and dark treatments indicated biodegradation was responsible for 67% of total lignin phenols removal during the 62-day incubation exposed to natural sunlight, indicating biodegradation is a dominant removal process in Otsuchi Bay.Ministry of Education, Culture, Sports, Science, and Technology; 1504137 - National Science Foundatio
Synthesis of 2-Acylphenol and Flavene Derivatives from the Ruthenium-Catalyzed Oxidative C-H Acylation of Phenols with Aldehydes
The cationic ruthenium hydride complex [(C6H6)(PCy3)(CO)RuH]+BF4â has been found to be an effective catalyst for the oxidative CâH coupling reaction of phenols with aldehydes to give 2-acylphenol compounds. The coupling of phenols with Îą,β-unsaturated aldehydes selectively gives the flavene derivatives. The catalytic method mediates direct oxidative CâH coupling of phenol and aldehyde substrates without using any metal oxidants or forming wasteful byproducts
Redox and Spectroscopic Properties of Iron Porphyrin Nitroxyl in the Presence of Weak Acids
The spectroelectrochemistry and voltammetry of Fe(OEP) (NO) in the presence of substituted phenols was studied. Cyclic voltammetry showed that two closely spaced waves were observed for the reduction of Fe(OEP) (NO) in the presence of substituted phenols. The first wave was a single electron reduction under voltammetric conditions. The second wave was kinetically controlled, multielectron process. Visible spectroelectrochemistry of Fe(OEP) (NO) in the presence of substituted phenols showed that three species were present during the electrolysis. Additional spectroscopic studies indicated that the two reduction species were Fe(OEP) (HNO) and Fe(OEP)(H2NOH). The Fe(OEP) (HNO) species, which can be generated chemically, was stable over a period of hours. Additional acid did not lead to further protonation. Proton NMR spectroscopy confirmed the Fe(OEP) (HNO) species could be deprotonated under basic conditions. The third species, Fe(OEP)(H2NOH), was generated by the further reduction of the chemically generated Fe(OEP) (HNO) complex. Both the Fe(OEP) (HNO) and Fe(OEP)(H2NOH) complexes could be slowly oxidized back to Fe(OEP) (NO). At millimolar concentrations of Fe(OEP) (HNO), there was no evidence for the disproportionation of Fe(OEP) (HNO) to Fe(OEP) (NO) and H2 in the presence of substituted phenols. Nor was there evidence for the generation of N2O. The FTIR spectroelectrochemistry showed changes in the infrared spectra in the presence of substituted phenols, but no isotopic sensitive bands were observed for the reduced species between 1450 and 1200 cmâ1. This may be because the νNO band shifted into a region (1500â1450 cmâ1) where it would be difficult to observe
Assessment of the genotoxicity of olive mill waste water (OMWW) with the Vicia faba micronucleus test
The present study concerns the genotoxicity of olive mill waste water (OMWW) generated in mills producing olive oil in Morocco. The Vicia faba micronucleus test was used to evaluate the genotoxicity of OMWW and the six major phenolic compounds identified by HPLC in this effluent. Five dilutions of OMWW were tested: 0.1, 1, 5, 10 and 20%. Maleic hydrazide was used as a positive control. The results showed that OMWW was genotoxic at 10% dilution. In order to investigate the components involved in this genotoxicity, the six major phenols present in this effluent, oleuropein, gallic acid, 4-hydroxyphenyl acetic acid, caffeic acid, paracoumaric acid and veratric acid, were studied at concentrations corresponding to the genotoxic concentration of the OMWW itself. Two phenols, gallic acid and oleuropein induced a significant increase in micronucleus frequency in Vicia faba; the four other phenols had no significant genotoxic effect. These results suggest that under the experimental conditions of our assay, OMWW genotoxicity was associated with gallic acid and oleuropein
Influence of Processing on Bioactive Substances Content and Antioxidant Properties of Apple PurĂŠe from Organic and Conventional Production in Poland
The organic food market is developing dynamically in many European countries and therefore studies concerning the nutritive value of organically produced foods are becoming increasingly important. It was found appropriate to conduct studies on selected bioactive substances and antioxidant properties of apple preserves prepared from organic vs. conventional apples. Three apple cultivars, Lobo, Boskoop and Cortland, were grown in organic and conventional orchards in the Mazovia region in Poland. Dry matter, total phenols, vitamin C, total flavones and antioxidant activity were determined in fresh and pasteurized apple purĂŠe. The apple purĂŠe prepared from the organic apples contained significantly more total phenols, vitamin C, total flavones and showed a higher antioxidant capacity than the preserves prepared from conventional apples. Processing had a negative effect on both antioxidant capacity and bioactive substances. After pasteurization, the content of vitamin C, total phenols and flavones and antioxidant properties have decreased in the apple purĂŠe from both agricultural systems (organic and conventional)
Identification of plasma and urinary metabolites and catabolites derived from orange juice (poly)phenols: analysis by high-performance liquid chromatographyâhigh-resolution mass spectrometry
Orange juice is a rich source of (poly)phenols, in particular, the flavanones hesperetin-7-O-rutinoside and naringenin-7-O-rutinoside. Following the acute consumption of 500 mL of orange juice containing 398 Îźmol of (poly)phenols by 12 volunteers, 0â24 h plasma and urine samples were analyzed by targeted high-performance liquid chromatographyâhigh-resolution mass spectrometry in order to identify flavanone metabolites and phenolic acid and aromatic catabolites. A total of 19 flavanone metabolitesâcomprising di-O-glucuronide, O-glucuronide, O-glucuronyl-sulfate, and sulfate derivatives of hesperetin, naringenin, and eriodictyolâand 65 microbial-derived phenolic catabolites, such as phenylpropanoid, phenylpropionic, phenylacetic, benzoic, and hydroxycarboxylic acids and benzenetriol and benzoylglycine derivatives, including free phenolics and phase II sulfate, glucuronide, and methyl metabolites, were identified or partially identified in plasma and/or urine samples. The data obtained provide a detailed evaluation of the fate of orange juice (poly)phenols as they pass through the gastrointestinal tract and are absorbed into the circulatory system prior to renal excretion. Potential pathways for these conversions are proposed
Catalytic molecularly imprinted polymer membranes: Development of the biomimetic sensor for phenols detection
Portable biomimetic sensor devices for the express control of phenols content in
water were developed. The synthetic binding sites mimicking active site of the
enzyme tyrosinase were formed in the structure of free-standing molecularly
imprinted polymer membranes. Molecularly imprinted polymer membranes with the
catalytic activity were obtained by co-polymerization of the complex Cu
(II)âcatecholâurocanic acid ethyl ester with (tri)ethyleneglycoldimethacrylate,
and oligourethaneacrylate. Addition of the elastic component
oligourethaneacrylate provided formation of the highly cross-linked polymer with
the catalytic activity in a form of thin, flexible, and mechanically stable
membrane. High accessibility of the artificial catalytic sites for the
interaction with the analyzed phenol molecules was achieved due to addition of
linear polymer (polyethyleneglycol Mw 20,000) to the initial monomer mixture
before the polymerization. As a result, typical semi-interpenetrating polymer
networks (semi-IPNs) were formed. The cross-linked component of the semi-IPN was
represented by the highly cross-linked catalytic molecularly imprinted polymer,
while the linear one was represented by polyethyleneglycol Mw 20,000. Extraction
of the linear polymer from the fully formed semi-IPN resulted in formation of
large pores in the membranesâ structure. Concentration of phenols in the
analyzed samples was detected using universal portable device oxymeter with the
oxygen electrode in a close contact with the catalytic molecularly imprinted
polymer membrane as a transducer. The detection limit of phenols detection using
the developed sensor system based on polymersâbiomimics with the optimized
composition comprised 0.063 mM, while the linear range of the sensor comprised
0.063â1 mM. The working characteristics of the portable sensor devices were
investigated. Storage stability of sensor systems at room temperature comprised
12 months (87%). As compared to traditional methods of phenols detection the
developed sensor system is characterized by simplicity of operation,
compactness, an
Endogenous levels of reducing sugars, free amino acids and phenols during various stages of in vitro culture of cotton (Gossypium Spp.)
Somatic embryogenesis is widely preferred as the regeneration route for in vitro studies in cotton.
However, the regeneration efficiency through this approach is low; a problem that is believed to be as a result
of the biochemical properties of the plant. The objective of this study was to investigate possible relationships
between three biochemical factors (reducing sugars, phenols, and free amino acids) and somatic
embryogenesis. In vitro cultures of the different embryogenic and non-embryogenic cultivals were established.
The levels of reducing sugars, phenols and free amino acids were determined at different developmental stages
of the cultures. Higher levels of reducing sugars and lower level of phenol were observed in embryogenic
cultivars compared to their non-embryogenic counterparts. There was a general increase in the levels of free
amino acids, which decreased with time in the highly embryogenic cultivars, whereas the levels remained high
in the poorly embryogenic and non-embryogenic cultivars. The higher content of phenols and free amino acids
may be implicated in the poor somatic embryogenic response. The data show that there are factors that may
serve as markers of somatic embryogenesis in cotton, which need to be empirically determined for any
particular cultivar chosen for genetic improvement through embryogenesis
Effects of gabergic phenols on the dynamic and structure of lipid bilayers: A molecular dynamic simulation approach
Îł-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the vertebrate and invertebrate nervous system. GABAA receptors are activated by GABA and their agonists, and modulated by a wide variety of recognized drugs, including barbiturates, anesthetics, and benzodiazepines. The phenols propofol, thymol, chlorothymol, carvacrol and eugenol act as positive allosteric modulators on GABAA-R receptor. These GABAergic phenols interact with the lipid membrane, therefore, their anesthetic activity could be the combined result of their specific activity (with receptor proteins) as well as nonspecific interactions (with surrounding lipid molecules) modulating the supramolecular organization of the receptor environment. Therefore, we aimed to contribute to a description of the molecular events that occur at the membrane level as part of the mechanism of general anesthesia, using a molecular dynamic simulation approach. Equilibrium molecular dynamics simulations indicate that the presence of GABAergic phenols in a DPPC bilayer orders lipid acyl chains for carbons near the interface and their effect is not significant at the bilayer center. Phenols interacts with the polar interface of phospholipid bilayer, particularly forming hydrogen bonds with the glycerol and phosphate group. Also, potential of mean force calculations using umbrella sampling show that propofol partition is mainly enthalpic driven at the polar region and entropic driven at the hydrocarbon chains. Finally, potential of mean force indicates that propofol partition into a gel DPPC phase is not favorable. Our in silico results were positively contrasted with previous experimental data.Fil: Miguel, Virginia. Consejo Nacional de Investigaciones CientĂficas y TĂŠcnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones BiolĂłgicas y TecnolĂłgicas. Universidad Nacional de CĂłrdoba. Facultad de Ciencias Exactas, FĂsicas y Naturales. Instituto de Investigaciones BiolĂłgicas y TecnolĂłgicas; ArgentinaFil: Villarreal, Marcos Ariel. Consejo Nacional de Investigaciones CientĂficas y TĂŠcnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones en FĂsico-quĂmica de CĂłrdoba. Universidad Nacional de CĂłrdoba. Facultad de Ciencias QuĂmicas. Instituto de Investigaciones en FĂsico-quĂmica de CĂłrdoba; ArgentinaFil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones CientĂficas y TĂŠcnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones BiolĂłgicas y TecnolĂłgicas. Universidad Nacional de CĂłrdoba. Facultad de Ciencias Exactas, FĂsicas y Naturales. Instituto de Investigaciones BiolĂłgicas y TecnolĂłgicas; Argentin
- âŚ