9,232 research outputs found

    Face valid phenotypes in a mouse model of the most common mutation in EEF1A2 related neurodevelopmental disorder, E122K

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    De novo heterozygous missense mutations in EEF1A2, encoding neuromuscular translation-elongation factor eEF1A2, are associated with developmental and epileptic encephalopathies. We used CRISPR/Cas9 to recapitulate the most common mutation, E122K, in mice. Although E122K heterozygotes were not observed to have convulsive seizures, they exhibited frequent electrographic seizures and EEG abnormalities, transient early motor deficits and growth defects. Both E122K homozygotes and Eef1a2-null mice developed progressive motor abnormalities, with E122K homozygotes reaching humane endpoints by P31. The null phenotype is driven by progressive spinal neurodegeneration; however, no signs of neurodegeneration were observed in E122K homozygotes. The E122K protein was relatively stable in neurons yet highly unstable in skeletal myocytes, suggesting that the E122K/E122K phenotype is instead driven by loss of function in muscle. Nevertheless, motor abnormalities emerged far earlier in E122K homozygotes than in nulls, suggesting a toxic gain of function and/or a possible dominant-negative effect. This mouse model represents the first animal model of an EEF1A2 missense mutation with face-valid phenotypes and has provided mechanistic insights needed to inform rational treatment design.</p

    Design and Evaluation of a Smooth-Locking-Based Customizable Prosthetic Knee Joint

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    Limb loss affects many people from a variety of backgrounds around the world. The most advanced commercially available prostheses for transfemoral amputees are fully active (powered) designs but remain very expensive and unavailable in the developing world. Consequently, improvements of low-cost, passive prostheses have been made to provide high-quality rehabilitation to amputees of any background. This study explores the design and evaluation of a smooth-locking-based bionic knee joint to replicate the swing phase of the human gait cycle. The two-part design was based on the condyle geometry of the interface between the femur and tibia obtained from magnetic resonance (MR) images of the human subject, while springs were used to replace the anterior and posterior cruciate ligaments. A flexible four-bar linkage mechanism was successfully achieved to provide not only rotation along a variable instantaneous axis but also slight translation in the sagittal plane, similar to the anatomical knee. We systematically evaluated the effects of different spring configurations in terms of stiffness, position, and relaxion length on knee flexion angles during walking. A good replication of the swing phase was achieved by relatively high stiffness and increased relaxation length of springs. The stance phase of the gait cycle was improved compared to some models but remained relatively flat, where further verification should be conducted. In addition, 3D printing technique provides a convenient design and manufacturing process, making the prosthesis customizable for different individuals based on subject-specific modeling of the amputee’s knee

    Review of Computational Fluid Dynamics Analysis in Biomimetic Applications for Underwater Vehicles

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    Biomimetics, which draws inspiration from nature, has emerged as a key approach in the development of underwater vehicles. The integration of this approach with computational fluid dynamics (CFD) has further propelled research in this field. CFD, as an effective tool for dynamic analysis, contributes significantly to understanding and resolving complex fluid dynamic problems in underwater vehicles. Biomimetics seeks to harness innovative inspiration from the biological world. Through the imitation of the structure, behavior, and functions of organisms, biomimetics enables the creation of efficient and unique designs. These designs are aimed at enhancing the speed, reliability, and maneuverability of underwater vehicles, as well as reducing drag and noise. CFD technology, which is capable of precisely predicting and simulating fluid flow behaviors, plays a crucial role in optimizing the structural design of underwater vehicles, thereby significantly enhancing their hydrodynamic and kinematic performances. Combining biomimetics and CFD technology introduces a novel approach to underwater vehicle design and unveils broad prospects for research in natural science and engineering applications. Consequently, this paper aims to review the application of CFD technology in the biomimicry of underwater vehicles, with a primary focus on biomimetic propulsion, biomimetic drag reduction, and biomimetic noise reduction. Additionally, it explores the challenges faced in this field and anticipates future advancements

    Southern Adventist University Undergraduate Catalog 2023-2024

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    Southern Adventist University\u27s undergraduate catalog for the academic year 2023-2024.https://knowledge.e.southern.edu/undergrad_catalog/1123/thumbnail.jp

    Biomechanics in anthropology

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    Biomechanics is the set of tools that explain organismal movement and mechanical behavior and links the organism to the physicality of the world. As such, biomechanics can relate behaviors and culture to the physicality of the organism. Scale is critical to biomechanical analyses, as the constitutive equations that matter differ depending on the scale of the question. Within anthropology, biomechanics has had a wide range of applications, from understanding how we and other primates evolved to understanding the effects of technologies, such as the atlatl, and the relationship between identity, society, culture, and medical interventions, such as prosthetics. Like any other model, there is great utility in biomechanical models, but models should be used primarily for hypothesis testing and not data generation except in the rare case where models can be robustly validated. The application of biomechanics within anthropology has been extensive, and holds great potential for the future

    3D COACHING: SPORTS BIOMECHANICAL ANALYSIS OF COLLEGIATE ATHLETICS (TRACK & FIELD)

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    Athletic (Track & Field) championships have showcased globally the great strength, power, and speed of athletes in a myriad of disciplines. Notably over the last 30 years, steady improvements in championship performances have intrigued the Athletics community—athletes, coaches, spectators—sparking interest to look further into how this caliber of athletes perform and what the training demands are to continue the pace of progress. Coaches, by nature, focus on what is familiar to them until the next ‘phenomenon’ in development and training becomes recognizable. In consequence, sports science research sources are perceived with complexity, and unused or misused by the Athletic community. Efforts led by leading sports scientists have been made in the live capture of world-class competitors during world championships to better understand, discuss, and use science within the current state of Athletics in published biomechanical reports. Although athletes have a critical role in whether achievements are met, coaching efforts are to serve the athlete's needs within the demands of each discipline. Balancing what an athlete can do biomechanically and the mechanism within a discipline is the challenge. Coaches often turn to the experiences that have built their coaching philosophy for guidance on the best approaches. With a focus on the NCAA collegiate championship, this project served as a biomechanical-driven evidence-based collection to better understand championship performance. The results justified achieving season-best sprint times and jump marks for higher seeding purposes. Furthermore, results underscored the high individuality in step characteristics during the development of acceleration and velocity of sprinters and jumpers. NCAA championships feature arguably the best collegiate and world-class competitors in Athletics. When the coaching and scientific views are taken into consideration at this level, an improved attempt at defining and appropriately applying mechanical principles to the technique and skills used can be established. Assessing kinematic parameters captured during these championships provides insight into biomechanical contributions in performances for coaches to evaluate and improve training design that will shape an athlete’s performance. An opportunity is available to add to the sports science narratives on the mechanics of Athletic disciplines using a biomechanics lens to magnify the coaches’ eye

    Understanding local neuromuscular mechanisms that explain the efficacy of interventions for patellofemoral pain

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    Patellofemoral pain (PFP) is a common and persistent knee pain complaint among all age ranges, especially highly active people. Multiple approaches have been used to understand symptom persistence, including identifying a mechanism explaining intervention benefits (i.e. changes in specific deficits in groups that show symptoms’ improvement). Research has been conducted to identify the characteristics associated with PFP, but uncertainty regarding local neuromuscular characteristics remain evident. The thesis aimed to a) identify the local neuromuscular characteristics associated with PFP, b) develop an evidence informed laboratory protocol to detect those characteristics, c) establish protocol reliability and feasibility, and d) identify interventions that can target these neuromuscular characteristics. A systematic review with meta-analysis was completed to identify the neuromuscular characteristics of all muscles that cross the knee in people with PFP compared to uninjured groups. Ten deficits within three neuromuscular domains were found. Within the electromyography (EMG) domain, a delay in Vastus medialis (VM) relative to Vastus lateralis (VL) excitation onset, a high Biceps femoris (BF) mean excitation amplitude, and a lower Hoffman-reflex amplitude of VM were identified. Within the muscle performance domain, lower isometric, concentric, and eccentric extensors peak torque and total work, lower concentric flexors peak torque, and lower rate of torque development (RTD) to reach 30%, 60% and 90% of extensors peak torque were identified. Hamstring tightness was identified within muscle flexibility domain. The systematic review was published and the results used to inform testing protocol development. A second systematic review with meta-analysis was conducted to identify interventions that can target the local deficits associated with PFP. The results indicate that currently an intervention that effectively modifies EMG characteristics cannot be identified. Predominantly, exercise interventions have effects on strength and flexibility in PFP. Specifically, hip and knee targeted exercises are found to have a potential mechanism of benefit through both characteristics categories. A unique approach was introduced within the thesis to develop a deficit-detection protocol based on systematic review results. This approach provided a comprehensive analysis of the protocols from the studies that were included in the meta-analysis. A battery of tests was developed and included; a) VM-VL excitation onset timing in step-up task, b) BF mean excitation amplitude in single-leg triple-hop test, c) isometric, d) concentric and e) eccentric extensors peak torque, f) RTD to 30%, 60% and 90% of isometric peak torque, and hamstrings flexibility. Reliability testing of the deficit-detection protocol was conducted with both uninjured and participants with PFP over two phases. Phase one evaluated the original protocols adapted from the review. Phase two was performed on the EMG and RTD domains to explore the effects of signal processing parameters on reliability, such as; onset detection thresholds modification, unnormalised signals, and the addition of absolute RTD. For the PFP group: reliable results were demonstrated for concentric and eccentric extensors peak torque; RTD of the quadriceps at 25ms, 50ms and 90% of peak torque; and hamstrings flexibility. The uninjured group showed reliable results in: unnormalised BF mean excitation amplitude; all three peak torque tests; RTD to 30% of peak torque and at 150 and 175 milliseconds; and hamstrings flexibility. To establish participant recruitment rate and retention, in addition to the acceptability of the test protocol, a preliminary feasibility study of the deficit-detection protocol was conducted. A sample of 14 participants with PFP were recruited and tested at the Mile-end campus of QMUL before and after a six weeks period. Feasibility results indicate that 25.5% were willing to participate following an online screening process (n=17/55) and 82% met the eligibility criteria following face-to-face assessment (n=14/17). Recruitment rate was 0.5 participants per week and drop-out rate was 35.2% (n=11/17). The results indicate that the protocol did not meet all a-priori feasibility criteria, but the results can inform future research planning. The thesis has successfully identified local deficits associated with PFP, developed a test protocol that demonstrates reliability in evaluating these deficits and assessed the feasibility of the protocol in individuals with PFP. Interventions to cause change within these local deficits have been identified, with gap maps demonstrating where further research is required to better align the mechanisms of treatment effects with specific deficits associated with PFP

    Determining Objective Parameters to Assess Gait Quality in Franches-Montagnes Horses for Ground Coverage and Over-Tracking - Part 2: At Trot

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    In gait quality assessments of horses, stride length (SL) is visually associated with spectacular movements of the front limbs, and described as ground coverage, while the movement of the hind limb under the body is supposedly essential to a longer over-tracking distance (OTD). To identify movement patterns with strong associations to SL and OTD, limb and body kinematics of 24 Franches-Montagnes (FM) stallions were measured with 3D optical motion capture (OMC) on a treadmill during an incremental speed test at trot (3.3–6.5 m/s). These measurements were correlated to the scores of ground coverage and over-tracking from six breeding experts. The amount of explained variance of parameters on SL and OTD were estimated using linear mixed-effect models in two models: a full model with all parameters measurable with OMC, and a reduced model with a subset of parameters measurable with inertial measurement units (IMUs). The front limb stance duration (16%) and OTD (7%) measured with OMC, or the OMC parameters front limb stance duration (24%) and suspension duration (14%) measurable with IMUs explained most variance in SL. However, four of six breeding experts were also significantly correlated (r>|0.41|) to front limb protraction angle. OTD variance was explained with OMC parameters suspension duration (10%) and hind limb contralateral pro-retraction angles (9%) or IMU-measurable parameters suspension duration (20%) and maximal pelvis pitch (5%). Four experts’ scores for over-tracking were correlated to suspension duration. These results underscore the need for precise definitions of gait quality traits

    Running to Your Own Beat:An Embodied Approach to Auditory Display Design

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    Personal fitness trackers represent a multi-billion-dollar industry, predicated on devices for assisting users in achieving their health goals. However, most current products only offer activity tracking and measurement of performance metrics, which do not ultimately address the need for technique related assistive feedback in a cost-effective way. Addressing this gap in the design space for assistive run training interfaces is also crucial in combating the negative effects of Forward Head Position, a condition resulting from mobile device use, with a rapid growth of incidence in the population. As such, Auditory Displays (AD) offer an innovative set of tools for creating such a device for runners. ADs present the opportunity to design interfaces which allow natural unencumbered motion, detached from the mobile or smartwatch screen, thus making them ideal for providing real-time assistive feedback for correcting head posture during running. However, issues with AD design have centred around overall usability and user-experience, therefore, in this thesis an ecological and embodied approach to AD design is presented as a vehicle for designing an assistive auditory interface for runners, which integrates seamlessly into their everyday environments

    Une méthode de mesure du mouvement humain pour la programmation par démonstration

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    Programming by demonstration (PbD) is an intuitive approach to impart a task to a robot from one or several demonstrations by the human teacher. The acquisition of the demonstrations involves the solution of the correspondence problem when the teacher and the learner differ in sensing and actuation. Kinesthetic guidance is widely used to perform demonstrations. With such a method, the robot is manipulated by the teacher and the demonstrations are recorded by the robot's encoders. In this way, the correspondence problem is trivial but the teacher dexterity is afflicted which may impact the PbD process. Methods that are more practical for the teacher usually require the identification of some mappings to solve the correspondence problem. The demonstration acquisition method is based on a compromise between the difficulty of identifying these mappings, the level of accuracy of the recorded elements and the user-friendliness and convenience for the teacher. This thesis proposes an inertial human motion tracking method based on inertial measurement units (IMUs) for PbD for pick-and-place tasks. Compared to kinesthetic guidance, IMUs are convenient and easy to use but can present a limited accuracy. Their potential for PbD applications is investigated. To estimate the trajectory of the teacher's hand, 3 IMUs are placed on her/his arm segments (arm, forearm and hand) to estimate their orientations. A specific method is proposed to partially compensate the well-known drift of the sensor orientation estimation around the gravity direction by exploiting the particular configuration of the demonstration. This method, called heading reset, is based on the assumption that the sensor passes through its original heading with stationary phases several times during the demonstration. The heading reset is implemented in an integration and vector observation algorithm. Several experiments illustrate the advantages of this heading reset. A comprehensive inertial human hand motion tracking (IHMT) method for PbD is then developed. It includes an initialization procedure to estimate the orientation of each sensor with respect to the human arm segment and the initial orientation of the sensor with respect to the teacher attached frame. The procedure involves a rotation and a static position of the extended arm. The measurement system is thus robust with respect to the positioning of the sensors on the segments. A procedure for estimating the position of the human teacher relative to the robot and a calibration procedure for the parameters of the method are also proposed. At the end, the error of the human hand trajectory is measured experimentally and is found in an interval between 28.528.5 mm and 61.861.8 mm. The mappings to solve the correspondence problem are identified. Unfortunately, the observed level of accuracy of this IHMT method is not sufficient for a PbD process. In order to reach the necessary level of accuracy, a method is proposed to correct the hand trajectory obtained by IHMT using vision data. A vision system presents a certain complementarity with inertial sensors. For the sake of simplicity and robustness, the vision system only tracks the objects but not the teacher. The correction is based on so-called Positions Of Interest (POIs) and involves 3 steps: the identification of the POIs in the inertial and vision data, the pairing of the hand POIs to objects POIs that correspond to the same action in the task, and finally, the correction of the hand trajectory based on the pairs of POIs. The complete method for demonstration acquisition is experimentally evaluated in a full PbD process. This experiment reveals the advantages of the proposed method over kinesthesy in the context of this work.La programmation par démonstration est une approche intuitive permettant de transmettre une tâche à un robot à partir d'une ou plusieurs démonstrations faites par un enseignant humain. L'acquisition des démonstrations nécessite cependant la résolution d'un problème de correspondance quand les systèmes sensitifs et moteurs de l'enseignant et de l'apprenant diffèrent. De nombreux travaux utilisent des démonstrations faites par kinesthésie, i.e., l'enseignant manipule directement le robot pour lui faire faire la tâche. Ce dernier enregistre ses mouvements grâce à ses propres encodeurs. De cette façon, le problème de correspondance est trivial. Lors de telles démonstrations, la dextérité de l'enseignant peut être altérée et impacter tout le processus de programmation par démonstration. Les méthodes d'acquisition de démonstration moins invalidantes pour l'enseignant nécessitent souvent des procédures spécifiques pour résoudre le problème de correspondance. Ainsi l'acquisition des démonstrations se base sur un compromis entre complexité de ces procédures, le niveau de précision des éléments enregistrés et la commodité pour l'enseignant. Cette thèse propose ainsi une méthode de mesure du mouvement humain par capteurs inertiels pour la programmation par démonstration de tâches de ``pick-and-place''. Les capteurs inertiels sont en effet pratiques et faciles à utiliser, mais sont d'une précision limitée. Nous étudions leur potentiel pour la programmation par démonstration. Pour estimer la trajectoire de la main de l'enseignant, des capteurs inertiels sont placés sur son bras, son avant-bras et sa main afin d'estimer leurs orientations. Une méthode est proposée afin de compenser partiellement la dérive de l'estimation de l'orientation des capteurs autour de la direction de la gravité. Cette méthode, appelée ``heading reset'', est basée sur l'hypothèse que le capteur passe plusieurs fois par son azimut initial avec des phases stationnaires lors d'une démonstration. Cette méthode est implémentée dans un algorithme d'intégration et d'observation de vecteur. Des expériences illustrent les avantages du ``heading reset''. Cette thèse développe ensuite une méthode complète de mesure des mouvements de la main humaine par capteurs inertiels (IHMT). Elle comprend une première procédure d'initialisation pour estimer l'orientation des capteurs par rapport aux segments du bras humain ainsi que l'orientation initiale des capteurs par rapport au repère de référence de l'humain. Cette procédure, consistant en une rotation et une position statique du bras tendu, est robuste au positionnement des capteurs. Une seconde procédure est proposée pour estimer la position de l'humain par rapport au robot et pour calibrer les paramètres de la méthode. Finalement, l'erreur moyenne sur la trajectoire de la main humaine est mesurée expérimentalement entre 28.5 mm et 61.8 mm, ce qui n'est cependant pas suffisant pour la programmation par démonstration. Afin d'atteindre le niveau de précision nécessaire, une nouvelle méthode est développée afin de corriger la trajectoire de la main par IHMT à partir de données issues d'un système de vision, complémentaire des capteurs inertiels. Pour maintenir une certaine simplicité et robustesse, le système de vision ne suit que les objets et pas l'enseignant. La méthode de correction, basée sur des ``Positions Of Interest (POIs)'', est constituée de 3 étapes: l'identification des POIs dans les données issues des capteurs inertiels et du système de vision, puis l'association de POIs liées à la main et de POIs liées aux objets correspondant à la même action, et enfin, la correction de la trajectoire de la main à partir des paires de POIs. Finalement, la méthode IHMT corrigée est expérimentalement évaluée dans un processus complet de programmation par démonstration. Cette expérience montre l'avantage de la méthode proposée sur la kinesthésie dans le contexte de ce travail
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