A Robust and Universal Metaproteomics Workflow for Research Studies and Routine Diagnostics Within 24 h Using Phenol Extraction, FASP Digest, and the MetaProteomeAnalyzer

The investigation of microbial proteins by mass spectrometry (metaproteomics) is a key technology for simultaneously assessing the taxonomic composition and the functionality of microbial communities in medical, environmental, and biotechnological applications. We present an improved metaproteomics workflow using an updated sample preparation and a new version of the MetaProteomeAnalyzer software for data analysis. High resolution by multidimensional separation (GeLC, MudPIT) was sacrificed to aim at fast analysis of a broad range of different samples in less than 24 h. The improved workflow generated at least two times as many protein identifications than our previous workflow, and a drastic increase of taxonomic and functional annotations. Improvements of all aspects of the workflow, particularly the speed, are first steps toward potential routine clinical diagnostics (i.e., fecal samples) and analysis of technical and environmental samples. The MetaProteomeAnalyzer is provided to the scientific community as a central remote server solution at www.mpa.ovgu.de.Peer Reviewe

End-Site Routing Support for IPv6 Multihoming

Multihoming is currently widely used to provide fault tolerance and traffic engineering capabilities. It is expected that, as telecommunication costs decrease, its adoption will become more and more prevalent. Current multihoming support is not designed to scale up to the expected number of multihomed sites, so alternative solutions are required, especially for IPv6. In order to preserve interdomain routing scalability, the new multihoming solution has to be compatible with Provider Aggregatable addressing. However, such addressing scheme imposes the configuration of multiple prefixes in multihomed sites, which in turn causes several operational difficulties within those sites that may even result in communication failures when all the ISPs are working properly. In this paper we propose the adoption of Source Address Dependent routing within the multihomed site to overcome the identified difficulties.Publicad

Transparent and scalable client-side server selection using netlets

Replication of web content in the Internet has been found to improve service response time, performance and reliability offered by web services. When working with such distributed server systems, the location of servers with respect to client nodes is found to affect service response time perceived by clients in addition to server load conditions. This is due to the characteristics of the network path segments through which client requests get routed. Hence, a number of researchers have advocated making server selection decisions at the client-side of the network. In this paper, we present a transparent approach for client-side server selection in the Internet using Netlet services. Netlets are autonomous, nomadic mobile software components which persist and roam in the network independently, providing predefined network services. In this application, Netlet based services embedded with intelligence to support server selection are deployed by servers close to potential client communities to setup dynamic service decision points within the network. An anycast address is used to identify available distributed decision points in the network. Each service decision point transparently directs client requests to the best performing server based on its in-built intelligence supported by real-time measurements from probes sent by the Netlet to each server. It is shown that the resulting system provides a client-side server selection solution which is server-customisable, scalable and fault transparent

Experimental Evaluation of Wireless Mesh Networks: A Case Study and Comparison

Price of WiFi devices has decreased dramatically in recent years, while new standards, as 802.11n, have multiplied its performance. This has fostered the deployment of Wireless Mesh networks (WMN), putting into practice concepts evolved from more than a decade of research in Ad Hoc networks. Nevertheless, evolution of WMN it is in its infancy, as shows the growing and diverse number of scenarios where WMN are being deployed. In these paper we analyze a particular case study of a Wireless Community Mesh Network, and we compare it with a selected experimental WMN studies found in the literature

Inferring Internet AS Relationships Based on BGP Routing Policies

The type of business relationships between the Internet autonomous systems (AS) determines the BGP inter-domain routing. Previous works on inferring AS relationships relied on the connectivity information between ASes. In this paper we infer AS relationships by analysing the routing polices of ASes encoded in the BGP attributes Communities and the Locpref. We accumulate BGP data from RouteViews, RIPE RIS and the public Route Servers in August 2010 and February 2011. Based on the routing policies extracted from data of the two BGP attributes, we obtain AS relationships for 39% links in our data, which include all links among the Tier-1 ASes and most links between Tier-1 and Tier-2 ASes. We also reveal a number of special AS relationships, namely the hybrid relationship, the partial-transit relationship, the indirect peering relationship and the backup links. These special relationships are relevant to a better understanding of the Internet routing. Our work provides a profound methodological progress for inferring the AS relationships.Comment: 8 pages and 3 figure