Fixed points of Boolean networks, guessing graphs, and coding theory

n this paper, we are interested in the number of fixed points of functions $f:A^n\to A^n$ over a finite alphabet $A$ defined on a given signed digraph $D$. We first use techniques from network coding to derive some lower bounds on the number of fixed points that only depends on $D$. We then discover relationships between the number of fixed points of $f$ and problems in coding theory, especially the design of codes for the asymmetric channel. Using these relationships, we derive upper and lower bounds on the number of fixed points, which significantly improve those given in the literature. We also unveil some interesting behavior of the number of fixed points of functions with a given signed digraph when the alphabet varies. We finally prove that signed digraphs with more (disjoint) positive cycles actually do not necessarily have functions with more fixed points

Logic Integer Programming Models for Signaling Networks

We propose a static and a dynamic approach to model biological signaling networks, and show how each can be used to answer relevant biological questions. For this we use the two different mathematical tools of Propositional Logic and Integer Programming. The power of discrete mathematics for handling qualitative as well as quantitative data has so far not been exploited in Molecular Biology, which is mostly driven by experimental research, relying on first-order or statistical models. The arising logic statements and integer programs are analyzed and can be solved with standard software. For a restricted class of problems the logic models reduce to a polynomial-time solvable satisfiability algorithm. Additionally, a more dynamic model enables enumeration of possible time resolutions in poly-logarithmic time. Computational experiments are included

Asynchronous simulation of Boolean networks by monotone Boolean networks

International audienceWe prove that the fully asynchronous dynamics of a Boolean network f : {0, 1}^n → {0, 1}^n without negative loop can be simulated, in a very specific way, by a monotone Boolean network with 2n components. We then use this result to prove that, for every even n, there exists a monotone Boolean network f : {0, 1}^n → {0, 1}^n , an initial configuration x and a fixed point y of f such that: (i) y can be reached from x with a fully asynchronous updating strategy, and (ii) all such strategies contains at least 2^{n/2} updates. This contrasts with the following known property: if f : {0, 1}^n → {0, 1}^n is monotone, then, for every initial configuration x, there exists a fixed point y such that y can be reached from x with a fully asynchronous strategy that contains at most n updates

Complexity of fixed point counting problems in Boolean Networks

A Boolean network (BN) with $n$ components is a discrete dynamical system described by the successive iterations of a function $f:\{0,1\}^n \to \{0,1\}^n$. This model finds applications in biology, where fixed points play a central role. For example, in genetic regulations, they correspond to cell phenotypes. In this context, experiments reveal the existence of positive or negative influences among components: component $i$ has a positive (resp. negative) influence on component $j$ meaning that $j$ tends to mimic (resp. negate) $i$. The digraph of influences is called signed interaction digraph (SID), and one SID may correspond to a large number of BNs (which is, in average, doubly exponential according to $n$). The present work opens a new perspective on the well-established study of fixed points in BNs. When biologists discover the SID of a BN they do not know, they may ask: given that SID, can it correspond to a BN having at least/at most $k$ fixed points? Depending on the input, we prove that these problems are in $\textrm{P}$ or complete for $\textrm{NP}$, $\textrm{NP}^{\textrm{NP}}$, \textrm{NP}^{\textrm{#P}} or $\textrm{NEXPTIME}$. In particular, we prove that it is $\textrm{NP}$-complete (resp. $\textrm{NEXPTIME}$-complete) to decide if a given SID can correspond to a BN having at least two fixed points (resp. no fixed point).Comment: 43 page