Accurate Feature Extraction and Control Point Correction for Camera Calibration with a Mono-Plane Target

The paper addresses two problems related to 3D camera calibration using a single mono-plane calibration target with circular control marks. The first problem is how to compute accurately the locations of the features (ellipses) in images of the target. Since the structure of the control marks is known beforehand, we propose to use a shape-specific searching technique to find the optimal locations of the features. Our experiments have shown this technique generates more accurate feature locations than the state-of-the-art ellipse extraction methods. The second problem is how to refine the control mark locations with unknown manufacturing errors. We demonstrate in a case study, where the control marks are laser printed on a A4 paper, that the manufacturing errors of the control marks can be compensated to a good extent so that the remaining calibration errors are reduced significantly. 1

Pigment Melanin: Pattern for Iris Recognition

Recognition of iris based on Visible Light (VL) imaging is a difficult problem because of the light reflection from the cornea. Nonetheless, pigment melanin provides a rich feature source in VL, unavailable in Near-Infrared (NIR) imaging. This is due to biological spectroscopy of eumelanin, a chemical not stimulated in NIR. In this case, a plausible solution to observe such patterns may be provided by an adaptive procedure using a variational technique on the image histogram. To describe the patterns, a shape analysis method is used to derive feature-code for each subject. An important question is how much the melanin patterns, extracted from VL, are independent of iris texture in NIR. With this question in mind, the present investigation proposes fusion of features extracted from NIR and VL to boost the recognition performance. We have collected our own database (UTIRIS) consisting of both NIR and VL images of 158 eyes of 79 individuals. This investigation demonstrates that the proposed algorithm is highly sensitive to the patterns of cromophores and improves the iris recognition rate.Comment: To be Published on Special Issue on Biometrics, IEEE Transaction on Instruments and Measurements, Volume 59, Issue number 4, April 201

Laser Ultrasound Inspection Based on Wavelet Transform and Data Clustering for Defect Estimation in Metallic Samples

Laser-generated ultrasound is a modern non-destructive testing technique. It has been investigated over recent years as an alternative to classical ultrasonic methods, mainly in industrial maintenance and quality control procedures. In this study, the detection and reconstruction of internal defects in a metallic sample is performed by means of a time-frequency analysis of ultrasonic waves generated by a laser-induced thermal mechanism. In the proposed methodology, we used wavelet transform due to its multi-resolution time frequency characteristics. In order to isolate and estimate the corresponding time of flight of eventual ultrasonic echoes related to internal defects, a density-based spatial clustering was applied to the resulting time frequency maps. Using the laser scan beam’s position, the ultrasonic transducer’s location and the echoes’ arrival times were determined, the estimation of the defect’s position was carried out afterwards. Finally, clustering algorithms were applied to the resulting geometric solutions from the set of the laser scan points which was proposed to obtain a two-dimensional projection of the defect outline over the scan plane. The study demonstrates that the proposed method of wavelet transform ultrasonic imaging can be effectively applied to detect and size internal defects without any reference information, which represents a valuable outcome for various applications in the industry. View Full-TextPeer ReviewedPostprint (published version

Electrowetting-Based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting is the effect by which the contact angle of a droplet exposed to a surface charge is modified. Electrowetting-on-dielectric (EWOD) exploits the dielectric properties of thin insulator films to enhance the charge density and hence boost the electrowetting effect. The presence of charges results in an electrically induced spreading of the droplet which permits purposeful manipulation across a hydrophobic surface. Here, we demonstrate EWOD-based protocol for sample processing and detection of four categories of antigens, using an automated surface actuation platform, via two variations of an Enzyme-Linked Immunosorbent Assay (ELISA) methods. The ELISA is performed on magnetic beads with immobilized primary antibodies which can be selected to target a specific antigen. An antibody conjugated to HRP binds to the antigen and is mixed with H 2O 2/Luminol for quantification of the captured pathogens. Assay completion times of between 6 and 10 min were achieved, whilst minuscule volumes of reagents were utilized.Peer reviewe