To Clone or Not To Clone: Method Analysis for Retrieving Consensus Sequences In Ancient DNA Samples

The challenges associated with the retrieval and authentication of ancient DNA (aDNA) evidence are principally due to post-mortem damage which makes ancient samples particularly prone to contamination from “modern” DNA sources. The necessity for authentication of results has led many aDNA researchers to adopt methods considered to be “gold standards” in the field, including cloning aDNA amplicons as opposed to directly sequencing them. However, no standardized protocol has emerged regarding the necessary number of clones to sequence, how a consensus sequence is most appropriately derived, or how results should be reported in the literature. In addition, there has been no systematic demonstration of the degree to which direct sequences are affected by damage or whether direct sequencing would provide disparate results from a consensus of clones

A statistical mechanics approach to autopoietic immune networks

The aim of this work is to try to bridge over theoretical immunology and disordered statistical mechanics. Our long term hope is to contribute to the development of a quantitative theoretical immunology from which practical applications may stem. In order to make theoretical immunology appealing to the statistical physicist audience we are going to work out a research article which, from one side, may hopefully act as a benchmark for future improvements and developments, from the other side, it is written in a very pedagogical way both from a theoretical physics viewpoint as well as from the theoretical immunology one. Furthermore, we have chosen to test our model describing a wide range of features of the adaptive immune response in only a paper: this has been necessary in order to emphasize the benefit available when using disordered statistical mechanics as a tool for the investigation. However, as a consequence, each section is not at all exhaustive and would deserve deep investigation: for the sake of completeness, we restricted details in the analysis of each feature with the aim of introducing a self-consistent model.Comment: 22 pages, 14 figur

`Human clones talk about their lives': Media representations of assisted reproductive and biogenetic technologies

This article examines New Zealand print media representations of assisted reproductive and related biogenetic technologies, conceptualized as the products of a concordance of interest between media workers and reproductive specialists, biogenetic scientists and consumers. Such concordance is evident in the predominant use of media frames of anecdotal personalization and technoboosterism, which typically amplify the voices of proponents of emerging technologies while marginalizing and delegitimizing counterdiscourses. Thus, the perspectives of consumers and 'expert' sources are privileged at the expense of a more balanced assessment of the value and social, ethical, legal and health implications of assisted reproductive and related biogenetic technologies. Source dependence also detracts from much-needed recognition of the professional and financial interests at stake in the growing privatization and commercialization of these technologies, and in the local context potentially undermines journalistic independence and integrity

Containing Systemic Risk

Systemic risk refers to the risk of financial system breakdown due to linkages between institutions. This risk cannot be assessed by looking at how individual institutions manage risks but instead requires a full understanding of how the system as a whole operates. At present, the data available to central banks and financial regulators are not at all adequate for the task of assessing systemic risk and the new European Systemic Risk Board needs to address this issue. There is a lot of exciting ongoing research devoted to measuring systemic risk and providing signals to regulators as to when and where they should intervene. However, the tools being developed are still limited in their usefulness. More pressing than the development of these tools is the development and implementation of policy measures to make the financial system more robust. These measures should include higher capital ratios, limits on non-core funding and redesigning financial systems to be less complex.Financial Risk,Systemic Risk,Banking