6,528 research outputs found

    Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening

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    New secondary mesenchyme specific genes, expressed exclusively in pigment cells, were isolated from sea urchin embryos using a differential screening of a macroarray cDNA library. The comparison was performed between mRNA populations of embryos having an expansion of the endo-mesodermal territory and embryos blocked in secondary mesenchyme specification. To be able to isolate transcripts with a prevalence down to five copies per cell, a subtractive hybridization procedure was employed. About 400 putative positive clones were identified and sequenced from the 5' end. Gene expression analysis was carried out on a subset of 66 clones with real time quantitative PCR and 40 clones were positive. This group of clones contained sequences highly similar to: the transcription factor glial cells missing (gcm); the polyketide synthase gene cluster (pks-gc); three different members of the flavin-containing monooxygenase gene family (fmo); and a sulfotransferase gene (sult). Using whole mount in situ hybridization, it was shown that these genes are specifically expressed in pigment cells. A functional analysis of the S. purpuratus pks and of one S. purpuratus fmo was carried out using antisense technology and it was shown that their expression is necessary for the biosynthesis of the sea urchin pigment echinochrome. The results suggest that S. purpuratus pks, fmo and sult could belong to a differentiation gene battery of pigment cells

    Digital Color Imaging

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    This paper surveys current technology and research in the area of digital color imaging. In order to establish the background and lay down terminology, fundamental concepts of color perception and measurement are first presented us-ing vector-space notation and terminology. Present-day color recording and reproduction systems are reviewed along with the common mathematical models used for representing these devices. Algorithms for processing color images for display and communication are surveyed, and a forecast of research trends is attempted. An extensive bibliography is provided

    Estimation from quantized Gaussian measurements: when and how to use dither

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    Subtractive dither is a powerful method for removing the signal dependence of quantization noise for coarsely quantized signals. However, estimation from dithered measurements often naively applies the sample mean or midrange, even when the total noise is not well described with a Gaussian or uniform distribution. We show that the generalized Gaussian distribution approximately describes subtractively dithered, quantized samples of a Gaussian signal. Furthermore, a generalized Gaussian fit leads to simple estimators based on order statistics that match the performance of more complicated maximum likelihood estimators requiring iterative solvers. The order statistics-based estimators outperform both the sample mean and midrange for nontrivial sums of Gaussian and uniform noise. Additional analysis of the generalized Gaussian approximation yields rules of thumb for determining when and how to apply dither to quantized measurements. Specifically, we find subtractive dither to be beneficial when the ratio between the Gaussian standard deviation and quantization interval length is roughly less than one-third. When that ratio is also greater than 0.822/K^0.930 for the number of measurements K > 20, estimators we present are more efficient than the midrange.https://arxiv.org/abs/1811.06856Accepted manuscrip

    The combline filter and phase-lock loop. A new technique to improve FM television reception

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    Development and performance of combline filter phase locked loop combination for television receptio

    Identification of Evolving Rule-based Models.

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    An approach to identification of evolving fuzzy rule-based (eR) models is proposed. eR models implement a method for the noniterative update of both the rule-base structure and parameters by incremental unsupervised learning. The rule-base evolves by adding more informative rules than those that previously formed the model. In addition, existing rules can be replaced with new rules based on ranking using the informative potential of the data. In this way, the rule-base structure is inherited and updated when new informative data become available, rather than being completely retrained. The adaptive nature of these evolving rule-based models, in combination with the highly transparent and compact form of fuzzy rules, makes them a promising candidate for modeling and control of complex processes, competitive to neural networks. The approach has been tested on a benchmark problem and on an air-conditioning component modeling application using data from an installation serving a real building. The results illustrate the viability and efficiency of the approach. (c) IEEE Transactions on Fuzzy System

    Synthesizer Parameter Approximation by Deep Learning

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    Synthesizers have been an essential tool for composers of any style of music including computer generated sound. They allow for an expansion in timbral variety to the orchestration of a piece of music or sound scape. Sound designers are trained to be able to recreate a timbre in their head using a synthesizer. This works well for simple sounds but becomes more difficult as the number of parameters required to produce a specific timbre increase. The goal of this research project is to formulate a method for synthesizers to approximate a timbre given an input audio sample using deep learning. The synthesizer should be able to modify its settings (oscillators, filters, LFOs, effects, etc.) to produce an audio signal as close to the input sample as possible. A cost function will measure the difference between the outputted audio signal from the learned synthesizer parameters and the original audio signal that is being mimicked
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