Small secreted proteins enable biofilm development in the cyanobacterium Synechococcus elongatus.

Small proteins characterized by a double-glycine (GG) secretion motif, typical of secreted bacterial antibiotics, are encoded by the genomes of diverse cyanobacteria, but their functions have not been investigated to date. Using a biofilm-forming mutant of Synechococcus elongatus PCC 7942 and a mutational approach, we demonstrate the involvement of four small secreted proteins and their GG-secretion motifs in biofilm development. These proteins are denoted EbfG1-4 (enable biofilm formation with a GG-motif). Furthermore, the conserved cysteine of the peptidase domain of the Synpcc7942_1133 gene product (dubbed PteB for peptidase transporter essential for biofilm) is crucial for biofilm development and is required for efficient secretion of the GG-motif containing proteins. Transcriptional profiling of ebfG1-4 indicated elevated transcript levels in the biofilm-forming mutant compared to wild type (WT). However, these transcripts decreased, acutely but transiently, when the mutant was cultured in extracellular fluids from a WT culture, and biofilm formation was inhibited. We propose that WT cells secrete inhibitor(s) that suppress transcription of ebfG1-4, whereas secretion of the inhibitor(s) is impaired in the biofilm-forming mutant, leading to synthesis and secretion of EbfG1-4 and supporting the formation of biofilms

Potential applications of geospatial information systems for planning and managing aged care services in Australia

[Abstract]: This paper discusses the potential applications of Geospatial Information Technology (GITs) to assist in planning and managing aged care programs in Australia. Aged care is complex due to the numbers of participants at all levels of including planning of services, investing in capacity, funding, providing services, auditing, monitoring quality, and in accessing and using facilities and services. There is a vast array of data spread across the entities that are joined to aged care. The decision-making process for investment in capacity and service provision might be aided by technology including GIT. This is also expected to assist in managing and analysing the vast amount of demographic, geographic, socio-economic and behavioral data that might indicate current and future demand for services the aged and frail-aged population. Mapping spatio-temporal changes in near real time can assist in the successful planning and management of aged care programs. Accurate information on the location of aged care services centres and mapping the special needs of clients and their service needs may assist in monitoring access to services and assist in identifying areas where there are logistic challenges for accessing services to meet needs. GIT can also identifying migrations of aged people and of the cohorts of the population who are likely to be the next wave of clients for aged care services. GITs include remote sensing, geographic information systems (GIS) and global positioning systems (GPS) technologies, which can be used to develop a user friendly digital system for monitoring, evaluating and planning aged care and community care in Australia. Whilst remote sensing data can provide current spatiotemporal inventory of features such as locations of carer services, infrastructure, on a consistent and continuous coordinate system, a GIS can assist in storing, cross analysing, modeling and mapping of spatial data pertaining to the needs of the older people. GITs can assist in the development of a single one-stop digital database which will prove a better model for managing aged care in Australia. GIT will also be a component of technologies such as activity monitors to provide tracking functionality. This will assist in tracking dementia sufferers who may be prone to wandering and be exposed to risk