Proteomic Analysis of a Noninvasive Human Model of Acute Inflammation and Its Resolution: The Twenty-one Day Gingivitis Model

The 21-day experimental gingivitis model, an established noninvasive model of inflammation in response to increasing bacterial accumulation in humans, is designed to enable the study of both the induction and resolution of inflammation. Here, we have analyzed gingival crevicular fluid, an oral fluid comprising a serum transudate and tissue exudates, by LC−MS/MS using Fourier transform ion cyclotron resonance mass spectrometry and iTRAQ isobaric mass tags, to establish meta-proteomic profiles of inflammation-induced changes in proteins in healthy young volunteers. Across the course of experimentally induced gingivitis, we identified 16 bacterial and 186 human proteins. Although abundances of the bacterial proteins identified did not vary temporally, Fusobacterium outer membrane proteins were detected. Fusobacterium species have previously been associated with periodontal health or disease. The human proteins identified spanned a wide range of compartments (both extracellular and intracellular) and functions, including serum proteins, proteins displaying antibacterial properties, and proteins with functions associated with cellular transcription, DNA binding, the cytoskeleton, cell adhesion, and cilia. PolySNAP3 clustering software was used in a multilayered analytical approach. Clusters of proteins that associated with changes to the clinical parameters included neuronal and synapse associated proteins

Carrier mode selective working point and side band imbalance in LIGO I

In gravitational wave interferometers, the input laser beam is phase modulated to generate radio-frequency side bands that are used to lock the cavities. The mechanism is the following: the frequency of the side bands and the carrier is chosen in such a way that their response to small changes of the longitudinal degrees of freedom is different. This difference is therefore monitored and it serves as an error signal for controlling the optical cavity lengths, as they are linearly related to the set of observed phases between carrier and side bands. Among the others, one longitudinal degree of freedom is optimally sensitive to the space-time distortions propagating through the cosmos, as predicted by the general theory of relativity. The observation of the astrophysical signal relies on the measurement of that specific degree of freedom. The entire problem is more complex when the transverse degrees of freedom are taken into account, because the relative phase between the fields also depends on their overlap. In order to establish an unambiguous relation between length changes and phase measurements, there must be one circulating optical mode and the only difference between carrier and side bands must be their amplitude. We will show that the variability of the transverse degrees of freedom and their different actions on carrier and side band fields puts a severe limit on this assumption. Unless the system is made of perfect and perfectly matched optical cavities, it is never governed by one unique coherent state and any adjustment of the optical lengths results from a compromise between the lengths that are optimal for the carrier field and the side band ones. Such a compromise alters the correspondence between error signals and cavity lengths, calculated in the one-dimensional treatment. We assess the strength of this effect and relate it to the sensitivity of the instrument (which relies on the reconstruction of that correspondence) in realistic circumstances

OAM multiple transmission using uniform circular arrays: numerical modeling and experimental verification with two digital television signals

In this work we present the outcomes of a radio-frequency OAM transmission between two antenna arrays performed in a real-world context. The analysis is supplemented by deep simulative investigations able to provide both a preliminary overview of the experimental scenario and a posteriori validation of the achieved results. As a first step, the far-field OAM communication link is tested at various frequencies and the corresponding link budget is studied by means of an angular scan generated by the rotation of the receiving system. Then, on the same site, two digital television signals encoded as OAM modes ($\ell$=1 and $\ell$=-1) are simultaneously transmitted at a common frequency of 198.5 MHz with good mode insulation.Comment: 16 pages, 14 figure

Probing Ultrafast Dynamics with Time-resolved Multi-dimensional Coincidence Imaging: Butadiene

Time-resolved coincidence imaging of photoelectrons and photoions represents the most complete experimental measurement of ultrafast excited state dynamics, a multi-dimensional measurement for a multi-dimensional problem. Here we present the experimental data from recent coincidence imaging experiments, undertaken with the aim of gaining insight into the complex ultrafast excited-state dynamics of 1,3-butadiene initiated by absorption of 200 nm light. We discuss photoion and photoelectron mappings of increasing dimensionality, and focus particularly on the time-resolved photoelectron angular distributions (TRPADs), expected to be a sensitive probe of the electronic evolution of the excited state and to provide significant information beyond the time-resolved photoelectron spectrum (TRPES). Complex temporal behaviour is observed in the TRPADs, revealing their sensitivity to the dynamics while also emphasising the difficulty of interpretation of these complex observables. From the experimental data some details of the wavepacket dynamics are discerned relatively directly, and we make some tentative comparisons with existing ab initio calculations in order to gain deeper insight into the experimental measurements; finally, we sketch out some considerations for taking this comparison further in order to bridge the gap between experiment and theory.Comment: 18 pages, 10 figures. Pre-print of JMO submissio

Detection of Sparse Anomalies in High-Dimensional Network Telescope Signals

Network operators and system administrators are increasingly overwhelmed with incessant cyber-security threats ranging from malicious network reconnaissance to attacks such as distributed denial of service and data breaches. A large number of these attacks could be prevented if the network operators were better equipped with threat intelligence information that would allow them to block or throttle nefarious scanning activities. Network telescopes or "darknets" offer a unique window into observing Internet-wide scanners and other malicious entities, and they could offer early warning signals to operators that would be critical for infrastructure protection and/or attack mitigation. A network telescope consists of unused or "dark" IP spaces that serve no users, and solely passively observes any Internet traffic destined to the "telescope sensor" in an attempt to record ubiquitous network scanners, malware that forage for vulnerable devices, and other dubious activities. Hence, monitoring network telescopes for timely detection of coordinated and heavy scanning activities is an important, albeit challenging, task. The challenges mainly arise due to the non-stationarity and the dynamic nature of Internet traffic and, more importantly, the fact that one needs to monitor high-dimensional signals (e.g., all TCP/UDP ports) to search for "sparse" anomalies. We propose statistical methods to address both challenges in an efficient and "online" manner; our work is validated both with synthetic data as well as real-world data from a large network telescope

Nanomechanical single-photon routing

The merger between integrated photonics and quantum optics promises new opportunities within photonic quantum technology with the very significant progress on excellent photon-emitter interfaces and advanced optical circuits. A key missing functionality is rapid circuitry reconfigurability that ultimately does not introduce loss or emitter decoherence, and operating at a speed matching the photon generation and quantum memory storage time of the on-chip quantum emitter. This ambitious goal requires entirely new active quantum-photonic devices by extending the traditional approaches to reconfigurability. Here, by merging nano-optomechanics and deterministic photon-emitter interfaces we demonstrate on-chip single-photon routing with low loss, small device footprint, and an intrinsic time response approaching the spin coherence time of solid-state quantum emitters. The device is an essential building block for constructing advanced quantum photonic architectures on-chip, towards, e.g., coherent multi-photon sources, deterministic photon-photon quantum gates, quantum repeater nodes, or scalable quantum networks.Comment: 7 pages, 3 figures, supplementary informatio

A time-dependent Schr\"odinger equation for molecular core-hole dynamics

X-ray spectroscopy is an important tool for the investigation of matter. X rays primarily interact with inner-shell electrons creating core (inner-shell) holes that will decay on the time scale of attoseconds to few femtoseconds through electron relaxations involving the emission of a photon or an electron. The advent of femtosecond x-ray pulses expands x-ray spectroscopy to the time domain and will eventually allow the control of core-hole population on timescales comparable to core-vacancy lifetimes. For both cases, a theoretical approach that accounts for the x-ray interaction while the electron relaxations occur is required. Here we describe a time-dependent framework, based on solving the time-dependent Schr\"odinger equation, that is suitable for describing the induced electron and nuclear dynamics

Predicting the statistics of wave transport through chaotic cavities by the Random Coupling Model: a review and recent progress

In this review, a model (the Random Coupling Model) that gives a statistical description of the coupling of radiation into and out of large enclosures through localized and/or distributed channels is presented. The Random Coupling Model combines both deterministic and statistical phenomena. The model makes use of wave chaos theory to extend the classical modal description of the cavity fields in the presence of boundaries that lead to chaotic ray trajectories. The model is based on a clear separation between the universal statistical behavior of the isolated chaotic system, and the deterministic coupling channel characteristics. Moreover, the ability of the random coupling model to describe interconnected cavities, aperture coupling, and the effects of short ray trajectories is discussed. A relation between the random coupling model and other formulations adopted in acoustics, optics, and statistical electromagnetics, is examined. In particular, a rigorous analogy of the random coupling model with the Statistical Energy Analysis used in acoustics is presented.Comment: 32 pages, 9 figures, submitted to 'Wave Motion', special issue 'Innovations in Wave Model