Fuzzy-based Propagation of Prior Knowledge to Improve Large-Scale Image Analysis Pipelines

Many automatically analyzable scientific questions are well-posed and offer a variety of information about the expected outcome a priori. Although often being neglected, this prior knowledge can be systematically exploited to make automated analysis operations sensitive to a desired phenomenon or to evaluate extracted content with respect to this prior knowledge. For instance, the performance of processing operators can be greatly enhanced by a more focused detection strategy and the direct information about the ambiguity inherent in the extracted data. We present a new concept for the estimation and propagation of uncertainty involved in image analysis operators. This allows using simple processing operators that are suitable for analyzing large-scale 3D+t microscopy images without compromising the result quality. On the foundation of fuzzy set theory, we transform available prior knowledge into a mathematical representation and extensively use it enhance the result quality of various processing operators. All presented concepts are illustrated on a typical bioimage analysis pipeline comprised of seed point detection, segmentation, multiview fusion and tracking. Furthermore, the functionality of the proposed approach is validated on a comprehensive simulated 3D+t benchmark data set that mimics embryonic development and on large-scale light-sheet microscopy data of a zebrafish embryo. The general concept introduced in this contribution represents a new approach to efficiently exploit prior knowledge to improve the result quality of image analysis pipelines. Especially, the automated analysis of terabyte-scale microscopy data will benefit from sophisticated and efficient algorithms that enable a quantitative and fast readout. The generality of the concept, however, makes it also applicable to practically any other field with processing strategies that are arranged as linear pipelines.Comment: 39 pages, 12 figure

Multigranularity Representations for Human Inter-Actions: Pose, Motion and Intention

Tracking people and their body pose in videos is a central problem in computer vision. Standard tracking representations reason about temporal coherence of detected people and body parts. They have difficulty tracking targets under partial occlusions or rare body poses, where detectors often fail, since the number of training examples is often too small to deal with the exponential variability of such configurations. We propose tracking representations that track and segment people and their body pose in videos by exploiting information at multiple detection and segmentation granularities when available, whole body, parts or point trajectories. Detections and motion estimates provide contradictory information in case of false alarm detections or leaking motion affinities. We consolidate contradictory information via graph steering, an algorithm for simultaneous detection and co-clustering in a two-granularity graph of motion trajectories and detections, that corrects motion leakage between correctly detected objects, while being robust to false alarms or spatially inaccurate detections. We first present a motion segmentation framework that exploits long range motion of point trajectories and large spatial support of image regions. We show resulting video segments adapt to targets under partial occlusions and deformations. Second, we augment motion-based representations with object detection for dealing with motion leakage. We demonstrate how to combine dense optical flow trajectory affinities with repulsions from confident detections to reach a global consensus of detection and tracking in crowded scenes. Third, we study human motion and pose estimation. We segment hard to detect, fast moving body limbs from their surrounding clutter and match them against pose exemplars to detect body pose under fast motion. We employ on-the-fly human body kinematics to improve tracking of body joints under wide deformations. We use motion segmentability of body parts for re-ranking a set of body joint candidate trajectories and jointly infer multi-frame body pose and video segmentation. We show empirically that such multi-granularity tracking representation is worthwhile, obtaining significantly more accurate multi-object tracking and detailed body pose estimation in popular datasets

Object Tracking

Object tracking consists in estimation of trajectory of moving objects in the sequence of images. Automation of the computer object tracking is a difficult task. Dynamics of multiple parameters changes representing features and motion of the objects, and temporary partial or full occlusion of the tracked objects have to be considered. This monograph presents the development of object tracking algorithms, methods and systems. Both, state of the art of object tracking methods and also the new trends in research are described in this book. Fourteen chapters are split into two sections. Section 1 presents new theoretical ideas whereas Section 2 presents real-life applications. Despite the variety of topics contained in this monograph it constitutes a consisted knowledge in the field of computer object tracking. The intention of editor was to follow up the very quick progress in the developing of methods as well as extension of the application