Two-Dimensional Gel Electrophoresis Image Registration Using Block-Matching Techniques and Deformation Models

[Abstract] Block-matching techniques have been widely used in the task of estimating displacement in medical images, and they represent the best approach in scenes with deformable structures such as tissues, fluids, and gels. In this article, a new iterative block-matching technique—based on successive deformation, search, fitting, filtering, and interpolation stages—is proposed to measure elastic displacements in two-dimensional polyacrylamide gel electrophoresis (2D–PAGE) images. The proposed technique uses different deformation models in the task of correlating proteins in real 2D electrophoresis gel images, obtaining an accuracy of 96.6% and improving the results obtained with other techniques. This technique represents a general solution, being easy to adapt to different 2D deformable cases and providing an experimental reference for block-matching algorithms.Galicia. Consellería de Economía e Industria; 10MDS014CTGalicia. Consellería de Economía e Industria; 10SIN105004PRInstituto de Salud Carlos III; PI13/0028

Spontaneous Subtle Expression Detection and Recognition based on Facial Strain

Optical strain is an extension of optical flow that is capable of quantifying subtle changes on faces and representing the minute facial motion intensities at the pixel level. This is computationally essential for the relatively new field of spontaneous micro-expression, where subtle expressions can be technically challenging to pinpoint. In this paper, we present a novel method for detecting and recognizing micro-expressions by utilizing facial optical strain magnitudes to construct optical strain features and optical strain weighted features. The two sets of features are then concatenated to form the resultant feature histogram. Experiments were performed on the CASME II and SMIC databases. We demonstrate on both databases, the usefulness of optical strain information and more importantly, that our best approaches are able to outperform the original baseline results for both detection and recognition tasks. A comparison of the proposed method with other existing spatio-temporal feature extraction approaches is also presented.Comment: 21 pages (including references), single column format, accepted to Signal Processing: Image Communication journa

Robust Temporally Coherent Laplacian Protrusion Segmentation of 3D Articulated Bodies

In motion analysis and understanding it is important to be able to fit a suitable model or structure to the temporal series of observed data, in order to describe motion patterns in a compact way, and to discriminate between them. In an unsupervised context, i.e., no prior model of the moving object(s) is available, such a structure has to be learned from the data in a bottom-up fashion. In recent times, volumetric approaches in which the motion is captured from a number of cameras and a voxel-set representation of the body is built from the camera views, have gained ground due to attractive features such as inherent view-invariance and robustness to occlusions. Automatic, unsupervised segmentation of moving bodies along entire sequences, in a temporally-coherent and robust way, has the potential to provide a means of constructing a bottom-up model of the moving body, and track motion cues that may be later exploited for motion classification. Spectral methods such as locally linear embedding (LLE) can be useful in this context, as they preserve "protrusions", i.e., high-curvature regions of the 3D volume, of articulated shapes, while improving their separation in a lower dimensional space, making them in this way easier to cluster. In this paper we therefore propose a spectral approach to unsupervised and temporally-coherent body-protrusion segmentation along time sequences. Volumetric shapes are clustered in an embedding space, clusters are propagated in time to ensure coherence, and merged or split to accommodate changes in the body's topology. Experiments on both synthetic and real sequences of dense voxel-set data are shown. This supports the ability of the proposed method to cluster body-parts consistently over time in a totally unsupervised fashion, its robustness to sampling density and shape quality, and its potential for bottom-up model constructionComment: 31 pages, 26 figure

Image processing for displacement measurements

Since the invention of photography humans have been using images to capture, store and analyse the act that they are interested in. With the developments in this field, assisted by better computers, it is possible to use image processing technology as an accurate method of analysis and measurement. Image processing's principal qualities are flexibility, adaptability and the ability to easily and quickly process a large amount of information. Successful examples of applications can be seen in several areas of human life, such as biomedical, industry, surveillance, military and mapping. This is so true that there are several Nobel prizes related to imaging. The accurate measurement of deformations, displacements, strain fields and surface defects are challenging in many material tests in Civil Engineering because traditionally these measurements require complex and expensive equipment, plus time consuming calibration. Image processing can be an inexpensive and effective tool for load displacement measurements. Using an adequate image acquisition system and taking advantage of the computation power of modern computers it is possible to accurately measure very small displacements with high precision. On the market there are already several commercial software packages. However they are commercialized at high cost. In this work block-matching algorithms will be used in order to compare the results from image processing with the data obtained with physical transducers during laboratory load tests. In order to test the proposed solutions several load tests were carried out in partnership with researchers from the Civil Engineering Department at Universidade Nova de Lisboa (UNL)

Progress in industrial photogrammetry by means of markerless solutions

174 p.La siguiente tesis está enfocada al desarrollo y uso avanzado de metodologías fotogramétrica sin dianas en aplicaciones industriales. La fotogrametría es una técnica de medición óptica 3D que engloba múltiples configuraciones y aproximaciones. En este estudio se han desarrollado procedimientos de medición, modelos y estrategias de procesamiento de imagen que van más allá que la fotogrametría convencional y buscan el emplear soluciones de otros campos de la visión artificial en aplicaciones industriales. Mientras que la fotogrametría industrial requiere emplear dianas artificiales para definir los puntos o elementos de interés, esta tesis contempla la reducción e incluso la eliminación de las dianas tanto pasivas como activas como alternativas prácticas. La mayoría de los sistemas de medida utilizan las dianas tanto para definir los puntos de control, relacionar las distintas perspectivas, obtener precisión, así como para automatizar las medidas. Aunque en muchas situaciones el empleo de dianas no sea restrictivo existen aplicaciones industriales donde su empleo condiciona y restringe considerablemente los procedimientos de medida empleados en la inspección. Un claro ejemplo es la verificación y control de calidad de piezas seriadas, o la medición y seguimiento de elementos prismáticos relacionados con un sistema de referencia determinado. Es en este punto donde la fotogrametría sin dianas puede combinarse o complementarse con soluciones tradicionales para tratar de mejorar las prestaciones actuales