288,508 research outputs found
Transversal inhomogeneities in dilute vibrofluidized granular fluids
The spontaneous symmetry breaking taking place in the direction perpendicular
to the energy flux in a dilute vibrofluidized granular system is investigated,
using both a hydrodynamic description and simulation methods. The latter
include molecular dynamics and direct Monte Carlo simulation of the Boltzmann
equation. A marginal stability analysis of the hydrodynamic equations, carried
out in the WKB approximation, is shown to be in good agreement with the
simulation results. The shape of the hydrodynamic profiles beyond the
bifurcation is discussed
Neural crest migration is driven by a few trailblazer cells with a unique molecular signature narrowly confined to the invasive front
Neural crest (NC) cell migration is crucial to the formation of peripheral tissues during vertebrate development. However, how NC cells respond to different microenvironments to maintain persistence of direction and cohesion in multicellular streams remains unclear. To address this, we profiled eight subregions of a typical cranial NC cell migratory stream. Hierarchical clustering showed significant differences in the expression profiles of the lead three subregions compared with newly emerged cells. Multiplexed imaging of mRNA expression using fluorescent hybridization chain reaction (HCR) quantitatively confirmed the expression profiles of lead cells. Computational modeling predicted that a small fraction of lead cells that detect directional information is optimal for successful stream migration. Single-cell profiling then revealed a unique molecular signature that is consistent and stable over time in a subset of lead cells within the most advanced portion of the migratory front, which we term trailblazers. Model simulations that forced a lead cell behavior in the trailing subpopulation predicted cell bunching near the migratory domain entrance. Misexpression of the trailblazer molecular signature by perturbation of two upstream transcription factors agreed with the in silico prediction and showed alterations to NC cell migration distance and stream shape. These data are the first to characterize the molecular diversity within an NC cell migratory stream and offer insights into how molecular patterns are transduced into cell behaviors
Rotation of the pre-stellar core L1689B
The search for the onset of star formation in pre-stellar cores has focussed
on the identification of an infall signature in the molecular line profiles of
tracer species. The classic infall signature is a double peaked line profile
with an asymmetry in the strength of the peaks such that the blue peak is
stronger. L1689B is a pre-stellar core and infall candidate but new JCMT HCO+
line profile data, presented here, confirms that both blue and red asymmetric
line profiles are present in this source. Moreover, a dividing line can be
drawn between the locations where each type of profile is found. It is argued
that it is unlikely that the line profiles can be interpreted with simple
models of infall or outflow and that rotation of the inner regions is the most
likely explanation. A rotational model is developed in detail with a new 3D
molecular line transport code and it is found that the best type of model is
one in which the rotational velocity profile is in between solid body and
Keplerian. It is firstly shown that red and blue asymmetric line profiles can
be generated with a rotation model entirely in the absence of any infall
motion. The model is then quantitively compared with the JCMT data and an
iteration over a range of parameters is performed to minmize the difference
between the data and model. The results indicate that rotation can dominate the
line profile shape even before the onset of infall.Comment: Accepted by MNRAS, 7 pages, 4 figure
Lateral-Pressure Profiles in Cholesterol-DPPC Bilayers
By means of atomistic molecular dynamics simulations, we study
cholesterol-DPPC (dipalmitoyl phosphatidylcholine) bilayers of different
composition, from pure DPPC bilayers to a 1:1 mixture of DPPC and cholesterol.
The lateral-pressure profiles through the bilayers are computed and separated
into contributions from the different components. We find that the pressure
inside the bilayer changes qualitatively for cholesterol concentrations of
about 20% or higher. The pressure profile then turns from a rather flat shape
into an alternating sequence of regions with large positive and negative
lateral pressure. The changes in the lateral-pressure profile are so
characteristic that specific interaction between cholesterol and molecules such
as membrane proteins mediated solely via the lateral-pressure profile might
become possible
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