In vitro expanded human CD4+CD25+ regulatory T cells suppress effector T cell proliferation.

Regulatory T cells (Tregs) have been shown to be critical in the balance between autoimmunity and tolerance and have been implicated in several human autoimmune diseases. However, the small number of Tregs in peripheral blood limits their therapeutic potential. Therefore, we developed a protocol that would allow for the expansion of Tregs while retaining their suppressive activity. We isolated CD4+CD25 hi cells from human peripheral blood and expanded them in vitro in the presence of anti-CD3 and anti-CD28 magnetic Xcyte Dynabeads and high concentrations of exogenous Interleukin (IL)-2. Tregs were effectively expanded up to 200-fold while maintaining surface expression of CD25 and other markers of Tregs: CD62L, HLA-DR, CCR6, and FOXP3. The expanded Tregs suppressed proliferation and cytokine secretion of responder PBMCs in co-cultures stimulated with anti-CD3 or alloantigen. Treg expansion is a critical first step before consideration of Tregs as a therapeutic intervention in patients with autoimmune or graft-versus-host disease

Stereoskope HD-Produktion: Grundlagen, Konzepte, Testergebnisse

Dieser Arbeitsbericht bündelt: 1. Nochmals die grundlegenden biologischen Aspekte und Grenzen der räumlichen Wahrnehmung von Menschen, 2. zeigt die historische Entwicklung der stereoskopischen Medien vom 18. Jahrhundert bis heute auf, 3. präsentiert eigenentwickelte technische Lösungsansätze für eine hochqualitative, stereoskopische HD-Videoproduktion, 4. beschreibt detailliert die Veränderungen, welche sich im gesamten Erstellungsprozess von der Planung bis zur Realisation ergeben und 5. versucht, gezielt Gestaltungsregeln für eine verstärkte, filmische Immersion herauszuarbeiten

JSC document index

The Johnson Space Center (JSC) document index is intended to provide a single source listing of all published JSC-numbered documents their authors, and the designated offices of prime responsibility (OPR's) by mail code at the time of publication. The index contains documents which have been received and processed by the JSC Technical Library as of January 13, 1988. Other JSC-numbered documents which are controlled but not available through the JSC Library are also listed