Generative Model with Coordinate Metric Learning for Object Recognition Based on 3D Models

Given large amount of real photos for training, Convolutional neural network shows excellent performance on object recognition tasks. However, the process of collecting data is so tedious and the background are also limited which makes it hard to establish a perfect database. In this paper, our generative model trained with synthetic images rendered from 3D models reduces the workload of data collection and limitation of conditions. Our structure is composed of two sub-networks: semantic foreground object reconstruction network based on Bayesian inference and classification network based on multi-triplet cost function for avoiding over-fitting problem on monotone surface and fully utilizing pose information by establishing sphere-like distribution of descriptors in each category which is helpful for recognition on regular photos according to poses, lighting condition, background and category information of rendered images. Firstly, our conjugate structure called generative model with metric learning utilizing additional foreground object channels generated from Bayesian rendering as the joint of two sub-networks. Multi-triplet cost function based on poses for object recognition are used for metric learning which makes it possible training a category classifier purely based on synthetic data. Secondly, we design a coordinate training strategy with the help of adaptive noises acting as corruption on input images to help both sub-networks benefit from each other and avoid inharmonious parameter tuning due to different convergence speed of two sub-networks. Our structure achieves the state of the art accuracy of over 50\% on ShapeNet database with data migration obstacle from synthetic images to real photos. This pipeline makes it applicable to do recognition on real images only based on 3D models.Comment: 14 page

System identification of gene regulatory networks for perturbation mitigation via feedback control

In Synthetic Biology, the idea of using feedback control for the mitigation of perturbations to gene regulatory networks due to disease and environmental disturbances is gaining popularity. To facilitate the design of such synthetic control circuits, a suitable model that captures the relevant dynamics of the gene regulatory network is essential. Traditionally, Michaelis-Menten models with Hill-type nonlinearities have often been used to model gene regulatory networks. Here, we show that such models are not suitable for the purposes of controller design, and propose an alternative formalism. Using tools from system identification, we show how to build so-called S-System models that capture the key dynamics of the gene regulatory network and are suitable for controller design. Using the identified S-System model, we design a genetic feedback controller for an example gene regulatory network with the objective of rejecting an external perturbation. Using a sine sweeping method, we show how the S-System model can be approximated by a second order linear transfer function and, based on this transfer function, we design our controller. Simulation results using the full nonlinear S-System model of the network show that the designed controller is able to mitigate the effect of external perturbations. Our findings highlight the usefulness of the S-System modelling formalism for the design of synthetic control circuits for gene regulatory networks

Characterization and mitigation of gene expression burden in mammalian cells

Despite recent advances in circuit engineering, the design of genetic networks in mammalian cells is still painstakingly slow and fraught with inexplicable failures. Here, we demonstrate that transiently expressed genes in mammalian cells compete for limited transcriptional and translational resources. This competition results in the coupling of otherwise independent exogenous and endogenous genes, creating a divergence between intended and actual function. Guided by a resource-aware mathematical model, we identify and engineer natural and synthetic miRNA-based incoherent feedforward loop (iFFL) circuits that mitigate gene expression burden. The implementation of these circuits features the use of endogenous miRNAs as elementary components of the engineered iFFL device, a versatile hybrid design that allows burden mitigation to be achieved across different cell-lines with minimal resource requirements. This study establishes the foundations for context-aware prediction and improvement of in vivo synthetic circuit performance, paving the way towards more rational synthetic construct design in mammalian cells

Revolutionising inverse design of magnesium alloys through generative adversarial networks

The utility of machine learning (ML) techniques in materials science has accelerated materials design and discovery. However, the accuracy of ML models - particularly deep neural networks - heavily relies on the quality and quantity of the training data. Data collection methods often have limitations arising from cost, difficulty, and resource-intensive human efforts. Thus, limited high-quality data, especially for novel materials, poses a significant challenge in developing reliable ML models. Generative adversarial networks (GANs) offer one solution to augment datasets through synthetic sample generation. The present work explores the application of GANs in magnesium (Mg) alloy design, by training two deep neural networks within the structure of a Wasserstein GAN to generate new (novel) alloys with desired mechanical properties. This data augmentation-based strategy contributes to model robustness, particularly in cases where traditional data collection is impractical. The approach presented may expedite Mg alloy development, through a GAN assisted inverse design approach.Comment: 23 pages, 4 figures, 2 tables, 1 Github repositor