31,411 research outputs found

    Non-common Path Aberration Correction in an Adaptive Optics Scanning Ophthalmoscope

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    The correction of non-common path aberrations (NCPAs) between the imaging and wavefront sensing channel in a confocal scanning adaptive optics ophthalmoscope is demonstrated. NCPA correction is achieved by maximizing an image sharpness metric while the confocal detection aperture is temporarily removed, effectively minimizing the monochromatic aberrations in the illumination path of the imaging channel. Comparison of NCPA estimated using zonal and modal orthogonal wavefront corrector bases provided wavefronts that differ by ~λ/20 in root-mean-squared (~λ/30 standard deviation). Sequential insertion of a cylindrical lens in the illumination and light collection paths of the imaging channel was used to compare image resolution after changing the wavefront correction to maximize image sharpness and intensity metrics. Finally, the NCPA correction was incorporated into the closed-loop adaptive optics control by biasing the wavefront sensor signals without reducing its bandwidth

    Compact hollow waveguide mid-infrared gas sensor for simultaneous measurements of ambient CO2 and water vapor

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    A compact, sensitive and stable hollow waveguide (HWG) mid-infrared gas sensor, based on gas absorption lines using wavelength modulation spectroscopy with a second harmonic (WMS-2f) detection scheme, was developed for simultaneous measurements of ambient CO 2 and water vapor. Optimization of the laser modulation parameters and pressure parameter in the HWG are performed to improve the strength of the WMS-2f signal and hence the detection limit, where 14.5-time for CO 2 and 8.5-time for water vapor improvement in system detection limit is achieved compared to those working at 1 atm. The stability of the sensor has been improved significantly by optimizing environmental disturbances, incoupling alignment of the HWG and laser scanning frequency. An Allan variance analysis shows detection limit of the developed sensor of ~3 ppmv for CO 2 and 0.018% for water vapor, which correspond to an absorbance of 2.4 Ă— 10 -5 and 2.7 Ă— 10 -5 , with a stability time of 160 s, respectively. Ambient CO 2 and water vapor measurement have been performed in two days in winter and spring separately. The measurement precision is further improved by applying a Kalman adaptive filter. The HWG gas sensor demonstrates the ability in environmental monitoring and the potential to be used in other areas, such as industrial production and biomedical diagnosis

    Light-sheet microscopy: a tutorial

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    This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.Peer ReviewedPostprint (published version

    The ESPRI project: astrometric exoplanet search with PRIMA I. Instrument description and performance of first light observations

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    The ESPRI project relies on the astrometric capabilities offered by the PRIMA facility of the Very Large Telescope Interferometer for the discovery and study of planetary systems. Our survey consists of obtaining high-precision astrometry for a large sample of stars over several years and to detect their barycentric motions due to orbiting planets. We present the operation principle, the instrument's implementation, and the results of a first series of test observations. A comprehensive overview of the instrument infrastructure is given and the observation strategy for dual-field relative astrometry is presented. The differential delay lines, a key component of the PRIMA facility which was delivered by the ESPRI consortium, are described and their performance within the facility is discussed. Observations of bright visual binaries are used to test the observation procedures and to establish the instrument's astrometric precision and accuracy. The data reduction strategy for astrometry and the necessary corrections to the raw data are presented. Adaptive optics observations with NACO are used as an independent verification of PRIMA astrometric observations. The PRIMA facility was used to carry out tests of astrometric observations. The astrometric performance in terms of precision is limited by the atmospheric turbulence at a level close to the theoretical expectations and a precision of 30 micro-arcseconds was achieved. In contrast, the astrometric accuracy is insufficient for the goals of the ESPRI project and is currently limited by systematic errors that originate in the part of the interferometer beamtrain which is not monitored by the internal metrology system. Our observations led to the definition of corrective actions required to make the facility ready for carrying out the ESPRI search for extrasolar planets.Comment: 32 pages, 39 figures, Accepted for publication in Astronomy and Astrophysic
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