Health and Diet in Upper Nubia through Climate and Political Change - A bioarchaeological investigation of health and living conditions at ancient Amara West between 1300 and 800BC

This thesis aims to investigate the impact of environmental and socio-political changes on health and living conditions in the ancient settlement of Amara West, Sudan (1300–800BC) through a diachronic comparison of selected indicators of disease and physiological stress on skeletal human remains. The town served as the administrative capital of the province Upper Nubia during the later phase of New Kingdom Egyptian occupation of Nubia (1300–1070BC). Despite the end of Egyptian control, settlement in the area continued until the 8th century. Palaeoenvironmental evidence from the region indicates that the period of occupation of the site further coincided with general climatic deterioration through increased aridification during the late 2nd and early 1st millennium BC. Whether these climatic and political changes would have had an affect on health and living conditions at Amara West is explored through comparing multiple markers of physiological stress and disease (stature, orbital changes, dental disease, evidence of nonspecific infection, respiratory diseases, endocranial changes, trauma, osteoarthritis) recorded through macroscopic examination of skeletal human remains from the New Kingdom (1300–1070BC, N=36) and post-New Kingdom period (1070–800BC, N=144). Analysis of stable oxygen and carbon isotopes was also included in the study. Applying a bio-cultural approach, interpretation of the results is complemented by contextual data drawn from ongoing research in the cemeteries, settlement and surrounding habitat. Despite limitations due to the bias in sample size, the systematic statistical comparison revealed several tentative trends such as decreasing stature, increased levels of osteoarthritis, dental pathologies, pulmonary diseases, post-cranial fractures and high levels of sub-adult mortality. Changes in stable oxygen isotope composition indicate increasingly arid conditions during the post-New Kingdom period. In light of palaeoenvironmental and isotopic data, the palaeopathological results may therefore reflect the health consequences of severe environmental changes as well as changes in settlement structure

Psr1p interacts with SUN/sad1p and EB1/mal3p to establish the bipolar spindle

Regular Abstracts - Sunday Poster Presentations: no. 382During mitosis, interpolar microtubules from two spindle pole bodies (SPBs) interdigitate to create an antiparallel microtubule array for accommodating numerous regulatory proteins. Among these proteins, the kinesin-5 cut7p/Eg5 is the key player responsible for sliding apart antiparallel microtubules and thus helps in establishing the bipolar spindle. At the onset of mitosis, two SPBs are adjacent to one another with most microtubules running nearly parallel toward the nuclear envelope, creating an unfavorable microtubule configuration for the kinesin-5 kinesins. Therefore, how the cell organizes the antiparallel microtubule array in the first place at mitotic onset remains enigmatic. Here, we show that a novel protein psrp1p localizes to the SPB and plays a key role in organizing the antiparallel microtubule array. The absence of psr1+ leads to a transient monopolar spindle and massive chromosome loss. Further functional characterization demonstrates that psr1p is recruited to the SPB through interaction with the conserved SUN protein sad1p and that psr1p physically interacts with the conserved microtubule plus tip protein mal3p/EB1. These results suggest a model that psr1p serves as a linking protein between sad1p/SUN and mal3p/EB1 to allow microtubule plus ends to be coupled to the SPBs for organization of an antiparallel microtubule array. Thus, we conclude that psr1p is involved in organizing the antiparallel microtubule array in the first place at mitosis onset by interaction with SUN/sad1p and EB1/mal3p, thereby establishing the bipolar spindle.postprin

Removal of antagonistic spindle forces can rescue metaphase spindle length and reduce chromosome segregation defects

Regular Abstracts - Tuesday Poster Presentations: no. 1925Metaphase describes a phase of mitosis where chromosomes are attached and oriented on the bipolar spindle for subsequent segregation at anaphase. In diverse cell types, the metaphase spindle is maintained at a relatively constant length. Metaphase spindle length is proposed to be regulated by a balance of pushing and pulling forces generated by distinct sets of spindle microtubules and their interactions with motors and microtubule-associated proteins (MAPs). Spindle length appears important for chromosome segregation fidelity, as cells with shorter or longer than normal metaphase spindles, generated through deletion or inhibition of individual mitotic motors or MAPs, showed chromosome segregation defects. To test the force balance model of spindle length control and its effect on chromosome segregation, we applied fast microfluidic temperature-control with live-cell imaging to monitor the effect of switching off different combinations of antagonistic forces in the fission yeast metaphase spindle. We show that spindle midzone proteins kinesin-5 cut7p and microtubule bundler ase1p contribute to outward pushing forces, and spindle kinetochore proteins kinesin-8 klp5/6p and dam1p contribute to inward pulling forces. Removing these proteins individually led to aberrant metaphase spindle length and chromosome segregation defects. Removing these proteins in antagonistic combination rescued the defective spindle length and, in some combinations, also partially rescued chromosome segregation defects. Our results stress the importance of proper chromosome-to-microtubule attachment over spindle length regulation for proper chromosome segregation.postprin