22 research outputs found

    Hands-on Science. Celebrating Science and Science Education

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    The book herein aims to contribute to the improvement of Science Education in our schools and to an effective implementation of a sound widespread scientific literacy at all levels of society

    Hands-on science. Rethinking STEAM education in times of uncertainty

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    After over two years of major constraints imposed by the COVID pandemic, the education world is still trying to find ways to adapt in order to keep providing, in an effective way, its crucial contribution to the world’ development our societies need and expect

    Ultrasensitive detection of toxocara canis excretory-secretory antigens by a nanobody electrochemical magnetosensor assay.

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    peer reviewedHuman Toxocariasis (HT) is a zoonotic disease caused by the migration of the larval stage of the roundworm Toxocara canis in the human host. Despite of being the most cosmopolitan helminthiasis worldwide, its diagnosis is elusive. Currently, the detection of specific immunoglobulins IgG against the Toxocara Excretory-Secretory Antigens (TES), combined with clinical and epidemiological criteria is the only strategy to diagnose HT. Cross-reactivity with other parasites and the inability to distinguish between past and active infections are the main limitations of this approach. Here, we present a sensitive and specific novel strategy to detect and quantify TES, aiming to identify active cases of HT. High specificity is achieved by making use of nanobodies (Nbs), recombinant single variable domain antibodies obtained from camelids, that due to their small molecular size (15kDa) can recognize hidden epitopes not accessible to conventional antibodies. High sensitivity is attained by the design of an electrochemical magnetosensor with an amperometric readout with all components of the assay mixed in one single step. Through this strategy, 10-fold higher sensitivity than a conventional sandwich ELISA was achieved. The assay reached a limit of detection of 2 and15 pg/ml in PBST20 0.05% or serum, spiked with TES, respectively. These limits of detection are sufficient to detect clinically relevant toxocaral infections. Furthermore, our nanobodies showed no cross-reactivity with antigens from Ascaris lumbricoides or Ascaris suum. This is to our knowledge, the most sensitive method to detect and quantify TES so far, and has great potential to significantly improve diagnosis of HT. Moreover, the characteristics of our electrochemical assay are promising for the development of point of care diagnostic systems using nanobodies as a versatile and innovative alternative to antibodies. The next step will be the validation of the assay in clinical and epidemiological contexts

    SPATIAL TRANSFORMATION PATTERN DUE TO COMMERCIAL ACTIVITY IN KAMPONG HOUSE

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    ABSTRACT Kampung houses are houses in kampung area of the city. Kampung House oftenly transformed into others use as urban dynamics. One of the transfomation is related to the commercial activities addition by the house owner. It make house with full private space become into mixused house with more public spaces or completely changed into full public commercial building. This study investigate the spatial transformation pattern of the kampung houses due to their commercial activities addition. Site observations, interviews and questionnaires were performed to study the spatial transformation. This study found that in kampung houses, the spatial transformation pattern was depend on type of commercial activities and owner perceptions, and there are several steps of the spatial transformation related the commercial activity addition. Keywords: spatial transformation pattern; commercial activity; owner perception, kampung house; adaptabilit

    Hands-on science: science education with and for society

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    The decisive importance of Science on the development of modern societies gives Science Education a role of special impact. Society sets the requirements rules and procedures of Education defining what concepts and competencies citizens must learn and how this learning should take place. Educational policies set by governments, elected and or imposed, not always reflects the will and ruling of Society. The School as pivotal element of our modern educational system must look behind and beyond imposed rules and regulations and persistently seek a permanent and open relation with Society, in all its dimensions, assuming and defending its crucial role on the development of Society and humankind. Aiming to contribute to an effective implementation of a sound widespread scientific literacy and effective Science Education in our Schools and Society at large, the Hands-on Science Network promotes a number of meetings and conferences open to the widest range of contributions on different pedagogic approaches with the common goal of promoting an effective learning of Science. This book gathers a number of interesting works presented at the 11th International Conference on Hands-on Science held in Aveiro, Portugal, July 21 to 25, 2014. The different chapters covers a wide range of topics including different strategies on connecting school’ science education with society and on synergetic relations between Society and Science Education, reports on good practices on formal as well as non-formal or informal science education, ICT tools, IBSE, active learning and hands-on pedagogy. We believe that the materials herein are a rather useful tool to assist teachers and educators as well as all interested in Science Education and its impact on the development of our Societies
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