Structural characterization and statistical-mechanical model of epidermal patterns

In proliferating epithelia of mammalian skin, cells of irregular polygonal-like shapes pack into complex nearly flat two-dimensional structures that are pliable to deformations. In this work, we employ various sensitive correlation functions to quantitatively characterize structural features of evolving packings of epithelial cells across length scales in mouse skin. We find that the pair statistics in direct and Fourier spaces of the cell centroids in the early stages of embryonic development show structural directional dependence, while in the late stages the patterns tend towards statistically isotropic states. We construct a minimalist four-component statistical-mechanical model involving effective isotropic pair interactions consisting of hard-core repulsion and extra short-ranged soft-core repulsion beyond the hard core, whose length scale is roughly the same as the hard core. The model parameters are optimized to match the sample pair statistics in both direct and Fourier spaces. By doing this, the parameters are biologically constrained. Our model predicts essentially the same polygonal shape distribution and size disparity of cells found in experiments as measured by Voronoi statistics. Moreover, our simulated equilibrium liquid-like configurations are able to match other nontrivial unconstrained statistics, which is a testament to the power and novelty of the model. We discuss ways in which our model might be extended so as to better understand morphogenesis (in particular the emergence of planar cell polarity), wound-healing, and disease progression processes in skin, and how it could be applied to the design of synthetic tissues

Time in quantum gravity

Quantum gravity--the marriage of quantum physics with general relativity--is bound to contain deep and important lessons for the nature of physical time. Some of these lessons shall be canvassed here, particularly as they arise from quantum general relativity and string theory and related approaches. Of particular interest is the question of which of the intuitive aspects of time will turn out to be fundamental, and which 'emergent' in some sense.Comment: 18 pages, 1 figur

Self-Calibration of Multi-Camera Systems for Vehicle Surround Sensing

Multikamerasysteme werden heute bereits in einer Vielzahl von Fahrzeugen und mobilen Robotern eingesetzt. Die Anwendungen reichen dabei von einfachen Assistenzfunktionen wie der Erzeugung einer virtuellen Rundumsicht bis hin zur Umfelderfassung, wie sie für teil- und vollautomatisches Fahren benötigt wird. Damit aus den Kamerabildern metrische Größen wie Distanzen und Winkel abgeleitet werden können und ein konsistentes Umfeldmodell aufgebaut werden kann, muss das Abbildungsverhalten der einzelnen Kameras sowie deren relative Lage zueinander bekannt sein. Insbesondere die Bestimmung der relativen Lage der Kameras zueinander, die durch die extrinsische Kalibrierung beschrieben wird, ist aufwendig, da sie nur im Gesamtverbund erfolgen kann. Darüber hinaus ist zu erwarten, dass es über die Lebensdauer des Fahrzeugs hinweg zu nicht vernachlässigbaren Veränderungen durch äußere Einflüsse kommt. Um den hohen Zeit- und Kostenaufwand einer regelmäßigen Wartung zu vermeiden, ist ein Selbstkalibrierungsverfahren erforderlich, das die extrinsischen Kalibrierparameter fortlaufend nachschätzt. Für die Selbstkalibrierung wird typischerweise das Vorhandensein überlappender Sichtbereiche ausgenutzt, um die extrinsische Kalibrierung auf der Basis von Bildkorrespondenzen zu schätzen. Falls die Sichtbereiche mehrerer Kameras jedoch nicht überlappen, lassen sich die Kalibrierparameter auch aus den relativen Bewegungen ableiten, die die einzelnen Kameras beobachten. Die Bewegung typischer Straßenfahrzeuge lässt dabei jedoch nicht die Bestimmung aller Kalibrierparameter zu. Um die vollständige Schätzung der Parameter zu ermöglichen, lassen sich weitere Bedingungsgleichungen, die sich z.B. aus der Beobachtung der Bodenebene ergeben, einbinden. In dieser Arbeit wird dazu in einer theoretischen Analyse gezeigt, welche Parameter sich aus der Kombination verschiedener Bedingungsgleichungen eindeutig bestimmen lassen. Um das Umfeld eines Fahrzeugs vollständig erfassen zu können, werden typischerweise Objektive, wie zum Beispiel Fischaugenobjektive, eingesetzt, die einen sehr großen Bildwinkel ermöglichen. In dieser Arbeit wird ein Verfahren zur Bestimmung von Bildkorrespondenzen vorgeschlagen, das die geometrischen Verzerrungen, die sich durch die Verwendung von Fischaugenobjektiven und sich stark ändernden Ansichten ergeben, berücksichtigt. Darauf aufbauend stellen wir ein robustes Verfahren zur Nachführung der Parameter der Bodenebene vor. Basierend auf der theoretischen Analyse der Beobachtbarkeit und den vorgestellten Verfahren stellen wir ein robustes, rekursives Kalibrierverfahren vor, das auf einem erweiterten Kalman-Filter aufbaut. Das vorgestellte Kalibrierverfahren zeichnet sich insbesondere durch die geringe Anzahl von internen Parametern, sowie durch die hohe Flexibilität hinsichtlich der einbezogenen Bedingungsgleichungen aus und basiert einzig auf den Bilddaten des Multikamerasystems. In einer umfangreichen experimentellen Auswertung mit realen Daten vergleichen wir die Ergebnisse der auf unterschiedlichen Bedingungsgleichungen und Bewegungsmodellen basierenden Verfahren mit den aus einer Referenzkalibrierung bestimmten Parametern. Die besten Ergebnisse wurden dabei durch die Kombination aller vorgestellten Bedingungsgleichungen erzielt. Anhand mehrerer Beispiele zeigen wir, dass die erreichte Genauigkeit ausreichend für eine Vielzahl von Anwendungen ist

Motion analysis report

Human motion analysis is the task of converting actual human movements into computer readable data. Such movement information may be obtained though active or passive sensing methods. Active methods include physical measuring devices such as goniometers on joints of the body, force plates, and manually operated sensors such as a Cybex dynamometer. Passive sensing de-couples the position measuring device from actual human contact. Passive sensors include Selspot scanning systems (since there is no mechanical connection between the subject's attached LEDs and the infrared sensing cameras), sonic (spark-based) three-dimensional digitizers, Polhemus six-dimensional tracking systems, and image processing systems based on multiple views and photogrammetric calculations

Self-Calibration of Multi-Camera Systems for Vehicle Surround Sensing

Multi-camera systems are being deployed in a variety of vehicles and mobile robots today. To eliminate the need for cost and labor intensive maintenance and calibration, continuous self-calibration is highly desirable. In this book we present such an approach for self-calibration of multi-Camera systems for vehicle surround sensing. In an extensive evaluation we assess our algorithm quantitatively using real-world data

Markerless deformation capture of hoverfly wings using multiple calibrated cameras

This thesis introduces an algorithm for the automated deformation capture of hoverfly wings from multiple camera image sequences. The algorithm is capable of extracting dense surface measurements, without the aid of fiducial markers, over an arbitrary number of wingbeats of hovering flight and requires limited manual initialisation. A novel motion prediction method, called the ‘normalised stroke model’, makes use of the similarity of adjacent wing strokes to predict wing keypoint locations, which are then iteratively refined in a stereo image registration procedure. Outlier removal, wing fitting and further refinement using independently reconstructed boundary points complete the algorithm. It was tested on two hovering data sets, as well as a challenging flight manoeuvre. By comparing the 3-d positions of keypoints extracted from these surfaces with those resulting from manual identification, the accuracy of the algorithm is shown to approach that of a fully manual approach. In particular, half of the algorithm-extracted keypoints were within 0.17mm of manually identified keypoints, approximately equal to the error of the manual identification process. This algorithm is unique among purely image based flapping flight studies in the level of automation it achieves, and its generality would make it applicable to wing tracking of other insects

Going beyond semantic image segmentation, towards holistic scene understanding, with associative hierarchical random fields

In this thesis we exploit the generality and expressive power of the Associative Hierarchical Random Field (AHRF) graphical model to take its use beyond that of semantic image segmentation, into object-classes, towards a framework for holistic scene understanding. We provide a working definition for the holistic approach to scene understanding, which allows for the integration of existing, disparate, applications into an unifying ensemble. We believe that modelling such an ensemble as an AHRF is both a principled and pragmatic solution. We present a hierarchy that shows several methods for fusing applications together with the AHRF graphical model. Each of the three; feature, potential and energy, layers subsumes its predecessor in generality and together give rise to many options for integration. With applications on street scenes we demonstrate an implementation of each layer. The first layer application joins appearance and geometric features. For our second layer we implement a things and stuff co-junction using higher order AHRF potentials for object detectors, with the goal of answering the classic questions: What? Where? and How many? A holistic approach to recognition-and-reconstruction is realised within our third layer by linking two energy based formulations of both applications. Each application is evaluated qualitatively and quantitatively. In all cases our holistic approach shows improvement over baseline methods

Bundle Block Adjustment with Constrained Relative Orientations

This paper deals with bundle adjustment with constrained cameras, i.e. where the orientation of certain cameras is expressed relatively to others, and these relative orientations are part of the unknowns. Despite the remarkable interest for oblique multi-camera systems, an empirical study on the effect of enforcing relative orientation constraints in bundle adjustment is still missing. We provide experimental evidence that indeed these constraints improve the accuracy of the results, while reducing the computational load as well. Moreover, we report for the first time in the literature the complete derivation of the Jacobian matrix for bundle adjustment with constrained cameras, to foster other implementations