20,353 research outputs found

    Molecular role of alphaB-crystallin in hypoxic retinal pigment epithelium

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    Molecular role of Crystallins in hypoxic Retinal Pigment Epithelium Background: Age-Related Macular Degeneration (AMD) causes gradual breakdown of vision due to insoluble deposits accumulation, called ”drusen”. The macula, a area near the center of the retina, holds the highest concentration of light-sensing cells that provide the image which we see. There are two kinds of AMD conditions, ”dry” and ”wet”. We focus on wet form, that is characterized by the presence of drusen between the retinal pigment epithelium (RPE) and Bruch's membrane, concomitantly with choroidal neovascularization (CNV) and is designated neovascular AMD (nAMD). During nAMD the retinal pigment epithelium (RPE) undergoes a hypoxic state (low oxygen), which causes growth of blood vessels to improve oxygen supply. Cells deficient in oxygen produce hypoxia-inducible factor (HIF-1), a transcription factor responsible for the upregulation of a multitude of angiogenic factors in response to hypoxia. Chaperones are a group of proteins assisting protein folding in cells under physiological and stress conditions. Chaperones recognize and bind proteins in their native form thus preventing unspecific aggregation. Small Heat-Shock Proteins (HSP) 20kd (HSP20) is a protein belonging to the family of chaperones; these are also known as alpha Crystallins. ARPE-19 is a human retinal pigment epithelial cell line which will be exposed to shock treatments, causing crystallin reactions. Cas9/CRISPR technologies can be used to generate crystallin mutant cells to deepen our hypothesis and methodology. Aims: The purpose of the present study will be to address the role alpha-B Crystallin in ARPE-19 cells exposed to hypoxia. Hypothesis: The hypothesis is that there is an interaction with crystallins and the Hypoxia-inducible factors (HIF) pathway. Standing question: how do crystallins interact with HIF pathway protein members, as well as their functional role and implication

    Gene Editing in pig models of inherited retinal diseases

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    Age exacerbates the effect of myopia on retinal capillaries and string vessels

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    The retinal vasculature supplies oxygen and nutrition to the cells and is crucial for an adequate retinal function. In myopia, excessive eye growth is associated with various anatomical changes that can lead to myopia-related complications. However, how myopia-induced ocular growth affects the integrity of the aged retinal microvasculature at the cellular level is not well understood. Here, we studied how aging interacts with myopia-induced alteration of the retinal microvasculature in fourteen marmoset retinas (Callithrix jacchus). String vessel and capillary branchpoint were imaged and quantified in all four capillary plexi of the retinal vasculature. As marmosets with lens-induced myopia aged, they developed increasing numbers of string vessels in all four vascular plexi, with increased vessel branchpoints in the parafoveal and peripapillary retina and decreased vessel branchpoints in the peripheral retina. These myopia-induced changes to the retinal microvasculature suggest an adaptive reorganization of the retinal microvascular cellular structure template with aging and during myopia development and progression

    a systematic review and meta-analysis

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    Background: Retinal toxicity with long-term hydroxychloroquine (HCQ) treatment is a major concern. This systematic review aims to assess the application of optical coherence tomography angiography (OCTA) to detect microvascular alterations in patients under HCQ. Methods: PubMed, Scopus, Web of Science, and Cochrane Library databases were systematically searched until January 14, 2023. Studies using OCTA as a primary diagnostic method to evaluate the macular microvasculature of HCQ users were included. Primary outcomes were macular vessel density (VD) and foveal avascular zone (FAZ) at the superficial (SCP) and deep (DCP) capillary plexus. Meta-analysis was performed using a random-effects model. Results: Of 211 screened abstracts, 13 were found eligible, enrolling 989 eyes from 778 patients. High-risk patients due to longer duration of treatment presented lower VD in the retinal microvasculature than those with low-risk in SCP (P = 0.02 in fovea; P = 0.004 in parafovea) and in DCP (P = 0.007 in fovea; P = 0.01 in parafovea). When compared with healthy controls, HCQ users had lower VD in both plexus—no quantitative synthesis was presented. Conclusions: Microvascular changes were found in autoimmune patients under HCQ treatment without any documented retinopathy. However, the evidence produced so far does not allow to draw conclusion concerning the effect of drug as studies were not controlled for disease duration. [Figure not available: see fulltext.].publishersversionepub_ahead_of_prin

    An Efficient DenseNet for Diabetic Retinopathy Screening

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    This study aims to propose a novel deep learning framework, i.e., efficient DenseNet, for identifying diabetic retinopathy severity levels in retinal images. Diabetic retinopathy is an eye condition that damages blood vessels in the retina. Detecting diabetic retinopathy at the early stage can avoid retinal detachment and effects leading to blindness in diabetic adults. A thin-layered efficient DenseNet model has been proposed with fewer training learnable parameters, leading to higher classification accuracy than the other deep learning models. The proposed deep learning framework for diabetic retinopathy severity level detection has an inbuilt automatic pre-processing module. Afterward, the efficient DenseNet model and classifier will provide data augmentation and higher-level feature extraction. The proposed efficient DenseNet framework is trained and tested using 13000 retinal fundus images within the diabetic retinopathy database and combined with the k-nearest neighbor classifier demonstrating the best classification accuracy of 98.40%

    Influencing factors associated with high myopia in Chinese college students

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    BackgroundHigh myopia (HM) may elicit irreversible pathological changes in the fundus and severely impair visual quality, thereby becoming a major public health issue in China. However, the influencing factors associated with HM remain unknown in Chinese college students, whose visual quality is crucial to country development.MethodsThis is a cross-sectional observational study. Two thousand three hundred and fifteen undergraduate and graduate students were initially recruited from various majors in 3 universities in Tianjin, China. Under the principle of voluntary participation and informed consent, simple random sampling was conducted in the recruited subjects while maintaining balanced number of subjects from each major. After screening with inclusion and exclusion criteria, 96 undergraduate and graduate students (186 eyes) were finally included and divided into non-HM and HM groups. The eyes of subjects were examined by optical coherence tomography angiography (OCTA) for vessel density and structure thickness at the macula and optic disc, and the subjects were surveyed by an itemized questionnaire on lifestyles and study habits.ResultsThe OCTA and questionnaire results revealed 10 factors, including hemodynamic and anatomic parameters and lifestyle metrics, with statistical significance between the non-HM and HM groups. Receiver operating characteristic curve analysis showed that vessel density of the inner retina at the macula, vessel density of the radial peripapillary capillary at the optic disc, smartphone usage time, continuous near work time, and sleeping after midnight had superior values of area under the curve (AUC > 0.700). Therefore, these 5 factors were selected for univariant and multivariant logistic regression analyses. A prediction model comprising the 5 influencing factors had an AUC of 0.940 and 95% CI of 0.908–0.972.ConclusionThis study for the first time identified the vessel density of the inner retina at the macula, the vessel density of the radial peripapillary capillary at the optic disc, smartphone usage time, continuous near work time, and sleeping after midnight as influencing factors associated with HM in Chinese college students. A prediction model comprising the 5 influencing factors was proposed for calculating likelihood of a Chinese college student developing HM, based on which lifestyle improvement and medical intervention might be recommended

    Study and Analysis of Fluid Filled Abnormalities in Retina Using OCT Images

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    Visual impairment is one of the most regularly happening infections in human. The reason being variation from the normal in the different layers of retina because of strange measure of liquid either abundance aggregation or shortage. This paper targets recognizing and assessing the different abnormalities that could be earlier stages to visual deficiency. The proposed target is achieved by means of implementation using Digital Image Processing Technique, starting from preprocessing to classification at various stages. Not restricting to binary classification as normal or abnormal, the proposed system also extends its capacity to classify the input image as Cystoid Macular Edema (CME), Choroidal Neo Vascular Membrane (CNVM), Macular Hole (MH) and normal images. The preprocessing methodology implemented filters to remove the speckle noises which are most common in ultrasound-based imaging system. Random forest classifier was utilized for classifying the input features and also seems to be promising on par with the various existing methodologies

    The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans

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    Hair cell (HC) loss by epithelial extrusion has been described to occur in the rodent vestibular system during chronic 3,3'-iminodipropionitrile (IDPN) ototoxicity. This is preceded by dismantlement of the calyceal junction in the contact between type I HC (HCI) and calyx afferent terminals. Here, we evaluated whether these phenomena have wider significance. First, we studied rats receiving seven different doses of streptomycin, ranging from 100 to 800 mg/kg/day, for 3 to 8 weeks. Streptomycin caused loss of vestibular function associated with partial loss of HCI and decreased expression of contactin-associated protein (CASPR1), denoting calyceal junction dismantlement, in the calyces encasing the surviving HCI. Additional molecular and ultrastructural data supported the conclusion that HC-calyx detachment precede HCI loss by extrusion. Animals allowed to survive after the treatment showed functional recuperation and rebuilding of the calyceal junction. Second, we evaluated human sensory epithelia obtained during therapeutic labyrinthectomies and trans-labyrinthine tumour excisions. Some samples showed abnormal CASPR1 label strongly suggestive of calyceal junction dismantlement. Therefore, reversible dismantlement of the vestibular calyceal junction may be a common response triggered by chronic stress, including ototoxic stress, before HCI loss. This may partly explain clinical observations of reversion in function loss after aminoglycoside exposure
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