Interactive Visual Analysis of Translations

This thesis is the result of a collaboration with the College of Arts and Humanities at Swansea University. The goal of this collaboration is to design novel visualization techniques to enable digital humanities scholars to explore and analyze parallel translations. To this end, chapter 2 introduces the first survey of surveys on text visualization which reviews all of the surveys and state-of-the-art reports on text visualization techniques, classifies them, provides recommendations, and discusses reported challenges.Following this, we present three visual interactive designs that support the typical digital humanities scholars workflow. In Chapter 4, we present VNLP, a visual, interactive design that enables users to explicitly observe the NLP pipeline processes and update the parameters at each processing stage. Chapter 5 presents AlignVis, a visual tool that provides a semi-automatic alignment framework to build a correspondence between multiple translations. It presents the results of using text similarity measurements and enables the user to create, verify, and edit alignments using a novel visual interface. Chapter 6 introduce TransVis, a novel visual design that supports comparison of multiple parallel translations. It incorporates customized mechanisms for rapid and interactive filtering and selection of a large number of German translations of Shakespeare’s Othello. All of the visual designs are evaluated using examples, detailed observations, case studies, and/or domain expert feedback from a specialist in modern and contemporary German literature and culture.Chapter 7 reports our collaborative experience and proposes a methodological workflow to guide such interdisciplinary research projects. This chapter also includes a summary of outcomes and lessons learned from our collaboration with the domain expert. Finally, Chapter 8 presents a summary of the thesis and future work directions

Earth Observation Open Science and Innovation

geospatial analytics; social observatory; big earth data; open data; citizen science; open innovation; earth system science; crowdsourced geospatial data; citizen science; science in society; data scienc

New Methods to Improve Large-Scale Microscopy Image Analysis with Prior Knowledge and Uncertainty

Multidimensional imaging techniques provide powerful ways to examine various kinds of scientific questions. The routinely produced data sets in the terabyte-range, however, can hardly be analyzed manually and require an extensive use of automated image analysis. The present work introduces a new concept for the estimation and propagation of uncertainty involved in image analysis operators and new segmentation algorithms that are suitable for terabyte-scale analyses of 3D+t microscopy images

New Methods to Improve Large-Scale Microscopy Image Analysis with Prior Knowledge and Uncertainty

Multidimensional imaging techniques provide powerful ways to examine various kinds of scientific questions. The routinely produced datasets in the terabyte-range, however, can hardly be analyzed manually and require an extensive use of automated image analysis. The present thesis introduces a new concept for the estimation and propagation of uncertainty involved in image analysis operators and new segmentation algorithms that are suitable for terabyte-scale analyses of 3D+t microscopy images.Comment: 218 pages, 58 figures, PhD thesis, Department of Mechanical Engineering, Karlsruhe Institute of Technology, published online with KITopen (License: CC BY-SA 3.0, http://dx.doi.org/10.5445/IR/1000057821

2023 IMSAloquium

Welcome to IMSAloquium 2023. This is IMSA’s 36 th year of leading in educationalinnovation, and the 35th year of the IMSA Student Inquiry and Research (SIR) Program.https://digitalcommons.imsa.edu/archives_sir/1033/thumbnail.jp

Whole-body integration of gene expression and single-cell morphology

Animal bodies are composed of cell types with unique expression programs that implement their distinct locations, shapes, structures, and functions. Based on these properties, cell types assemble into specific tissues and organs. To systematically explore the link between cell-type-specific gene expression and morphology, we registered an expression atlas to a whole-body electron microscopy volume of the nereid Platynereis dumerilii. Automated segmentation of cells and nuclei identifies major cell classes and establishes a link between gene activation, chromatin topography, and nuclear size. Clustering of segmented cells according to gene expression reveals spatially coherent tissues. In the brain, genetically defined groups of neurons match ganglionic nuclei with coherent projections. Besides interneurons, we uncover sensory-neurosecretory cells in the nereid mushroom bodies, which thus qualify as sensory organs. They furthermore resemble the vertebrate telencephalon by molecular anatomy. We provide an integrated browser as a Fiji plugin for remote exploration of all available multimodal datasets

Coordination Mechanisms of Mammalian Embryo Implantation

A direct interaction between the extraembryonic and the uterine tissues during embryo implantation generates a unique biomechanical context for the blastocyst. However, our mechanistic understanding of the regulation of blastocyst morphogenesis during implantation is limited by the inaccessibility in vivo and remaining challenges to model feto-maternal interaction ex vivo. To overcome these limitations, I applied microfabrication and biomaterial engineering to model biomechanical cues of the murine intrauterine environment ex vivo with high precision and tunability. I identify that embryo-uterine adhesion and tissue geometry are critical for successful peri-implantation development. In a specific parameter range, closely resembling in utero conditions, the 3D geometrically patterned hydrogel supports mouse blastocysts through implantation and enables robust peri-implantation morphogenesis; promotes the development of the Reichert’s membrane and all extraembryonic tissues, including giant trophoblast, which directly interacts with the uterus. To monitor in toto peri-implantation embryo dynamics, the culture method was integrated with inverted view InVi-SPIM and multiview MuVi-SPIM light-sheet microscopes. I show that integrin-mediated adhesion by the mural trophectoderm provides the mechanism of trophectoderm tension release, driving the morphogenesis of the extraembryonic ectoderm and egg cylinder patterning. Moreover, the embryo-uterine adhesion enables collective trophoblast migration, dependent on Rac1. Finally, I demonstrate that the uterine tissue geometry spatially coordinates collective trophoblast migration to delineate space for egg cylinder growth. Together, this study reveals essential mechanisms of dynamic embryo-uterus interactions during peri-implantation development

From nanometers to centimeters: Imaging across spatial scales with smart computer-aided microscopy

Microscopes have been an invaluable tool throughout the history of the life sciences, as they allow researchers to observe the miniscule details of living systems in space and time. However, modern biology studies complex and non-obvious phenotypes and their distributions in populations and thus requires that microscopes evolve from visual aids for anecdotal observation into instruments for objective and quantitative measurements. To this end, many cutting-edge developments in microscopy are fuelled by innovations in the computational processing of the generated images. Computational tools can be applied in the early stages of an experiment, where they allow for reconstruction of images with higher resolution and contrast or more colors compared to raw data. In the final analysis stage, state-of-the-art image analysis pipelines seek to extract interpretable and humanly tractable information from the high-dimensional space of images. In the work presented in this thesis, I performed super-resolution microscopy and wrote image analysis pipelines to derive quantitative information about multiple biological processes. I contributed to studies on the regulation of DNMT1 by implementing machine learning-based segmentation of replication sites in images and performed quantitative statistical analysis of the recruitment of multiple DNMT1 mutants. To study the spatiotemporal distribution of DNA damage response I performed STED microscopy and could provide a lower bound on the size of the elementary spatial units of DNA repair. In this project, I also wrote image analysis pipelines and performed statistical analysis to show a decoupling of DNA density and heterochromatin marks during repair. More on the experimental side, I helped in the establishment of a protocol for many-fold color multiplexing by iterative labelling of diverse structures via DNA hybridization. Turning from small scale details to the distribution of phenotypes in a population, I wrote a reusable pipeline for fitting models of cell cycle stage distribution and inhibition curves to high-throughput measurements to quickly quantify the effects of innovative antiproliferative antibody-drug-conjugates. The main focus of the thesis is BigStitcher, a tool for the management and alignment of terabyte-sized image datasets. Such enormous datasets are nowadays generated routinely with light-sheet microscopy and sample preparation techniques such as clearing or expansion. Their sheer size, high dimensionality and unique optical properties poses a serious bottleneck for researchers and requires specialized processing tools, as the images often do not fit into the main memory of most computers. BigStitcher primarily allows for fast registration of such many-dimensional datasets on conventional hardware using optimized multi-resolution alignment algorithms. The software can also correct a variety of aberrations such as fixed-pattern noise, chromatic shifts and even complex sample-induced distortions. A defining feature of BigStitcher, as well as the various image analysis scripts developed in this work is their interactivity. A central goal was to leverage the user's expertise at key moments and bring innovations from the big data world to the lab with its smaller and much more diverse datasets without replacing scientists with automated black-box pipelines. To this end, BigStitcher was implemented as a user-friendly plug-in for the open source image processing platform Fiji and provides the users with a nearly instantaneous preview of the aligned images and opportunities for manual control of all processing steps. With its powerful features and ease-of-use, BigStitcher paves the way to the routine application of light-sheet microscopy and other methods producing equally large datasets