397,623 research outputs found
Multi-view passive 3D face acquisition device
Approaches to acquisition of 3D facial data include laser scanners, structured
light devices and (passive) stereo vision. The laser scanner and structured light
methods allow accurate reconstruction of the 3D surface but strong light is projected
on the faces of subjects. Passive stereo vision based approaches do not require strong
light to be projected, however, it is hard to obtain comparable accuracy and robustness
of the surface reconstruction. In this paper a passive multiple view approach using
5 cameras in a ’+’ configuration is proposed that significantly increases robustness
and accuracy relative to traditional stereo vision approaches. The normalised cross
correlations of all 5 views are combined using direct projection of points instead of
the traditionally used rectified images. Also, errors caused by different perspective
deformation of the surface in the different views are reduced by using an iterative reconstruction
technique where the depth estimation of the previous iteration is used to
warp the windows of the normalised cross correlation for the different views
Robustness from flexibility in the fungal circadian clock
Background
Robustness is a central property of living systems, enabling function to be maintained against environmental perturbations. A key challenge is to identify the structures in biological circuits that confer system-level properties such as robustness. Circadian clocks allow organisms to adapt to the predictable changes of the 24-hour day/night cycle by generating endogenous rhythms that can be entrained to the external cycle. In all organisms, the clock circuits typically comprise multiple interlocked feedback loops controlling the rhythmic expression of key genes. Previously, we showed that such architectures increase the flexibility of the clock's rhythmic behaviour. We now test the relationship between flexibility and robustness, using a mathematical model of the circuit controlling conidiation in the fungus Neurospora crassa.
Results
The circuit modelled in this work consists of a central negative feedback loop, in which the frequency (frq) gene inhibits its transcriptional activator white collar-1 (wc-1), interlocked with a positive feedback loop in which FRQ protein upregulates WC-1 production. Importantly, our model reproduces the observed entrainment of this circuit under light/dark cycles with varying photoperiod and cycle duration. Our simulations show that whilst the level of frq mRNA is driven directly by the light input, the falling phase of FRQ protein, a molecular correlate of conidiation, maintains a constant phase that is uncoupled from the times of dawn and dusk. The model predicts the behaviour of mutants that uncouple WC-1 production from FRQ's positive feedback, and shows that the positive loop enhances the buffering of conidiation phase against seasonal photoperiod changes. This property is quantified using Kitano's measure for the overall robustness of a regulated system output. Further analysis demonstrates that this functional robustness is a consequence of the greater evolutionary flexibility conferred on the circuit by the interlocking loop structure.
Conclusions
Our model shows that the behaviour of the fungal clock in light-dark cycles can be accounted for by a transcription-translation feedback model of the central FRQ-WC oscillator. More generally, we provide an example of a biological circuit in which greater flexibility yields improved robustness, while also introducing novel sensitivity analysis techniques applicable to a broader range of cellular oscillators
Nonlinear Michelson interferometer for improved quantum metrology
We examine quantum detection via a Michelson interferometer embedded in a gas
with Kerr nonlinearity. This nonlinear interferometer is illuminated by pulses
of classical light. This strategy combines the robustness against practical
imperfections of classical light with the improvement provided by nonlinear
processes. Regarding ultimate quantum limits, we stress that, as a difference
with linear schemes, the nonlinearity introduces pulse duration as a new
variable into play along with the energy resources.Comment: 5 pages, 1 figure. Close to published versio
Sub-Doppler resonances in the back-scattered light from random porous media infused with Rb vapor
We report on the observation of sub-Doppler resonances on the back-scattered
light from a random porous glass medium with rubidium vapor filling its
interstices. The sub-Doppler spectral lines are the consequence of saturated
absorption where the incident laser beam saturates the atomic medium and the
back-scattered light probes it. Some specificities of the observed spectra
reflect the transient atomic evolution under confinement inside the pores.
Simplicity, robustness and potential miniaturization are appealing features of
this system as a spectroscopic reference.Comment: 6 pages, 4 figure
A Realistic Model under which the Genetic Code is Optimal
The genetic code has a high level of error robustness. Using values of
hydrophobicity scales as a proxy for amino acid character, and the Mean Square
measure as a function quantifying error robustness, a value can be obtained for
a genetic code which reflects the error robustness of that code. By comparing
this value with a distribution of values belonging to codes generated by random
permutations of amino acid assignments, the level of error robustness of a
genetic code can be quantified. We present a calculation in which the standard
genetic code is shown to be optimal. We obtain this result by (1) using
recently updated values of polar requirement as input; (2) fixing seven
assignments (Ile, Trp, His, Phe, Tyr, Arg, and Leu) based on aptamer
considerations; and (3) using known biosynthetic relations of the 20 amino
acids. This last point is reflected in an approach of subdivision (restricting
the random reallocation of assignments to amino acid subgroups, the set of 20
being divided in four such subgroups). The three approaches to explain
robustness of the code (specific selection for robustness, amino acid-RNA
interactions leading to assignments, or a slow growth process of assignment
patterns) are reexamined in light of our findings. We offer a comprehensive
hypothesis, stressing the importance of biosynthetic relations, with the code
evolving from an early stage with just glycine and alanine, via intermediate
stages, towards 64 codons carrying todays meaning.Comment: 22 pages, 3 figures, 4 tables Journal of Molecular Evolution, July
201
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