Nano handling and measurement of biological cells in culture

A thesis submitted to the University of Bedfordshire in partial fulfillment of the requirements for the degree of Doctor of PhilosophyThis thesis systematically investigates the nano handling and measurement techniques for biological cells in culture and studies the techniques to realize innovative and multi-functional applications in biomedicine. Among them, the technique based on AFM is able to visualize and quantify the dynamics of organic cells in culture on the nano scale. Especially, the cellular shear adhesion force on the various locations of biological cells was firstly accurately measured in the research of the cell-substrate interaction in terms of biophysical perspective. The innovative findings are conductive to study the cell-cell adhesion, the cell-matrix adhesion which is related to the cell morphology structure, function, deformation ability and adhesion of cells and better understand the cellular dynamic behaviors. Herein, a new liquid-AFM probe unit and an increment PID control algorithm were implemented suitable for scanning the cell samples under the air conditions and the liquid environments. The influence between the surface of sample and the probe, and the damage of probe during the sample scanning were reduced. The proposed system is useful for the nano handling and measurement of living cells. Besides, Besides, to overcome the limitations of liquid-AFMs, the multiple optical tweezers were developed to integrate with the liquid-AFM. The technique based on laser interference is able to characterize the optical trap stiffness and the escape velocity, especially to realize the capture and sorting of multiple cells by a polarization-controlled periodic laser interference. It can trap and move hundreds of cells without physical contact, and has broad application prospects in cytology. Herein, a new experimental method integrated with the positioning analysis in the Z direction was used to improve the fluid force method for the calibration and characterize the mechanical forces exerted on optical traps and living cells. Moreover, a sensitive and highly efficient polarization-controlled three-beam interference set-up was developed for the capture and sorting of multiple cells. By controlling the polarization angles of the beams, various intensity distributions and different sizes of dots were obtained. Subsequently, we have experimentally observed multiple optical tweezers and the sorting of cells with different polarization angles, which are in accordance with the theoretical analysis

Nondestructive Testing (NDT)

The aim of this book is to collect the newest contributions by eminent authors in the field of NDT-SHM, both at the material and structure scale. It therefore provides novel insight at experimental and numerical levels on the application of NDT to a wide variety of materials (concrete, steel, masonry, composites, etc.) in the field of Civil Engineering and Architecture

Bio-Radar: sistema de aquisição de sinais vitais sem contacto

The Bio-Radar system is capable to measure vital signs accurately, namely the respiratory and cardiac signal, using electromagnetic waves. In this way, it is possible to monitor subjects remotely and comfortably for long periods of time. This system is based on the micro-Doppler effect, which relates the received signal phase variation with the distance change between the subject chest-wall and the radar antennas, which occurs due to the cardiopulmonary function. Considering the variety of applications where this system can be used, it is required to evaluate its performance when applied to real context scenarios and thus demonstrate the advantages that bioradar systems can bring to the general population. In this work, a bio-radar prototype was developed in order to verify the viability to be integrated in specific applications, using robust and low profile solutions that equally guarantee the general system performance while addressing the market needs. Considering these two perspectives to be improved, different level solutions were developed. On the hardware side, textile antennas were developed to be embedded in a car seat upholstery, thus reaching a low profile solution and easy to include in the industrialization process. Real context scenarios imply long-term monitoring periods, where involuntary body motion can occur producing high amplitude signals that overshadow the vital signs. Non-controlled monitoring environments might also produce time varying parasitic reflections that have a direct impact in the signal. Additionally, the subject's physical stature and posture during the monitoring period can have a different impact in the signals quality. Therefore, signal processing algorithms were developed to be robust to low quality signals and non-static scenarios. On the other hand, the bio-radar potential can also be maximized if the acquired signals are used pertinently to help identify the subject's psychophysiological state enabling one to act accordingly. The random body motion until now has been seen as a noisy source, however it can also provide useful information regarding subject's state. In this sense, the acquired vital signs as well as other body motions were used in machine learning algorithms with the goal to identify the subject's emotions and thus verify if the remotely acquired vital signs can also provide useful information.O sistema Bio-Radar permite medir sinais vitais com precisão, nomeadamente o sinal respiratório e cardíaco, utilizando ondas eletromagnéticas para esse fim. Desta forma, é possível monitorizar sujeitos de forma remota e confortável durante longos períodos de tempo. Este sistema é baseado no efeito de micro-Doppler, que relaciona a variação de fase do sinal recebido com a alteração da distância entre as antenas do radar e a caixa torácica do sujeito, que ocorre durante a função cardiopulmonar. Considerando a variedade de aplicações onde este sistema pode ser utilizado, é necessário avaliar o seu desempenho quando aplicado em contextos reais e assim demonstrar as vantagens que os sistemas bio-radar podem trazer à população geral. Neste trabalho, foi desenvolvido um protótipo do bio radar com o objetivo de verificar a viabilidade de integrar estes sistemas em aplicações específicas, utilizando soluções robustas e discretas que garantam igualmente o seu bom desempenho, indo simultaneamente de encontro às necessidades do mercado. Considerando estas duas perspetivas em que o sistema pode ser melhorado, foram desenvolvidas soluções de diferentes níveis. Do ponto de vista de hardware, foram desenvolvidas antenas têxteis para serem integradas no estofo de um banco automóvel, alcançando uma solução discreta e fácil de incluir num processo de industrialização. Contextos reais de aplicação implicam períodos de monitorização longos, onde podem ocorrer movimentos corporais involuntários que produzem sinais de elevada amplitude que se sobrepõem aos sinais vitais. Ambientes de monitorização não controlados podem produzir reflexões parasitas variantes no tempo que têm impacto direto no sinal. Adicionalmente, a estrutura física do sujeito e a sua postura durante o período de monitorização podem ter impactos diferentes na qualidade dos sinais. Desta forma, foram desenvolvidos algoritmos de processamento de sinal robustos a sinais de baixa qualidade e a cenários não estáticos. Por outro lado, o potencial do bio radar pode também ser maximizado se os sinais adquiridos forem pertinentemente utilizados de forma a ajudar a identificar o estado psicofisiológico do sujeito, permitindo mais tarde agir em conformidade. O movimento corporal aleatório que foi até agora visto como uma fonte de ruído, pode no entanto também fornecer informação útil sobre o estado do sujeito. Neste sentido, os sinais vitais e outros movimentos corporais adquiridos foram utilizados em algoritmos de aprendizagem automática com o objetivo de identificar as emoções do sujeito e assim verificar que sinais vitais adquiridos remotamente podem também conter informação útil.Programa Doutoral em Engenharia Eletrotécnic