Coastal wetland mapping with sentinel-2 MSI imagery based on gravitational optimized multilayer perceptron and morphological attribute profiles.

Coastal wetland mapping plays an essential role in monitoring climate change, the hydrological cycle, and water resources. In this study, a novel classification framework based on the gravitational optimized multilayer perceptron classifier and extended multi-attribute profiles (EMAPs) is presented for coastal wetland mapping using Sentinel-2 multispectral instrument (MSI) imagery. In the proposed method, the morphological attribute profiles (APs) are firstly extracted using four attribute filters based on the characteristics of wetlands in each band from Sentinel-2 imagery. These APs form a set of EMAPs which comprehensively represent the irregular wetland objects in multiscale and multilevel. The EMAPs and original spectral features are then classified with a new multilayer perceptron (MLP) classifier whose parameters are optimized by a stability-constrained adaptive alpha for a gravitational search algorithm. The performance of the proposed method was investigated using Sentinel-2 MSI images of two coastal wetlands, i.e., the Jiaozhou Bay and the Yellow River Delta in Shandong province of eastern China. Comparisons with four other classifiers through visual inspection and quantitative evaluation verified the superiority of the proposed method. Furthermore, the effectiveness of different APs in EMAPs were also validated. By combining the developed EMAPs features and novel MLP classifier, complicated wetland types with high within-class variability and low between-class disparity were effectively discriminated. The superior performance of the proposed framework makes it available and preferable for the mapping of complicated coastal wetlands using Sentinel-2 data and other similar optical imagery

clusterMaker: a multi-algorithm clustering plugin for Cytoscape

<p>Abstract</p> <p>Background</p> <p>In the post-genomic era, the rapid increase in high-throughput data calls for computational tools capable of integrating data of diverse types and facilitating recognition of biologically meaningful patterns within them. For example, protein-protein interaction data sets have been clustered to identify stable complexes, but scientists lack easily accessible tools to facilitate combined analyses of multiple data sets from different types of experiments. Here we present <it>clusterMaker</it>, a Cytoscape plugin that implements several clustering algorithms and provides network, dendrogram, and heat map views of the results. The Cytoscape network is linked to all of the other views, so that a selection in one is immediately reflected in the others. <it>clusterMaker </it>is the first Cytoscape plugin to implement such a wide variety of clustering algorithms and visualizations, including the only implementations of hierarchical clustering, dendrogram plus heat map visualization (tree view), k-means, k-medoid, SCPS, AutoSOME, and native (Java) MCL.</p> <p>Results</p> <p>Results are presented in the form of three scenarios of use: analysis of protein expression data using a recently published mouse interactome and a mouse microarray data set of nearly one hundred diverse cell/tissue types; the identification of protein complexes in the yeast <it>Saccharomyces cerevisiae</it>; and the cluster analysis of the vicinal oxygen chelate (VOC) enzyme superfamily. For scenario one, we explore functionally enriched mouse interactomes specific to particular cellular phenotypes and apply fuzzy clustering. For scenario two, we explore the prefoldin complex in detail using both physical and genetic interaction clusters. For scenario three, we explore the possible annotation of a protein as a methylmalonyl-CoA epimerase within the VOC superfamily. Cytoscape session files for all three scenarios are provided in the Additional Files section.</p> <p>Conclusions</p> <p>The Cytoscape plugin <it>clusterMaker </it>provides a number of clustering algorithms and visualizations that can be used independently or in combination for analysis and visualization of biological data sets, and for confirming or generating hypotheses about biological function. Several of these visualizations and algorithms are only available to Cytoscape users through the <it>clusterMaker </it>plugin. <it>clusterMaker </it>is available via the Cytoscape plugin manager.</p

Las nuevas construcciones antes la conservación del paisaje: un acercamiento a su estudio. Caso particular del páramo leonés

El paisaje es un recurso natural que cada vez aparece considerado con mayor profusión en la redacción de proyectos, y que debe ser protegido y conservado ante la construcción de Intervenciones antrópicas. Por tanto, el impacto visual ha de ser estudiado como una parte primordial en los estudios generales de impacto ambiental. Las nuevas tecnologías deben contribuir a ello proporcionando herramientas informáticas que realicen simulaciones y reproducciones de la realidad con alto grado de fidelidad

Las nuevas construcciones antes la conservación del paisaje: un acercamiento a su estudio. Caso particular del páramo leonés

El paisaje es un recurso natural que cada vez aparece considerado con mayor profusión en la redacción de proyectos, y que debe ser protegido y conservado ante la construcción de Intervenciones antrópicas. Por tanto, el impacto visual ha de ser estudiado como una parte primordial en los estudios generales de impacto ambiental. Las nuevas tecnologías deben contribuir a ello proporcionando herramientas informáticas que realicen simulaciones y reproducciones de la realidad con alto grado de fidelidad

Chemogenetic E-MAP in Saccharomyces cerevisiae for identification of membrane transporters operating lipid flip flop

While most yeast enzymes for the biosynthesis of glycerophospholipids, sphingolipids and ergosterol are known, genes for several postulated transporters allowing the flopping of biosynthetic intermediates and newly made lipids from the cytosolic to the lumenal side of the membrane are still not identified. An E-MAP measuring the growth of 142'108 double mutants generated by systematically crossing 543 hypomorphic or deletion alleles in genes encoding multispan membrane proteins, both on media with or without an inhibitor of fatty acid synthesis, was generated. Flc proteins, represented by 4 homologous genes encoding presumed FAD or calcium transporters of the ER, have a severe depression of sphingolipid biosynthesis and elevated detergent sensitivity of the ER. FLC1, FLC2 and FLC3 are redundant in granting a common function, which remains essential even when the severe cell wall defect of flc mutants is compensated by osmotic support. Biochemical characterization of some other genetic interactions shows that Cst26 is the enzyme mainly responsible for the introduction of saturated very long chain fatty acids into phosphatidylinositol and that the GPI lipid remodelase Cwh43, responsible for introducing ceramides into GPI anchors having a C26:0 fatty acid in sn-2 of the glycerol moiety can also use lyso-GPI protein anchors and various base resistant lipids as substrates. Furthermore, we observe that adjacent deletions in several chromosomal regions show strong negative genetic interactions with a single gene on another chromosome suggesting the presence of undeclared suppressor mutations in certain chromosomal regions that need to be identified in order to yield meaningful E-map data

Analysis of Visual Impact on the Landscape

Jedan od ključnih elemenata u zaštiti okoliša i sastavni dio kompleksnih procjena utjecaja na okolinu jesu i studije vizualnog utjecaja na okolinu. U radu su dane osnovne zakonske odrednice pri izradi studije, ponajprije znanstveni okvir i analiza rada pri izradi takve studije. Ovdje prikazana metodologija izrade rezultat je znanstvenog istraživanja i stečenih iskustava tijekom višegodišnjeg rada.Analyzing visual impact on the landscape is one of the key elements of environmental protection, and part of more complex environmental impact assessment. The article presents some legislative regulations for visual impact analysis, but mainly concentrates on the scientific framework and research necessary to develop it. The methodology presented is the result of research and experience in recent years

Analysis of Visual Impact on the Landscape

Jedan od ključnih elemenata u zaštiti okoliša i sastavni dio kompleksnih procjena utjecaja na okolinu jesu i studije vizualnog utjecaja na okolinu. U radu su dane osnovne zakonske odrednice pri izradi studije, ponajprije znanstveni okvir i analiza rada pri izradi takve studije. Ovdje prikazana metodologija izrade rezultat je znanstvenog istraživanja i stečenih iskustava tijekom višegodišnjeg rada.Analyzing visual impact on the landscape is one of the key elements of environmental protection, and part of more complex environmental impact assessment. The article presents some legislative regulations for visual impact analysis, but mainly concentrates on the scientific framework and research necessary to develop it. The methodology presented is the result of research and experience in recent years